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Overview,

Continued

The
GeneCatcher

™

Technology

The GeneCatcher

™

Technology is a novel magnetic bead-based technology that

is designed to work on a wide range of blood samples including archived or
poorly stored blood samples to facilitate genomic DNA purification.
See figure below for details.

Step 1

–DNA Capture–

Cells are lysed and
crude DNA is captured
on magnetic beads
leaving most of the cell
debris and protein
behind in solution.

Step 2

–DNA

Purification–Any
residual protein is
digested using the
protease and then
washed away to leave
pure intact DNA.

Step 3

–Elution–The pure

DNA is then eluted into
a small volume ready for
use in any downstream
applications.

Purpose of the
Manual

This manual provides the following information:

•

Details and specifications on the iPrep

™

Purification Instrument and

iPrep

™

Kits

•

Unpacking and installing the iPrep

™

Purification Instrument

•

Operating the iPrep

™

Instrument

•

Cleaning and maintaining the iPrep

™

Instrument

•

Troubleshooting

Specific purification protocols are included in the manuals supplied with
iPrep

™

Kits.

Содержание iPrep

Страница 1: ...T 1 760 603 7200 F 1 760 602 6500 E tech service invitrogen com For country speci c contact information visit our web site at www invitrogen com iPrep Purification Instrument For automated purificati...

Страница 2: ...ii...

Страница 3: ...nstrument xiii Accessory Products xvi Introduction 1 Overview 1 Description of Parts 5 Experimental Overview 12 Methods 13 General Guidelines 13 Getting Started 15 Using the iPrep Purification Instrum...

Страница 4: ...iv...

Страница 5: ...ation see the iPrep Kit manual 1 Press Start to run a protocol Select the desired purification protocol 2 Open the iPrep door Remove the iPrep Cartridge Rack and iPrep Tip and Tube Rack to set up the...

Страница 6: ...A as described in the iPrep Kit manual 14 Discard the used cartridges tips and tubes into biohazard waste Do not reuse the cartridges 15 To purify more samples using the same iPrep Card load the racks...

Страница 7: ...Upon Receiving the Instrument Examine the unit carefully for any damage incurred during transit Any damage claims must be filed with the carrier The warranty does not cover in transit damage iPrep St...

Страница 8: ...he symbols used on the iPrep Purification Instrument and in the manual are explained below High Temperature Caution Used near the heat block to indicate a warning resulting due to high temperature Bio...

Страница 9: ...see purification kit manual for details Dispensing Volume 5 1000 l Dispensing Accuracy Blue Tips 5 20 l Less than 15 CV 21 200 l Less than 2 CV DN100N Tips 25 50 l Less than 5 CV 50 1000 l Less than 2...

Страница 10: ...fication Instrument is subject to the conditions described in this manual The protection provided by the instrument may be impaired if the instrument is used in a manner not specified by Invitrogen Th...

Страница 11: ...Remove the wrapped instrument box from the outer bottom cardboard Remove the aluminum sheet wrapping the instrument box 5 Remove the instrument from the inner cardboard box The instrument is now in a...

Страница 12: ...p Door as described on page 6 to remove the platform stopper by removing the 3 screws using a small Philips head screwdriver 3 To remove the Styrofoam protector under the Syringe unit inside the iPrep...

Страница 13: ...urification Instrument is shown below Rear and Side View of iPrep Instrument The rear and side view showing various parts of the iPrep Purification Instrument are shown below Rear View Side View Conti...

Страница 14: ...o LEDs Power LED green indicates the power is ON and Alarm LED to indicate errors blinking red The Keypad to enter parameters and operate the instrument The keys described in the table below are for r...

Страница 15: ...ain menu Test menu and Manual menu The first line shows the current level The second and third line show the executable commands on this level The fourth line describes the keys to use for executing t...

Страница 16: ...r use with the iPrep Instrument are available separately from Invitrogen For more information visit www invitrogen com or contact Technical Support page 45 Product Quantity Catalog no iPrep Forensic C...

Страница 17: ...iction enzyme digestion See page 5 for details on various parts of the system System Components The iPrep Purification system consists of iPrep Purification Instrument The iPrep Purification Instrumen...

Страница 18: ...ontaining the purification reagents and the iPrep Tips and Tubes on the racks Place the assembled racks on the iPrep Platform 4 Select the purification parameters from the display to start the automat...

