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4.
Add the contents
of 1 nutrient buffer
pillow to the
graduated cylinder.
5.
Move the
contents of the
graduated cylinder to
a BODTrak II bottle.
Continue to
Completion steps for
all procedures
on page 12. Repeat
steps 1 to 5 for
additional samples.
7.3 Hach Standard Method procedure
Items to collect:
• Thermometer
• BODTrak II bottle
• Blender (optional)
• Graduated cylinder
• Tensette pipet and pipet tips
• Wash water bottle
• Ampule breaker (for GGA)
Required reagents:
• 1 nutrient buffer pillow
• Additional nutrient or buffer (optional)
• Deionized water
• Hach GGA Solution (optional)
Before starting the test:
Use the sample volume table to select the correct sample size.
If samples are seeded, prepare a seed blank before a sample is
prepared. Treat the seed blank the same as any other sample and
omit step 5.
Use Hach BOD Standard Solution Ampules for Manometric Method
(3000 mg/L Glucose, 3000 mg/L Glutamic Acid) for GGA.
on page 15 for special
considerations.
Use the correct volumes and dilution factor for the BOD range as shown
in
Table 5 Hach Standard Method sample volumes
BOD range
(mg/L)
Sample
volume
(mL)
Seed
volume (mL)
Final
volume
(mL)
Dilution
factor
0 to 35
370
10 to 35
420
1.14
0 to 70
305
10 to 35
355
1.16
0 to 350
110
10 to 35
160
1.45
0 to 700
45
10 to 35
95
2.11
GGA
(0 to 350)
8
10 to 35
160
—
3
3
The GGA result calculation does not include a dilution factor. Refer to
on page 14, step
result.
English
11
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