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14
Set cassette specific method
The eZwest Lite device can store up to four dedicated methods for each
cassette. Upon cassette installation, select the associated method.
1.
Method settings can be changed as described above
2.
Change name of membrane cassette
a.
Under parameter interface, select the name of membrane cassette
Fig.16
b.
Press “Edit ID” and change name with the pop-up keyboard.
Fig.17
2.3
Using the Pre-programmed blotting method
1.
Prepare blotting solutions
a.
Blocking buffer, 10 mL
b.
Primary antibody solution, 10 mL
c.
Secondary antibody solution, 10 mL
d.
Wash buffer, 10 mL per wash cycle, e.g. 90 mL if wash 3 times
and each with 3 cycles
Содержание eZwest Lite
Страница 1: ...Automated Western Device...
Страница 2: ......
Страница 4: ...2...
Страница 11: ...9 Fig 3 Top view Fig 4 Membrane cassette Fig 5 Display main menu...
Страница 25: ...23 1x PBST buffer Recipe 10 PBS 100mL Tween20 0 5mL Add water 900mL Mix thoroughly pH should be around 7 4...
Страница 26: ...24...
Страница 27: ......