- 10 -
Introduction,
continued
Introduction,
continued
e
Blot
TM
Protein Transfer System
www.genscript.com
System Features
- 9 -
e
Blot
TM
Protein Transfer System
www.genscript.com
Important features of the
e
Blot
TM
Protein Transfer System are listed below:
·
Unique semi-dry electroblotting technique created for fast, reliable protein
transfer within 7-10 minutes.
·
Self-contained electroblotting unit with an integrated power supply for easy
and convenient procedures.
·
Consumable transfer pads offering convenience without the need for
additional buffers.
·
Compatible for use with various types of mini polyacrylamide gels.
·
Proprietary formula without methanol .
·
High transfer efficiency as compared to other existing blotting methods.
System Mechanism
The
e
Blot
TM
Protein Transfer System is based on the proprietary fast semi-dry
blotting technique developed by GenScript.
To use the
e
Blot
TM
Protein Transfer System for rapid protein blotting, assemble
the
e
Blot
TM
Protein Transfer Pad with your pre-run gel and the membrane on
the
e
Blot
TM
Protein Transfer Device. The
e
Blot
TM
Cathode Pad and
e
Blot
TM
Anode Pad act as ion reservoirs that contain the appropriate anode and
cathode buffers. The design of the
e
Blot
TM
Protein Transfer Device reduces
the distance between electrodes and the integrated power supply enables the
system to generate a certain definite voltage allowing for rapid and directional
movement of negatively charged protein molecules from the gel matrix onto
the membrane within 7-10 minutes. Meanwhile, the membrane presoaked with
e
Blot
TM
Equilibration Buffer shows good combination ability with proteins that
prevents the flow through of low molecular weight proteins from the membrane.
Schematic mechanism of
e
Blot
TM
Protein Transfer System showing the flow
of current:
TM
TM
Window
