ibidi Heating System 4 Slides – Silver Line
2
Intended Use
The ibidi Heating System 4 Slides – Silver Line is a stage top incubator for live cell imaging that
fits onto inverted microscope stages with a mounting frame for multiwell plates. The ibidi Heating
System 4 Slides, consisting of an Incubation Chamber with Heated Plate and Heated Lid and a Tem-
perature Controller, provides a platform for keeping ibidi µ-Slides at a constant temperature during
an experiment on a microscope.
For full control of the incubation environment, the ibidi Heating System 4 Slides can be combined
with the ibidi Gas Incubation System to regulate the CO
2
and O
2
concentrations as well as the hu-
midity.
The ibidi Heating System 4 Slides is for research use only! It is not intended for use in diagnostic
procedures.
3
Principle
Physiological Conditions in Live Cell Imaging
Cells react sensitively to changes in their environment. Factors such as temperature, humidity, and
CO
2
/O
2
levels significantly influence the outcome of cell culture assays. In order to achieve biologi-
cally relevant and reproducible results, it is crucial to maintain optimal conditions on the microscope
stage during live cell imaging experiments.
To enable high-quality live cell imaging under physiological conditions, the following parameters
need to be considered:
Environment:
• Temperature
• Carbon Dioxide (CO
2
) Levels and pH of the
Medium
• Humidity and Evaporation
• Oxygen (O
2
) Levels
Optical Aspects:
• Condensation
• Imaging Method and Photon Dose
Figure 1: Live cell imaging conditions.
The Gas Incubation System provides a controlled atmosphere of gas (CO
2
or CO
2
/O
2
) as well as
a defined humidity in the emitted gas stream that is flushed continuously through the incubation
chamber. Oxygen can be reduced during the experiment, enabling the user to perform hypoxia
experiments (CO
2
/O
2
version only).
Version 1.0 (August 1, 2022)
14
Instruction Manual
