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Electrotransfer with LARGE
SEMIPHOR TRANSPHOR UNIT and
SEMIPHOR TRANSPHOR UNIT

Follow the instructions below to start an electrotransfer with the LARGE SEMIPHOR
TRANSPHOR UNIT and SEMIPHOR TRANSPHOR UNIT.

Action

Step

Make sure that the power supply is switched OFF.

1

Plug the color-coded leads from the base of the transfer unit into the power
supply jacks, matching red to red and black to black. Do not reverse polarity.
Red is positive (+) and black is negative (–).

2

Set the voltage limit to 30 V maximum. The input voltage must not exceed
30 V or the protection will be activated.

3

Make sure that the maximum current does not exceed 0.8 mA/cm

2

of the

gel surface or 500 mA maximum.

4

If available, set time for the transfer on the power supply.

5

Most transfers are complete within one hour, but larger proteins, proteins
from native gels, thicker gels and transferring several layers of gels, may
require an additional 1 hour of transfer time. The optimum transfer time for
each protein and gel system must be determined empirically.

Note:

Generally, smaller fragments transfer more quickly than larger ones.

Add sheets of buffer saturated blot paper in the transfer stack if the transfer
run time exceeds one hour.

6

46

SEMIPHOR TRANSPHOR UNIT series Operating Instructions 29281777 AA

5 Operation

5.2 Electrotransfer with LARGE SEMIPHOR TRANSPHOR UNIT and SEMIPHOR TRANSPHOR UNIT

Содержание LARGE SEMIPHOR TRANSPHOR UNIT

Страница 1: ...SEMIPHOR TRANSPHOR series Operating Instructions Original instructions ...

Страница 2: ...ription 29 3 1 Instrument overview 31 3 2 Detailed description 34 4 Installation 35 5 Operation 37 5 1 Assemble transfer stack 45 5 2 Electrotransfer with LARGE SEMIPHOR TRANSPHOR UNIT and SEMIPHOR TRANSPHOR UNIT 47 5 3 Electrotransfer with PWR LARGE SEMIPHOR TRANSPHOR UNIT and PWR SEMIPHOR TRANSPHOR UNIT 50 5 4 Changing parameters during the transfer 52 5 5 After the transfer is complete 54 6 Mai...

Страница 3: ...of safety notices regula tory information and intended use of the SEMIPHOR TRANSPHOR UNIT series In this chapter See page Section 4 1 1 About this manual 6 1 2 Important user information 8 1 3 Regulatory information SEMIPHOR TRANSPHOR UNIT series Operating Instructions 29281777 AA 3 1 Introduction ...

Страница 4: ...the SEMIPHOR TRANSPHOR UNIT or LARGE SEMIPHOR TRANSPHOR UNIT Illustrations The images and annotations in this document are for illustrative purposes only The configuration of individual products may vary and therefore illustrations may not reflect the actual system delivered Typographical conventions In this document all the LARGE SEMIPHOR TRANSPHOR UNIT SEMIPHOR TRANSPHOR UNIT PWR LARGE SEMIPHOR ...

Страница 5: ... colon separates menu levels thus File Open refers to the Open command in the File menu Hardware items are identified in the text by bold text for example Power SEMIPHOR TRANSPHOR UNIT series Operating Instructions 29281777 AA 5 1 Introduction 1 1 About this manual ...

Страница 6: ...ill the needs of the users in academia and in life sciences industry PWR LARGE SEMIPHOR TRANSPHOR UNIT and PWR SEMIPHOR TRANSPHOR UNIT have a built in power supply LARGE SEMIPHOR TRANSPHOR UNIT and SEMIPHOR TRANSPHOR UNIT require an external power supply SEMIPHOR TRANSPHOR UNIT series shall not be used in any clinical procedures or for diagnostic purposes Prerequisites In order to operate the SEMI...

Страница 7: ...tuation which if not avoided could result in minor or moderate injury It is important not to pro ceed until all stated conditions are met and clearly understood NOTICE NOTICE indicates instructions that must be followed to avoid damage to the product or other equipment Notes and tips A note is used to indicate information that is important for trouble free and optimal use of the product Note A tip...