Страница 19: ...r of magnetic beads into the purified DNA Built in safety features in the instrument enhance user safety The ChargeSwitch Technology The ChargeSwitch Technology CST is a novel magnetic bead based tech...

Страница 20: ...solution Step 2 DNA Purification Any residual protein is digested using the protease and then washed away to leave pure intact DNA Step 3 Elution The pure DNA is then eluted into a small volume ready...

Страница 21: ...for a front and rear view of the device The front view of an open iPrep Purification Instrument identifying various parts is shown below Syringe Unit The Syringe Unit contains 13 nozzles that simulta...

Страница 22: ...ep Tip and Tube Rack allows easy placement of iPrep Tips Sample tubes with samples and Elution tubes to collect the eluate See page 8 for details User Interface The User Interface is located on the fr...

Страница 23: ...rd Do not wipe or clean the card using volatile chemicals such as alcohol or equivalent Do not expose the card to water or any solution Note If you accidentally damaged the iPrep Protocol Card you can...

Страница 24: ...ep Tip and Tube Rack The iPrep Tip and Tube Rack is a stainless steel rack designed to hold sample tubes tips in tip holders and elution tubes The rack dimensions are 11 3 inches l x 4 inches w x 3 in...

Страница 25: ...reader provides a positive reading feedback which includes an audible beep and a green spot on the barcode Each iPrep instrument includes 1 barcode reader 1 CD ROM with CommViewer Barcode Software and...

Страница 26: ...the protocol Cartridge Specifications Material Polypropylene cartridge sealed with laminated aluminum foil Max Volume 1000 l well Dimension 5 9 inches l x 1 2 inches w x 0 7 inches d iPrep Tips and Ho...

Страница 27: ...ation protocol The iPrep sample and elution tubes are included with each iPrep Kit and placed in their respective positions on the iPrep Tip and Tube Rack as described on page 26 Tube Specifications M...

Страница 28: ...s ChargeSwitch Magnetic beads are washed pH 7 0 Purified DNA is eluted from beads at pH 8 5 Materials Needed You will need the following items Ordering information is on page xvi iPrep Kits iPrep Prot...

Страница 29: ...ette butts 20 minutes iPrep ChargeSwitch Buccal Cell Kit Human buccal cell swabs 18 minutes iPrep GeneCatcher gDNA Blood Kit Human blood samples fresh whole blood EDTA or citrate anti coagulated blood...

Страница 30: ...ansfer the eluted sample to appropriate storage as described in the iPrep Kit manual Important You can load 1 13 iPrep Cartridges on the rack depending on the number of samples that you wish to proces...

Страница 31: ...o the AC inlet and then to the electrical outlet Use only properly grounded AC outlets and power cords You are ready to use the iPrep Instrument for nucleic acid purification See page 24 for the detai...

Страница 32: ...instrument ON Note To completely insert the card you need to manually push the card into the slot 5 Close the iPrep Card Slot 6 Using the Power Switch located on the left side of the instrument turn O...

Страница 33: ...ate 2 Time Key 1 2 ESC 5 The Date Screen is displayed Setup Sys Date Cur CMN CD CYYY Set SMN SD SYYY Key Up Dn RET ESC Row Curr displays the current month on CMN current date on CD and current year on...

Страница 34: ...esired date is displayed press Shift Down arrow to select the date and move the cursor to SYYY to display current year Note If the selected date exceeds the maximum date specified by SMN and SYYY then...

Страница 35: ...ed hour Once the desired hour is displayed move the cursor to SM to display the current minutes using Shift Down arrow Note 0 is the lower limit and 23 is the upper limit for the hour settings Setup S...

Страница 36: ...d below 1 Ensure the power switch is on the OFF position 2 Open the iPrep Card Slot 3 Push the button located below the card slot to eject the card from the slot 4 Pull out the card from the slot and...

Страница 37: ...need to use a commercially available USB to serial converter cable Connect the USB end of the cable into the USB port on the computer and connect the serial end of the cable into the PC port of the iP...

Страница 38: ...er InstallShield Wizard guides you through the installation process using the following steps Once the Welcome Window appears click Next This window allows you to select a folder to install the CommVi...

Страница 39: ...igital display on iPrep screen prompts you to Press 1 to output data On pressing 1 the barcode data read using the barcode reader appears in the Receive Data window 7 Select the Clear Receive Data to...

