5.5
After the transfer is complete
Introduction
This section describes how to remove the gel from the transfer stack and how to turn
off PWR LARGE SEMIPHOR TRANSPHOR UNIT and PWR SEMIPHOR TRANSPHOR UNIT.
After a transfer with LARGE
SEMIPHOR TRANSPHOR UNIT and
SEMIPHOR TRANSPHOR UNIT
After the transfer is completed, turn off the power supply and disconnect the leads from
the power supply.
After a transfer with PWR LARGE
SEMIPHOR TRANSPHOR UNIT and
PWR SEMIPHOR TRANSPHOR
UNIT
After a transfer is complete, the unit will beep for 5 seconds. The LED display will blink
and cycle between the values for the current, time and voltage at the time the transfer
was stopped. These can be recorded into a notebook, if desired. Pushing any button will
clear the display.
Remove gel from transfer stack
Follow the instructions below to take the gel and the membrane out of the transfer stack.
Action
Step
Remove the cover slowly because the stack may adhere to it.
1
Remove and dispose of the upper blot papers.
2
Remove the gel(s).
3
Optional: Stain the gel to check for residual protein that may be left in the
gel.
Note:
Staining the gel(s) for residual protein gives an indication of the completeness
of transfer.
4
52
SEMIPHOR TRANSPHOR UNIT series Operating Instructions 29281777 AA
5 Operation
5.5 After the transfer is complete