Страница 8: ...g information Information Requirement GE Healthcare Bio Sciences AB Name and address of manufacturer Björkgatan 30 SE 751 84 Uppsala Sweden In this section See page Section 9 1 3 1 EU directives 10 1 3 2 Eurasian Customs Union 11 1 3 3 Regulations for USA and Canada 13 1 3 4 Other regulations and standards 8 SEMIPHOR TRANSPHOR UNIT series Operating Instructions 29281777 AA 1 Introduction 1 3 Regul...

Страница 9: ... Directive Electromagnetic Compatibility EMC Directive 2014 30 EU Low Voltage Directive LVD 2014 35 EU Restriction of Hazardous Substances RoHS Directive 2011 65 EU CE marking The CE marking and the corresponding EU Declaration of Conformity is valid for the in strument when it is used according to the Operating Instructions or user manuals and used in the same state as it was delivered from GE He...

Страница 10: ...Requirement See Manufacturing information Name and address of manufacturer Telephone 46 771 400 600 Telephone number of manufacturer GE Healthcare LLC Importer and or company for obtain ing information about importer GE Healthcare Life Sciences Presnenskaya nab 10C 12th floor RU 123 317 Moscow Russian Federation Telephone 1 7 495 411 9714 Fax nr 7 495 739 6932 Email LSrus ge com 10 SEMIPHOR TRANSP...

Страница 11: ...r a Class B digital device pursuant to part 15 of the FCC Rules These limits are designed to provide rea sonable protection against harmful interference in a residential installation This equip ment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instructions may cause harmful interference to radio communications However there is no guaran...

Страница 12: ...lt the dealer or an experienced radio TV technician for help 12 SEMIPHOR TRANSPHOR UNIT series Operating Instructions 29281777 AA 1 Introduction 1 3 Regulatory information 1 3 3 Regulations for USA and Canada ...

Страница 13: ...ard requirements fulfilled by this product are summarized in the table below Description Standard Safety requirements for electrical equipment for mea surement control and laboratory use Part 1 General requirements IEC EN 61010 1 UL 61010 1 CAN CSA C22 2 No 61010 1 Electrical Equipment for Measurement Control and Laboratory Use EMC requirements Part 1 General re quirements IEC EN 61326 1 Technical...

Страница 14: ...talling operating or maintaining the product all users must read and understand the entire contents of this chapter to become aware of the hazards involved In this chapter This chapter contains the following sections See page Section 15 2 1 Safety precautions 21 2 2 Labels 23 2 3 Emergency procedures 25 2 4 Recycling information 26 2 5 Declaration of Hazardous Substances DoHS 14 SEMIPHOR TRANSPHOR...

Страница 15: ...ions in this section are grouped into the following categories General precautions Personal protection Using flammable liquids Installing and moving the instrument Power supply System operation Maintenance Always follow the instructions below to avoid injury when using the SEMIPHOR TRANSPHOR UNIT series General precautions WARNING Before installing operating or maintaining the product all users mu...

Страница 16: ...ing twisting heating or allowing them to become pinned under the equipment Using damaged power cords could result in fire or electric shock If the power supply cords are damaged contact your local GE Healthcare representative for replacements WARNING Never exceed the operating limits stated in this document and on the system label Operation of the product outside these limits can damage equipment ...

Страница 17: ...rotective clothing glasses and gloves resistant to the substances used Follow local and or national regulations for safe operation and maintenance of this product WARNING Spread of biological agents The operator must take all necessary actions to avoid spreading hazardous biological agents The facility must comply with the national code of practice for biosafety Using flammable liquids WARNING A f...

Страница 18: ...el bench with adequate space for ventilation Power supply WARNING Power cord Only use power cords with approved plugs delivered or approved by GE Healthcare System operation WARNING The safety lid must be in place before connecting the power leads to a power supply WARNING Turn all power supply controls off and disconnect the power leads before removing the safety lid 18 SEMIPHOR TRANSPHOR UNIT se...

