ibidi 10929, Инструкция по эксплуатации

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ibidi Heating System, Multi-Well Plates, K-Frame
2 Intended Use
The ibidi Heating System, Multi-Well Plates, K-Frame is a stage top incubator for live cell imaging,
that fits onto microscope stages with a mounting frame K opening (110 × 160 mm). The geometry of
the chamber with a heated glass plate underneath and also above the sample, provides a platform
for keeping multi-well plates at a constant temperature during the experiment on the microscope.
For full control of the incubation environment, the ibidi Heating System, Multi-Well Plates, K-Frame
can be combined with the ibidi Gas Incubation System to regulate the CO
2
and O
2
concentrations as
well as the humidity.
3 Principle
The Heated Lid and Heated Plate with the Heated Glass Bottom are designed to keep cells on–stage
in a multiwell plate at 37°C. The Heated Plate is mounted on the microscope stage. The multiwell
plate is placed directly on the Heated Glass Bottom and the Heated Lid is put on top.
The gas mixture is streamed into the chamber through the gas inlet and then slowly escapes through
the gaps between the Heated Lid, Heated Plate, and Heated Glass Bottom (small gray arrows in
Figure
1 ).
Figure 1 – Schematic cross-sectional view of the ibidi Heating System, Multi-Well Plates, K-Frame.
The Heated Lid prevents condensation effects inside the entire incubation system (Figure 2 ).
Figure 2 – The Heated Lid prevents condensation effects on the lid of the cell culture vessel.
Instruction Manual 13 Version 2.0 (March 22, 2019)