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Standard Operations
5.7.1.2 Take single wavelength measurements (single reading)
1.
Insert the blank cuvette/cell into the cuvette/sample cell holder.
Press
Zero
.
Note:
The
Read
key is only active after the zero measurement has
been completed.
2.
Insert the sample cuvette/cell into the cuvette/sample cell
holder. Press
Read
.
3.
For data storage, see
.
5.7.1.3 Take single wavelength measurements (continuous readings)
1.
Insert the blank cuvette/cell into the cuvette/sample cell holder.
Press
Zero
.
Note:
In the reading mode ”Continuous“ only the Zero key is shown to
start the reading. The reading sequence is started automatically.
2.
Insert the sample cuvette/cell into the cuvette/sample cell
holder.
3.
Press
Options
and then the
Store
icon to store the displayed
data in the Data Log.
Note:
5.7.2 Multi-Wavelength mode – measurements at more than one wavelength
In the multi-wavelength mode, absorbance values can be
measured at up to four wavelengths and the results can be
mathematically processed to obtain sums, differences and
relationships.
Absorbance measures the amount of light absorbed by the sample,
in units of Absorbance.
% Transmittance measures the percent of the original light that
passes through the sample and reaches the detector.
Turning on the concentration factor allows selection of a specific
multiplier for converting absorbance readings to concentration. In a
graph of concentration versus the absorbance, the concentration
factor is the slope of the line. Concentration is calculated using a
single factor for each wavelength, which is input by the user.
5.7.2.1 Set the reading mode at different wavelengths
Press
Multi Wavelength
in the Main Menu. Press
Options
for
Parameter Setup.
describes the multi
wavelength setup options.