Страница 19: ...h a certified power supply capa ble of limiting the voltage and current to specified ratings Maintenance WARNING Decontaminate before maintenance To avoid personnel being exposed to potentially hazardous substances make sure that the SEMIPHOR TRANSPHOR UNIT series is properly decontaminated and sanitized before maintenance or service WARNING Disconnect power Always disconnect power from the instru...

Страница 20: ...oning Decontaminate the equipment before decom missioning to make sure that hazardous residues are removed 20 SEMIPHOR TRANSPHOR UNIT series Operating Instructions 29281777 AA 2 Safety instructions 2 1 Safety precautions ...

Страница 21: ...t be collected separately Please contact an authorized repre sentative of the manufacturer for information concerning the de commissioning of equipment This symbol indicates that the product does not contain toxic or hazardous materials in excess of the limits established by the Chi nese standard GB T 26572 Requirements of concentration limits for certain hazardous substances in electrical and ele...

Страница 22: ...tion Na tionally Recognized Testing Laboratory NRTL Serial number of the product Serial no Year YYYY and month MM of manufacture Manufac tured Safety labels The table below describes the various symbols that may be found on the product Description Symbol text Warning Read the user documentation before using the system Do not open any covers or replace parts unless specifically stated in the user d...

Страница 23: ...y shutdown of LARGE SEMIPHORTRANSPHORUNITand SEMIPHOR TRANSPHOR UNIT In an emergency situation the shut down the external power supply of LARGE SEMIPHOR TRANSPHOR UNIT and SEMIPHOR TRANSPHOR UNIT in accordance with its emergency procedure Emergency shutdown of PWR LARGE SEMIPHOR TRANSPHOR UNIT and PWR SEMIPHOR TRANSPHOR UNIT In an emergency situation the shut down PWR LARGE SEMIPHOR TRANSPHOR UNIT...

Страница 24: ...tep Make sure all connections are in place 1 Start the power supply as described in the power supply s User Manual 2 Restart PWR LARGE SEMIPHOR TRANSPHOR UNIT and PWR SEMIPHOR TRANSPHOR UNIT after emergency shutdown or power failure To restart the run after an emergency shutdown or power failure follow these steps Action Step Make sure all connections are in place 1 Press the power button 2 24 SEM...

Страница 25: ... service the different materials must be separated and recycled according to national and local environmental regulations Disposal of electrical components Waste electrical and electronic equipment must not be disposed of as unsorted municipal waste and must be collected separately Please contact an authorized representative of the manufacturer for informationconcerningthe decommissioningof the eq...

Страница 26: ... of Pollution Control Label 该标志表明本产品不含有超过中国标准GB T26572 电子信息产品中有毒有害物 质的限量要求 中限量的有毒有害物质 报废后可以进行回收处理 不能随意丢弃 This symbol indicates that this electrical and electronic product does not contain any hazardous substances above the maximum concentration value established by the Chinese standard GB T 26572 and can be recycled after being discarded and should not be casually discarded 26 SEMIPHOR TRANSPHOR U...

Страница 27: ...量要求以下 X 表示该有害物质至少在该部件的某一均质材料中的含量超出 GB T 26572规定的限量要 求 此表所列数据为发布时所能获得的最佳信息 0 Indicates that this hazardous substance contained in all of the homogeneous materials for this part is below the limit requirement in GB T 26572 X Indicates that this hazardous substance contained in at least one of the homogeneous materials used for this part is above the limit requirement in GB T 26572 Data listed in the...

Страница 28: ...overview of the SEMIPHOR TRANSPHOR UNIT series and a brief description of its function In this chapter See page Section 29 3 1 Instrument overview 31 3 2 Detailed description 28 SEMIPHOR TRANSPHOR UNIT series Operating Instructions 29281777 AA 3 System description ...

Страница 29: ... power supply and are operated and controlled from the instrument keypad This section gives an overview of the SEMIPHOR TRANSPHOR UNIT series Illustration of the LARGE SEMIPHORTRANSPHORUNITand SEMIPHOR TRANSPHOR UNIT The illustration below shows an example of SEMIPHOR TRANSPHOR UNIT or LARGE SEMIPHOR TRANSPHOR UNIT SEMIPHOR TRANSPHOR UNIT series Operating Instructions 29281777 AA 29 3 System descr...

