GE MicroCal iTC200, Руководство по эксплуатации

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MicroCal iTC
200
Operating Instructions 28-9639-77 AA
41
Operation 4
Loading the cell 4.4
4
Pull approximately 300 µl of sample into the syringe, and tap the syringe glass so
that all air is at the top volume of the syringe. Do not allow air to be put into the
cells.
5
After removing the bubbles, insert the syringe into the cell and gently touch the
bottom of the cell with the tip of the syringe needle.
6 Raise the needle tip about 1 mm off the bottom of the cell, and hold it there until
finished filling. Do not raise the syringe during the filling process.
7
Slowly inject solution into the cell until it spills out the top of the cell stem. Finish the
filling with several small abrupt spurts of solution to dislodge any bubbles in the
cells.
8
Finally, lift the tip of the syringe to the cell port (just below the visible portion of the
cell port) and find the ledge (See
Figure 4-3 ) that is formed where the cell stem
meets the cell port. Place the syringe on the ledge at the top of the metal cell stem
and remove the excess solution. If the reference cell needs refilling, follow the same
procedure as for the sample cell.
Figure 4-3. Schematic representation of the cell (left) and the top of the cell stem (right) where air
bubbles can form if not loaded properly.
Load the run parameters by clicking the Load Run File button at the top left of the
Advanced Experimental Design tab in the MicroCal iTC
200
software and select the
WATER.inj file. This will populate all the required fields for performing a run. Then click
the Start button on the bar of buttons across the top of the control software.
Part Description
1 Place where bubbles are trapped.
2 Ledge formed where cell stem meets plastic overflow reservoir.
1
2