GenScript eBlot Скачать руководство пользователя страница 6

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Accessory Products

Introduction

e

Blot

TM

 Protein Transfer System 

www.genscript.com

Precast Gels and Premade 

Buffers

ONE-HOUR Western

TM

 

Detection Kits

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e

Blot

TM

 Protein Transfer System 

www.genscript.com

System Overview

System Components

Semi-dry Western blotting is a common technique applied in protein research. 

Conventional semi-dry blotting is a cumbersome process, requiring time

-consuming reagent preparation and setup, followed by an electrophoretic 

transfer that could take one hour or more. GenScript’s 

e

Blot

TM

 Protein Transfer

System accelerates the semi-dry blotting process without sacrificing performance.

The 

e

Blot

TM

 Protein Transfer System, consisting of the 

e

Blot

TM

 Protein Transfer 

Device and 

e

Blot

TM

 Protein Transfer Pads, enables researchers to quickly, 

reliably perform electrophoretic transfer of proteins from various types of mini 

polyacrylamide gels to membranes in 7 to 10 minutes without the need to 

prepare additional buffers.The proteins transferred using the 

e

Blot

TM

 Protein 

Transfer System exhibit high detection sensitivity as to proteins transferred 

using other existing blotting methods. 

The 

e

Blot

TM

 Protein Transfer System consists of: 

eBlot

TM

 Protein Transfer Device

The 

e

Blot

TM

 Protein Transfer Device is a self-contained electroblotting unit 

with a power supply built-in that allows for fast transfer of proteins.

eBlot

TM

 Protein Transfer Pads

As the consumable part of 

e

Blot

TM

 Protein Transfer System, the 

e

Blot

TM

Protein Transfer Pads are the mixed assortment of 

e

Blot

TM

 Protein Transfer

Pad, nitrocellulose or PVDF membranes, Equilibration Buffer and Gel 

Window. Each pack of 

e

Blot

TM

 Protein Transfer Pad contains an 1×

e

Blot

TM

 

Cathode Pad and an 1×

e

Blot

TM

 Anode Pad presoaked with proprietary 

cathode buffer and anode buffer respectively, allowing for rapid, convenient 

and reliable protein blotting without the need to prepare additional buffers. 

The ONE-HOUR Western

TM

 Detection Kits used for Western blotting analysis 

are available from GenScript. Ordering information is provided below. For

more information, visit 

www.genscript.com 

or call Technical Support.

The precast Express

TM

 PAGE Gels as well as premade buffers and buffer 

powders are available from GenScript. For details, contact Technical 

Support or visit 

www.genscript.com.

Product

ONE-HOUR Western

TM

 Basic Kit (Rabbit)

ONE-HOUR Western

TM

 Basic Kit (Mouse)

ONE-HOUR Western

TM

 Basic Kit (Goat)

ONE-HOUR Western

TM

 Standard Kit (Rabbit)

ONE-HOUR Western

TM

 Standard Kit (Mouse)

ONE-HOUR Western

TM

 Standard Kit (Goat)

ONE-HOUR Western

TM

 Standard Kit with TMB (Rabbit)

ONE-HOUR Western

TM

 Standard Kit with TMB (Mouse)

ONE-HOUR Western

TM

 Standard Kit with TMB (Goat)

ONE-HOUR Western

TM

 Advanced Kit (Rabbit)

ONE-HOUR Western

TM

 AdvancedKit (Mouse)

ONE-HOUR Western

TM

 Advanced Kit (Goat)

ONE-HOUR IP-Western Kit (Rabbit)

ONE-HOUR IP-Western Kit (Mouse)

ONE-HOUR IP-Western Kit (Goat)

ONE-HOUR Western

TM

 Fluorescent Kit

ONE-HOUR Western

TM

 Multiplex Fluorescent Kit

Cat.  No.