Страница 30: ...he table below shows the transfer surface of the instruments Use with Transfer surface Instrument Suitable for transferring standard gels including those from the SE600 Ru by and the SE400 14 16 cm PWR SEMIPHOR TRANSPHOR UNIT and SEMIPHOR TRANSPHOR UNIT Suitable for transferring gels from the Ettan DALT systems 21 26 cm PWR LARGE SEMIPHOR TRANSPHOR UNIT and LARGE SEMIPHOR TRANSPHOR UNIT 30 SEMIPHO...

Страница 31: ...and SEMIPHOR TRANSPHOR UNIT 1 2 3 4 Function Part Voltage leads 1 Top electrode 2 Lower electrode 3 Circuit breaker reset 4 LARGE SEMIPHOR TRANSPHOR UNIT and SEMIPHOR TRANSPHOR UNIT require a power supply with a minimum rating of 0 to 500 mA 0 to 30 V They have safety circuits built into the instruments that limit the voltage to 30 V and the current to 0 5 A SEMIPHOR TRANSPHOR UNIT series Operatin...

Страница 32: ... Power transformer 5 PWR LARGE SEMIPHOR TRANSPHOR UNIT and PWR SEMIPHOR TRANSPHOR UNIT have a built in power supply for transferring gels This eliminates the need for an external power supply These instruments can deliver up to 30 V and 0 5 A The instruments also monitor the transfer stack resistance and can stop a transfer if large changes indicate that the buffer system is depleting 32 SEMIPHOR ...

Страница 33: ...our minutes and the voltage V Display mode cycles LED display 1 Switch instrument ON OFF Power ON OFF but ton 2 Adjust value of the selected parameter Up down arrow 3 Start stop the electrotransfer Start stop button 4 PWR LARGE SEMIPHOR TRANSPHOR UNIT and PWR SEMIPHOR TRANSPHOR UNIT can be set to the following parameters Increment Range Unit Variable 1 mA 1 to 500 Milliampere Current 5 min 5 min t...

Страница 34: ...e system is placed on a stable level bench with adequate space for ventilation Unpacking procedure Unwrap all packages carefully Inspect all visible parts for damage or missing pieces If any damage is observed record this on the receiving documents and inform your GE Healthcare representative Make sure to keep all packing material for damage claims or to use should it become necessary to return th...

Страница 35: ... supply WARNING Turn all power supply controls off and disconnect the power leads before removing the safety lid WARNING The electric components in the transfer unit base must not become wet WARNING Do not immerse the unit in water WARNING Power cord Only use power cords with approved plugs delivered or approved by GE Healthcare SEMIPHOR TRANSPHOR UNIT series Operating Instructions 29281777 AA 35 ...

Страница 36: ...roved by GE Healthcare In this chapter See page Section 37 5 1 Assemble transfer stack 45 5 2 Electrotransfer with LARGE SEMIPHOR TRANSPHOR UNIT and SEMIPHOR TRANSPHOR UNIT 47 5 3 Electrotransfer with PWR LARGE SEMIPHOR TRANSPHOR UNIT and PWR SEMIPHOR TRANSPHOR UNIT 50 5 4 Changing parameters during the transfer 52 5 5 After the transfer is complete 36 SEMIPHOR TRANSPHOR UNIT series Operating Inst...

Страница 37: ...efer to Section 8 1 Specifications on page62 for the minimum and maximum stack thickness of SEMIPHOR TRANSPHOR UNIT series This section describes how to assemble the transfer stack Illustration of a transfer stack with a single gel 1 2 3 4 5 Function Part Blotting paper 2 to 3 sheets 1 Gel 2 Membrane 3 Blotting paper 2 to 3 sheets 4 SEMIPHOR TRANSPHOR UNIT series Operating Instructions 29281777 AA...

