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Version 2.0

June 4, 2010

Protein Staining System

User Manual

For electric staining of proteins in mini polyacrylamide gels

The Biology CRO

GenScript USA Inc.

120 Centennial Ave.

Piscataway, NJ 08854

Tel: 732-885-9188, 732-885-9688

Fax: 732-210-0262, 732-885-5878

Email: [email protected]

Содержание eStain

Страница 1: ...Version 2 0 June 4 2010 Protein Staining System User Manual For electric staining of proteins in mini polyacrylamide gels The Biology CRO...

Страница 2: ...fications eStainTM Protein Staining Device Accessory Products Introduction Quick Reference Guide Protocols Troubleshooting Examples of Results Technical Support Warranty 1 3 5 7 8 11 12 21 22 23 24 eS...

Страница 3: ...inTM Protein Staining Pads G 250 20 pak Cat No L02011 L02012 This manual is supplied with the following products The contents of the eStainTM Protein Staining Device are listed below Examine the unit...

Страница 4: ...ainTM Protein Staining Pads R 250 20 pak contains eStainTM Protein Staining Pad R 250 Gel Window 66mm 56mm Gel Window 76mm 64mm The eStainTM Protein Staining Pads G 250 20 pak contains eStainTM Protei...

Страница 5: ...Staining Pads Specifications size Materials 8cm l x 7cm w x2 5mm thick Blotting filter paper presoaked with proprietary cathode buffer containing CBB R 250 or G 250 size Materials 8cm l x 7cm w x2 5m...

Страница 6: ...1mm h 180g Powdered carbon clay eStainTM Graphite Electrode 11mm The eStainTM Graphite Electrode is used as the replaceable anode of eStainTM Protein Staining Device and available separately from GenS...

Страница 7: ...ce 5 The rear view showing various parts of the eStainTM Protein Staining Device is shown below The front top view showing various parts of the eStainTM Protein Staining Device is shown below Control...

Страница 8: ...are on which indicates numbering mode the Digital Display shows how many times of electric staining the eStainTM Graphite Electrode has been used for The Reset button is used to clear parameters The...

Страница 9: ...TM PAGE Gels as well as premade buffers or buffer powders are available from GenScript For details contact Technical Support or visit www genscript com eStainTM Protein Staining Pads are available sep...

Страница 10: ...nal Coomassie blue staining method into a single step and greatly cuts down the time required for protein staining analysis The proteins stained using the eStainTM Protein Staining System exhibit as h...

Страница 11: ...gatively charged staining reagents The high electric force generated between graphite anode and titanium cathode allows for rapid and directional movement of negatively charged Coomassie blue dye reag...

Страница 12: ...owing for easy and convenient procedures Consumable staining pads offering convenience without the need for additional solutions and buffers High staining efficiency as conventional Coomassie blue sta...

Страница 13: ...d running time Press Start Stop button Automatic Press and hold Time button for 2 seconds Switch off the device and replace the worn graphite anode with a new one Sound Continuous beeping for 2 minute...

Страница 14: ...ed AC outlets and power cords 6 When the electrophoresis of your samples is almost complete press the power switch located on the rear of the device to turn ON the eStainTM Protein Staining Device The...

Страница 15: ...ition then push it forward to open the lid of eStainTM Device 2 Remove one package labeled as eStainTM Protein Staining Pad from the eStainTM Protein Staining Pads box and tear the laminated sealing o...

Страница 16: ...te and rinse the gel with 50 to 100 ml distilled water for about 1 minute 5 Place the gel on the eStainTM Anode Pad Gently remove air bubbles between gel and anode pad using the small shovel supplied...

Страница 17: ...The edges of the pre run gel may also be covered by Gel Window and will probably be stained to deep blue after running an electric staining program 6 Select appropriate Gel Window according to actual...

Страница 18: ...taining stack perform electric staining as described below 1 Press the Time button to set appropriate running time based on the gel thickness see table below If an undesired running time is set by mis...

Страница 19: ...ein Staining Pad Note Do not re use the eStainTM Protein Staining Pad after staining Discard after each use 4 Clean the titanium cathode plate graphite anode plate and its surrounding area with a dry...

Страница 20: ...bering mode If the Digital Display shows 200 or a number greater than 200 perform the replacing protocol as describe below Protocols continued During electric staining process the eStainTM Graphite El...

Страница 21: ...ge and take the new Graphite Electrode out of the package Place the new Graphite Electrode into the anode tank and close the lid of the device 5 Switch On the eStainTM Device Press and hold Time butto...

Страница 22: ...inTM Device you are ready to use the eStainTM Device for staining applications again 6 Press Reset button to zero the staining times Note For the best results after using Graphite Electrode per 40 tim...

Страница 23: ...les present in staining stack interfering gel staining Solution Ensure the staining stack is assembled correctly use the eStainTM Anode Pad first followed by the gel Gel Window and eStainTM Cathode Pa...

Страница 24: ...les were separated on a 12 Tris Glycine gel and stained using eStainTM Protein Staining System R 250 Tris Glycine gel results Bis Tris gel results 1 2 3 4 5 6 7 8 Lane 1 500ng rabbit IgG Lane 2 10 l E...

Страница 25: ...s formulations citations handbooks etc 2 Complete technical support contact information 3 Access to the GenScript Online Catalog 4 Additional product information and special offers For more informatio...

Страница 26: ...2 Repair or modification done by anyone other than GenScript or an authorized agent 3 Use of fittings or other spare parts supplied by anyone other than GenScript 4 Damage caused by accident or misuse...

Страница 27: ...GenScript USA Inc 120 Centennial Ave Piscataway NJ 08854 Tel 732 885 9188 732 885 9688 Fax 732 210 0262 732 885 5878 Email estain genscript com...

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