Buffer depletion
One of the more common problems for a semi-dry electrotransfer is buffer depletion.
Buffer depletion leads to changes in pH and overheating, both of which are detrimental
to the transfer.
PWR LARGE SEMIPHOR TRANSPHOR UNIT and PWR SEMIPHOR TRANSPHOR UNIT monitor
the transfer stack resistance. Large changes in resistance indicate the depletion of the
buffer system. The instrument will stop the transfer before the stack resistance becomes
too high and lead to further problems, like burning of the transfer stack.
Follow the instructions below to add buffer when the buffer is depleted during the elec-
trotransfer.
Action
Step
Check if the display shows the error message dry, as shown in the illustration
below.
1
Note:
The instrument has stopped the transfer.
Remove the cover slowly because the stack may adhere to it.
2
Add buffer to the transfer stack.
3
Replace the cover, and press any key except Power ON/OFF to continue.
Note:
For future transfers, add more layers of buffer soaked blot paper.
4
SEMIPHOR TRANSPHOR UNIT series Operating Instructions 29281777 AA
51
5 Operation
5.4 Changing parameters during the transfer