Страница 38: ...Cut an opening in the center of a Mylar mask Make sure that the size of the opening is roughly 2 mm smaller than the gel WARNING Be careful when cutting the masks The blades are sharp and may cause cuts 3 Place the mask in the base of the unit Make sure that the opening of the mask is in the center of the base Calculate the number of blotting paper layers Divide the volume of buffer required for t...

Страница 39: ...s with methanol 9 Soak all membrane types in transfer buffer for 2 to 5 minutes 10 Assemble a transfer stack with a single gel Follow the instructions below to assemble the transfer stack Always wear gloves when handling membranes to avoid leaving fingerprints Note Action Step One by one center each sheet on the lower electrode Refer to Illustration of a transfer stack with a single gel on page 37...

Страница 40: ...egin to transfer immediately once transfer has begun moving the gel will distort results or cause shadow bands on the blot Cover the gel with three layers of saturated blotting paper 5 Illustration of a transfer stack with multiple gels The following illustration shows a parallel transfer stack NOTICE Make sure that there is no electrical contact between the two stacks 40 SEMIPHOR TRANSPHOR UNIT s...

Страница 41: ...otting paper 2 to 3 sheets 4 Optional Mylar mask opening approximately 2 mm smaller than the gels on all sides 5 The illustration below shows a vertical transfer stack SEMIPHOR TRANSPHOR UNIT series Operating Instructions 29281777 AA 41 5 Operation 5 1 Assemble transfer stack ...

Страница 42: ...ting paper 2 to 3 sheets 1 Gel 2 Membrane 3 Blotting paper 1 sheet 4 Cellophane 5 Gel 6 Membrane 7 Blotting paper 2 to 3 sheets 8 42 SEMIPHOR TRANSPHOR UNIT series Operating Instructions 29281777 AA 5 Operation 5 1 Assemble transfer stack ...

Страница 43: ...ction Step Follow the instructions in Preparations on page 38 1 Follow the instructions in Assemble a transfer stack with a single gel on page 39 while referring to Illustration of a transfer stack with multiple gels on page 40 2 NOTICE Stack each layer with care with edges parallel As each layer is added remove all air pockets by rolling a clean pipet from the center to the edges Add a few drops ...

Страница 44: ...s by rolling a clean pipet from the center to the edges Add a few drops of buffer to trouble areas to help remove air pockets Note For best results the transfer stack should be centered on the electrode panels Continue the stack as shown in Illustration of a transfer stack with multiple gels on page 40 3 Cut a porous cellophane sheet to size that is slightly smaller than the gel Note Cellophane pe...

Страница 45: ...er using LARGE SEMIPHOR TRANSPHOR UNIT and SEMIPHOR TRANSPHOR UNIT Recommended current settings Use the graph to quickly find the maximum current setting for your gel size or calculate the gel area cm2 and multiply it by 0 8 mA Gel size cm2 Maximum current mA 14 24 14 16 8 10 8 7 500 400 300 200 100 0 0 100 200 300 400 45 65 180 270 SEMIPHOR TRANSPHOR UNIT series Operating Instructions 29281777 AA...

Страница 46: ...re that the maximum current does not exceed 0 8 mA cm2 of the gel surface or 500 mA maximum 4 If available set time for the transfer on the power supply 5 Most transfers are complete within one hour but larger proteins proteins from native gels thicker gels and transferring several layers of gels may require an additional 1 hour of transfer time The optimum transfer time for each protein and gel s...

Страница 47: ...er using PWR LARGE SEMIPHOR TRANSPHOR UNIT and PWR SEMIPHOR TRANSPHOR UNIT Recommended current settings Use the graph to quickly find the maximum current setting for your gel size or calculate the gel area cm2 and multiply it by 0 8 mA Gel size cm2 Maximum current mA 14 24 14 16 8 10 8 7 500 400 300 200 100 0 0 100 200 300 400 45 65 180 270 SEMIPHOR TRANSPHOR UNIT series Operating Instructions 292...

Страница 48: ...WN arrows and set the current Note The transfer cannot be set to run at constant voltage Toggle with the DISPLAY MODE button to the time hour minutes 3 Change the value with the UP and DOWN arrows and set the time Note While setting the time there is an extra setting labelled on above the 4 00 upper limit This will disable the timer allowing the transfer to run continuously until manually turned o...

