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7
1.4
Safety & Maintenance
To ensure optimal performance, we recommend regularly cleaning the eZwest
instrument.
Component Description
eZwest
device:
routine
cleaning
After each use, please clean the device following the
procedures below.
1. Insert the membrane cassette into the device.
2. Select “system clean” method
3. Prepare 30mL wash buffer (PBST or TBST) in a 50 mL tube
and place it in the Blocking buffer slot. Add distilled water in
the 1° Ab and 2° Ab tubes. Click OK
4. Ensure there is at least 100 ml buffer in the Wash buffer
bottle and the Waste bottle is empty.
5. Start “System Clean” method by clicking OK again.
6. After method finishes, remove the membrane cassette.
eZwest
device:
extensive
cleaning
After every 20 use or every 2 weeks
,
perform the extensive
cleaning following the procedures below.
1. Empty the Wash bottle and Waste bottle
2. Add 100 mL deionized water in Wash bottle
3. Insert the cleaning cassette into the device.
4. Select “system clean” method
5. When prompted, prepare 30ml of eZwest cleaning solution
(Appendix I) in 50 mL tube and place it in the Blocking buffer
slot. Add distilled water in the 1° Ab and 2° Ab tubes. Click OK.
6. Start “system clean” grogram.
7. After method is finished, dispose the solution in Waste
bottle.
8. Perform the routine cleaning protocol once.
Membrane
cassette
Open the membrane cassette and rinse with distilled water or
10% ethanol and let it dry after each use.
Note
If the device will be left unused for a long period of time.
Please ensure pipelines in machine is filled with Regular wash
buffer.
Содержание eZwest Lite
Страница 1: ...Automated Western Device...
Страница 2: ......
Страница 4: ...2...
Страница 11: ...9 Fig 3 Top view Fig 4 Membrane cassette Fig 5 Display main menu...
Страница 25: ...23 1x PBST buffer Recipe 10 PBS 100mL Tween20 0 5mL Add water 900mL Mix thoroughly pH should be around 7 4...
Страница 26: ...24...
Страница 27: ......