7
pH Calibration
The pH100A /pH100M uses a 2-point calibration. The first point must be a
6.86/7.00 buffer, and the second either a 4.00/4.01 or 9.18/10.01.
1. Turn the unit on. Connect the pH electrode to the BNC connector and
the ATC/Temp probe to the ATC/Temp connector of the unit; “ATC”
displays. Press
Mode
until “pH” displays. AUTOLOCK may be on or off
as desired.
2. Place the pH and ATC/temp probes into the first buffer solution (either
7.00 or 6.86). Allow temperature readings to stabilize, then press and
hold
Stand/Recal
for 3 seconds to calibrate. If AUTOLOCK is off, the
first point has been calibrated. If AUTOLOCK is on, “WAIT” flashes
until the unit detects a stable reading. Once the unit calibrates the first
point “SLOPE” flashes.
Note: If no temperature probe is connected, adjust the temperature
reading to that of the first buffer using the up or down arrow keys
(0.0 to 60 ºC) BEFORE pressing
Stand/Recal
.
3. Rinse the pH and ATC/temp probes in distilled water, then place into
the second buffer solution (either 4.01/4.00 or 10.01/9.18). Allow
temperature readings to stabilize, then press
Slope/
YSI EcoSense Handheld Overlay
1/26/2016
pH100M
colors:
Black background
PMS 7482 C power symbol
PMS 130 C logo
Reverse white all other imagery
pH
100
M
ATC
EcoSense
®
pH
Mode
Stand
Slope
Effic.
ESC
Recal
Meas.
to calibrate. If
AUTOLOCK is off, the second point has been calibrated. If AUTOLOCK
is on, “WAIT” flashes until the unit detects a stable reading. Once the
unit calibrates the second point, the unit beeps once and both
“STAND” and “SLOPE” display steadily.
Note: If no temperature probe is connected, adjust the temperature
reading to that of the second buffer using the up or down arrow
keys (0.0 to 60 ºC) BEFORE pressing
Slope/
YSI EcoSense Handheld Overlay
1/26/2016
pH100M
colors:
Black background
PMS 7482 C power symbol
PMS 130 C logo
Reverse white all other imagery
pH
100
M
ATC
EcoSense
®
pH
Mode
Stand
Slope
Effic.
ESC
Recal
Meas.
.
4. The unit calculates and compensates for the pH electrode slope
deviation corresponding to the values of the two calibration buffers.
The unit is now dual-point calibrated and ready for measurements.
After calibration, press and hold
Meas./Effic.
for about 5 seconds to
display the new electrode efficiency.
Using the model pH160 Electrode Simulator
The model pH160 Electrode Simulator can be used to confirm proper
instrument calibration. To use the simulator:
1. Install the 9V battery provided.
2. Attach the pH160 to the pH100A. Turn both units on. The pH160 has a
small switch to the right of the pH buttons.
3. In pH measurement mode, press one of the pH buffer simulator
buttons on the pH160. The corresponding pH value should appear on
both screens.