Страница 49: ...sult A red LED will appear in the lower right corner of the display when voltage is applied to the transfer stack as shown in the illustration below SEMIPHOR TRANSPHOR UNIT series Operating Instructions 29281777 AA 49 5 Operation 5 3 Electrotransfer with PWR LARGE SEMIPHOR TRANSPHOR UNIT and PWR SEMIPHOR TRANSPHOR UNIT ...

Страница 50: ... the electrotransfer run Follow the instructions below to change values during a run Action Step Select mA or time using the DISPLAY MODE button 1 Change the value with the UP and DOWN buttons While the value is being changed the red LED will blink 2 Result About 10 seconds after the values have been changed the LED will stop flashing and show the real time run parameters 50 SEMIPHOR TRANSPHOR UNI...

Страница 51: ...d lead to further problems like burning of the transfer stack Follow the instructions below to add buffer when the buffer is depleted during the elec trotransfer Action Step Check if the display shows the error message dry as shown in the illustration below 1 Note The instrument has stopped the transfer Remove the cover slowly because the stack may adhere to it 2 Add buffer to the transfer stack 3...

Страница 52: ...ll blink and cycle between the values for the current time and voltage at the time the transfer was stopped These can be recorded into a notebook if desired Pushing any button will clear the display Remove gel from transfer stack Follow the instructions below to take the gel and the membrane out of the transfer stack Action Step Remove the cover slowly because the stack may adhere to it 1 Remove a...

Страница 53: ...col or allow the membrane to air dry prior to storage Note Re wet dried membranes in the appropriate wetting buffer prior to processing 7 Remove the remaining blot papers and dispose of them 8 Rinse the unit according to the instructions in Chapter 6 Maintenance on page 54 9 SEMIPHOR TRANSPHOR UNIT series Operating Instructions 29281777 AA 53 5 Operation 5 5 After the transfer is complete ...

Страница 54: ...ed and sanitized before maintenance or service WARNING Use only approved parts Only spare parts and accessories that are approved or supplied by GE Healthcare may be used for maintaining or servicing the product WARNING Disconnect power Always disconnect power from the instrument before performing any maintenance task WARNING Do not immerse the unit in water CAUTION Never autoclave any component o...

Страница 55: ...unit with a cleaning agent Never use abrasive cleansers Cleaning before planned maintenance service To ensure the protection and safety of service personnel all equipment and work areas must be clean and free of any hazardous contaminants before a Service Engineer starts maintenance work Please complete the checklist in the On Site Service Health and Safety Declaration Form or the Health and Safet...

Страница 56: ...ver exceed the operating limits stated in this document and on the system label Operation of the product outside these limits can damage equipment and cause personal injury or death WARNING Do not immerse the unit in water CAUTION Never autoclave any component of the electrophoresis unit or casting kit Incomplete transfer Solution Problem Remove trapped air pockets between the gel and membrane dur...

Страница 57: ...M sodium dodecyl sulfate SDS 0 1 in the transfer buffer Add several more sheets of buffer saturated blotting paper to each side of the gel sandwich so that more buffer is present during the transfer If using a non nitrocellulose membrane avoid in cluding methanol in the transfer buffer or reduce the amount to the minimum possible Use reagent grade chemicals Increase the net charge on the protein b...

Страница 58: ... to the cold room during equilibration Diffuse band patterns If transfer buffer contains methanol 10 equilibrate the gel in transfer buffer for 30 minutes to allow it to shrink before assembling the stack Note Because methanol causes the gel to shrink slightly large molecules may migrate more slowly Make sure that the gel is held firmly against the membrane and that it does not shift once contact ...

Страница 59: ...0 methanol to the transfer buffer to enhance binding to nitrocellulose Wear gloves when handling membranes Membrane parameters Store membranes at ambient temperature out of direct sunlight to keep the membranes activated Check shelf life of nitrocellulose membrane re place if necessary Use a membrane with a smaller pore size 0 10 to 0 20 µm if proteins pass through the membrane or use a different ...

