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60
Advanced Operations
6.3 Single Wavelength Mode
The Single Wavelength Mode can be used in three ways. For
sample measurements at a single wavelength, the instrument can
be programmed to measure the absorbance, % transmittance, or
concentration of the analyte.
6.3.1 Setting Up Single Wavelength Mode
1. From the Main Menu, press
SINGLE WAVELENGTH
.
2. Press
OPTIONS
for Parameter Setup.
Table 9 Single Wavelength Setup Options
Option Description
Store Off/On
With
STORE ON
selected, all measurement data are stored automatically. With
STORE OFF
selected, no measurement data are stored.
% Trans/Abs/Conc
Toggles between % transmittance, absorbance readings, or concentration
Absorbance: Measures the amount of light absorbed by the sample, in units of Absorbance.
% Transmittance : Measures the percent of the original light that passes through the sample and
reaches the detector.
Concentration: Allows the selection of a specific multiplier for converting absorbance readings
to concentration.
λ
(Wavelength)
Enters the measurement wavelength. Use the alphanumeric keypad to enter the measurement
wavelength. The entered wavelength must be in the range from 340–900 nm.
Timer icon
Functions as a stopwatch. Ensures that the steps of an analysis are correctly timed (e.g. reaction
times, wait times, etc., can be exactly specified). When the specified time has elapsed, an audible
signal is emitted. The timer has no influence on the measurement program.
Concentration Factor
Multiplication factor for converting absorbance values into concentration values. In a graph of
concentration versus the absorbance, the concentration factor is the slope of the line.
Concentration Resolution Select the position of the decimal point in the calculated concentration readings.
Save as User Program Stores the selected parameters as a User Program ( section 6.1 on page 47 )
Recall Data Recalls saved measurement data, wavelength scans, or time courses ( section 5.3 on page 28 ).
Instrument Setup Basic operation settings of the instrument ( section 5.2 on page 23 ).