ibidi Pump System
10
Troubleshooting
It is helpful to locate an issue with the ibidi Pump System and find a solution, by starting with the
failure description. Check the following subsections for the adequate failure description.
When contacting the
for assistance, please provide the relevant log files. The
log files are created automatically for every experiment, unless logging is deactivated. Standardly, the
logging interval is 30 seconds. The log files can be found in the PumpControl software by navigating
to ”Program”
”Program options...”
”program paths”. Activate the logging or define the logging
interval by going to ”Program”
”Program options...”
”general”.
10.1
Air Bubbles
Air bubbles in the tubing or the channel slide are a common issue in any type of perfusion setup. Air
bubbles can be avoided by following the precautions listed in this section.
10.1.1
Air Bubbles Emerging When Connecting the Slide
Issue:
Air bubbles emerge in the tubing system or slide directly after connecting the slide to the
Perfusion Set.
Possible Cause
Solution
Air bubbles were introduced while filling
the tubing (before connecting the channel
slide with cells).
To remove air bubbles in the tubing, start a cycle in
PumpControl with a high flow rate or load the “Re-
move air bubble” settings in the tutorial menu in the
software.
Air bubbles were introduced while con-
necting the Slide to the Perfusion Set.
Fill the slide reservoirs to the top and remove air bub-
bles on the liquid surface. When pulling out the Luer
adapter from the Female Luer Coupler, hold the male
Luer adapter upward so that the bubbles rise to the
female Luer coupler instead of flushing into the Luer
adapter. This procedure is shown in Section
10.1.2
Air Bubbles Emerging After a Few Hours
Issue:
Air bubbles emerge and accumulate somewhere in the tubing system after the experiment
has run for some time.
Possible Cause
Solution
Medium, tubing and slides were not de-
gassed and equilibrated at the proper
temperature.
Equilibrate the system parts inside the incubator one
day before starting the experiment (see Section
The humidity in the incubator is too
low. When working in a heated chamber
without humidification, evaporation pro-
motes air bubble formation.
Use an incubator with at least 80% humidity.
Version 2.6 (May 12, 2022)
72
Instruction Manual
