GenScript eBlot, Руководство пользователя

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Protocols,
continued
Protocols,
continued
e Blot TM Protein Transfer System www.genscript.com
Assembling the Transfer
Stack, continued
Assembling the Transfer
Stack, continued
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e Blot TM Protein Transfer System www.genscript.com
8. Place the gel on the membrane. Gently remove air bubbles between the
gel and the membrane.
9. Select appropriate Gel Window according to actual size of the gel (see
the table below). Place Gel Window on the gel. Ensure that the Gel
Window fully covers the margin of the membrane.
10. Tear the sealing of the 1× e Blot TM Cathode Pad package. Remove the
e Blot TM Cathode Pad from the package and place it on the gel.
Note: Gel Window is used as the spacer between anode pad and cathode pad to
prevent short circuit.
Note: During assembling of the transfer stack, make sure to remove all the air bubbles
trapped between the transfer pads, pre-run gel and the membrane, which may
prevent the transfer of proteins and cause empty spots on the transferred membrane.
6. Place the equilibrated membrane on the e Blot TM Anode Pad. Gently remove
air bubbles between the membrane and the anode pad using the small
shovel supplied with the device.
7. Carefully remove the pre-run gel containing your protein samples from the
gel cassette and briefly rinse the gel with distilled water in another shallow
tray to remove any small gel pieces attached to the gel and facilitate easy
positioning of the gel on the membrane.
Pre-run gel
Gel with size > 80 × 60 mm
Gel with size 66 × 56 mm to 80 × 60 mm
Gel with size < 66 × 56 mm
Gel Window
Gel Window 88 × 78 mm
Gel Window 76 × 64 mm
Gel Window 66 × 56 mm