Страница 40: ...tion protocols are included in the manual supplied with the iPrep Kits or available for downloading from www invitrogen com Be sure the sample is prepared and ready for loading onto the instrument bef...

Страница 41: ...lick if the cartridge is placed correctly on the rack Important Do not just place the cartridge on the rack You need to slide the cartridge into the rack and push down Do not load reagent cartridges f...

Страница 42: ...in row marked as S Add the sample to each tube see the iPrep Kit manual for sample preparation details and sample volume recommendations The final assembly of iPrep Tip and Tube Rack is shown below 5...

Страница 43: ...ument to a computer using a RS232C serial cable to the PC port on the rear of the iPrep Instrument page 21 Install the barcode reader software on the computer as described on page 22 3 Insert the appr...

Страница 44: ...hrough the alphabet in case you wish to read letters The BS key is a backspace key that deletes the last digit character If you enter the sample number manually press Return to go to Screen 3 above Us...

Страница 45: ...er for that sample on the iPrep keypad For example to read the first elution tube barcode press 1 on the keypad Elution 1 1 Elution 2 2 Elution 3 3 Rtn Next Esc Prev 3 Read the barcode on the elution...

Страница 46: ...tus screens are displayed on the iPrep depending on whether the transmission was successful or rejected Successful Rejected Timeout Transmission Status Successful Press any key to continue Transmissio...

Страница 47: ...fication protocol begins and various steps of the protocol including the approximate time remaining are displayed on the digital display Important Do not open the door once the protocol has begun To p...

Страница 48: ...trical cord is accidentally removed from the outlet the purification protocol stops When the power resumes the digital display shows the main screen and the protocol cannot be continued An error is lo...

Страница 49: ...ves all axes to the original position Press 1 to go to the ORG display to move all axes to the origin if the tips do not need to be returned to the holders Once in the ORG screen you can individually...

Страница 50: ...t as described on page 16 2 Turn ON the instrument The Main screen is displayed 3 Press 3 to display the Test screen 4 The types of tests are displayed on the screen Axis test allows you to check move...

Страница 51: ...rrectly press Start to begin the test The duration of the test is 3 minutes During the Axis test all well hole positions are checked by moving tips on each position The display indicates the status of...

Страница 52: ...tically set to the preset value of 25 C To cool down the heater unit to this preset value turn off the instrument The temperature of the heater unit cools down at a rate of about 1 C per minute Do not...

Страница 53: ...pe Performed by Schedule Routine Cleaning of platform surface racks and bottom tray Maintaining the D ring Replacing the D Ring User User User After each use Bi weekly Annually Preventive Service Engi...

Страница 54: ...excess grease interferes with the operation of the instrument For any other repairs and service contact Technical Support page 45 Do not perform any repairs or service on the iPrep Instrument to avoi...

Страница 55: ...displayed on the digital display Error code displayed Make a note of the error code and see the list of error codes on page 42 If your specific error code is listed on the list on page 42 you need to...

Страница 56: ...ing the protocol To ensure proper mixing of reagents in the tip and prevent bubble formation during mixing make sure the sample volume is at least the recommended volume listed in the manual supplied...

Страница 57: ...y damaged Make sure that the beads are in solution at all times and do not dry Dried beads are non functional No DNA recovered Accidentally missed adding tubes or tips Be sure to add the sample and el...

Страница 58: ...ol in run 150 Invalid parentheses position 13 Failed to return to P axis protocol in run 151 Invalid big parentheses position 14 Failed to return to M axis protocol in run 160 Does not match variable...

Страница 59: ...perature 119 Function pointer buffer overflowed 306 Protocol failed to key in 120 No of character of data read is overflowed 307 Protocol failed to input variable 121 No of character of one command in...

Страница 60: ...chnical Support page 45 for a service engineer if the 6 3 A fuse needs replacement 1 Turn off the main power switch at the rear of the instrument and detach the power cord from the rear of the instrum...

Страница 61: ...te fax or email Additional international offices are listed on our Web page www invitrogen com Corporate Headquarters Invitrogen Corporation 1600 Faraday Avenue Carlsbad CA 92008 USA Tel 1 760 603 720...

Страница 62: ...not limited to 1 use of the product or its components in manufacturing 2 use of the product or its components to provide a service information or data 3 use of the product or its components for thera...

Страница 63: ...by the Purchaser This Warranty and the remedies set forth herein are exclusive and in lieu of all other express or implied warranties including implied warranties of merchantability fitness for a part...

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