L00204

L00205

L00399

L00204C

L00205C

L00228

L00204T

L00205T

L00228T

L00241

L00242

L00243

L00231

L00232

L00233

L00397

L00398

Quantity

1 Kit (5 Assays)

1 Kit (5 Assays)

1 Kit (5 Assays)

1 Kit (5 Assays)

1 Kit (5 Assays)

1 Kit (5 Assays)

1 Kit (5 Assays)

1 Kit (5 Assays)

1 Kit (5 Assays)

1 Kit (5 Assays)

1 Kit (5 Assays)

1 Kit (5 Assays)

1 Kit (5 Assays)

1 Kit (5 Assays)

1 kit (5 Assays)

1 Kit (10 Assays)

1 Kit (10 Assays)

Содержание eBlot

Страница 1: ...iscataway NJ 08854 Tel 732 885 9188 732 885 9688 Fax 732 210 0262 732 885 5878 Email product genscript com Version No 08152014 For fast semi dry electroblotting of proteins from mini polyacrylamide ge...

Страница 2: ...ions eBlotTM Protein Transfer Device Accessory Products Introduction Quick Reference Guide Protocols Troubleshooting Examples of Results Technical Support Warranty Appendix 1 3 5 7 8 11 12 21 22 23 24...

Страница 3: ...mponent eBlotTM Protein Transfer Pad Nitrocellulose membranes 20 sheets PVDF membranes 20 sheets eBlotTM Equilibration Buffer 125 ml Gel Window 66 mm 56 mm Gel Window 76 mm 64 mm Gel Window 88 mm 78 m...

Страница 4: ...ely from GenScript The specifications for eBlotTM Graphite Electrode are listed below eBlotTM Protein Transfer Pads Specifications eBlotTM Anode Pad eBlotTM Cathode Pad Materials 90 mm l 80 mm w 2 5 m...

Страница 5: ...both left and right status lights are on the device is working at numbering mode The Reset button is used to clear parameters The Min button is used to shift between transferring and numbering mode a...

Страница 6: ...s Equilibration Buffer and Gel Window Each pack of eBlotTM Protein Transfer Pad contains an 1 eBlotTM Cathode Pad and an 1 eBlotTM Anode Pad presoaked with proprietary cathode buffer and anode buffer...

Страница 7: ...etary fast semi dry blotting technique developed by GenScript To use the eBlotTM Protein Transfer System for rapid protein blotting assemble the eBlotTM Protein Transfer Pad with your pre run gel and...

Страница 8: ...ng the number of uses of the graphite anode Replacement of worn graphite anode Action Connect eBlotTM Device to an electrical outlet and power switch is on Assemble transfer stack on the device and cl...

Страница 9: ...e Protocols continued Protocols continued 8 Attach the power cord to the AC inlet and then to the electrical outlet Use only properly grounded AC outlets and power cords 9 When the electrophoresis of...

Страница 10: ...nd cathode pad to prevent short circuit Note During assembling of the transfer stack make sure to remove all the air bubbles trapped between the transfer pads pre run gel and the membrane which may pr...

Страница 11: ...o perform the replacement Note After about 40 runs take out the graphite electrode soak in distilled water for 30 minutes dry the surface with a clean paper towel then leave air dry overnight with gra...

Страница 12: ...phite Electrode out of the device 3 Tear the sealing of a new Graphite Electrode package and take the new Graphite Electrode out of the package Place the new Graphite Electrode into the anode tank and...

Страница 13: ...ansfer stack is assembled correctly use the eBlotTM Anode Pad first followed by the membrane the pre run gel Gel Window and eBlotTM Cathode Pad Check the transfer stack and ensure Gel Window covered c...

Страница 14: ...vent or sample For any inquiry or request for repair service contact GenScript after confirming the model and serial number of your instrument For your protection items being returned must be insured...

Страница 15: ...26 25 Appendix Appendix continued eBlotTM Protein Transfer System www genscript com eBlotTM Protein Transfer System www genscript com...

Страница 16: ...27 Appendix continued eBlotTM Protein Transfer System www genscript com...

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