Страница 60: ... stack is not making contact with the up per electrode Add blot papers soaked in buffer No output current Instrument has detected large changes in transfer stack resistance dry error message Add more buffer soaked blot papers in future transfers Transfer stack is being short circuited No output voltage 60 SEMIPHOR TRANSPHOR UNIT series Operating Instructions 29281777 AA 7 Troubleshooting ...

Страница 61: ...ries The chapter also includes ordering information and Health and Safety Declaration form for service In this chapter See page Section 62 8 1 Specifications 64 8 2 Ordering information 65 8 3 Health and Safety Declaration Forms SEMIPHOR TRANSPHOR UNIT series Operating Instructions 29281777 AA 61 8 Reference information ...

Страница 62: ...DC 0 5 0 5 Current A 3 2 3 2 Minimum transfer stack thick ness mm 12 0 12 0 Maximum transfer stack thick ness mm Indoor use 4 C to 40 C Environmental operating condi tions Humidity up to 80 relative humidity Altitude up to 2000 m II Installation category 2 Pollution degree 38 46 9 cm Dimensions W H D 3 7 kg Weight 1 Maximum setting for the customer s power supply 62 SEMIPHOR TRANSPHOR UNIT series ...

Страница 63: ...100 to 240 Voltage VAC 50 60 50 60 Frequency Hz 0 4 0 4 Current A Output 30 30 Voltage VDC 0 5 0 5 Current A 2 8 2 8 Minimum transfer stack thick ness mm 12 0 12 0 Maximum transfer stack thick ness mm Indoor use 4 C to 40 C Environmental operating condi tions Humidity up to 80 relative humidity Altitude up to 2000 m II Installation category 2 Pollution degree 38 46 9 cm Dimensions W H D 3 7 kg Wei...

Страница 64: ...nformation For product codes and information about how to order please see www gelifesciences com 64 SEMIPHOR TRANSPHOR UNIT series Operating Instructions 29281777 AA 8 Reference information 8 2 Ordering information ...

Страница 65: ...hersuitablesurvey Adequate space andclearance is providedto allowsafe access forinstrumentservice repairor installation Insome cases this mayrequirecustomerto move equipmentfrom normaloperatinglocation prior to GE arrival Consumables suchas columns orgels have beenremovedor isolatedfromthe instrument andfrom any areathat may impede access tothe instrument All buffer waste vessels are labeled Exces...

Страница 66: ...rdous biological substances pleasespecify OtherHazardous Chemicals please specify Equipment must be decontaminatedpriortoservice return Pleaseprovide atelephone number whereGE cancontact youfor additionalinformationconcerningthe system equipment Telephone No Liquid and orgas in equipment is Water Ethanol None empty Argon Helium Nitrogen Liquid Nitrogen Other please specify Equipment type ProductNo...

Страница 67: ...NIT 24 shutdown 23 Emergency procedures 23 End of run 52 F FCC compliance 11 I Important user information 6 Incomplete transfer 56 Intended use 6 L Labels 21 Safety labels 22 LARGE SEMIPHOR TRANSPHOR UNIT and SEMIPHOR TRANSPHOR UNIT Emergency shutdown 23 instrumentmodules descrip tions 31 run 46 M Manufacturing information 8 Membrane inefficient binding 59 Multiple transfer stack parallel 40 verti...

Страница 68: ...ions introduction 15 Select current LARGE SEMIPHOR TRANSPHOR UNIT and SEMIPHOR TRANSPHOR UNIT 45 47 Service Cleaning before 55 Single transfer stack 37 Standards 13 System description 29 30 System label 21 T Transfer stack assemble 37 maximum thickness 62 63 minimum thickness 62 63 remove membrane 52 Transfer surface 30 Troubleshooting 56 diffuse bands 58 incomplete transfer 56 inefficient binding...

Страница 69: ...Healthcare repre sentative for the most current information GE Healthcare Bio Sciences AB Björkgatan 30 751 84 Uppsala Sweden GE Healthcare Europe GmbH Munzinger Strasse 5 D 79111 Freiburg Germany GE Healthcare Bio Sciences Corp 100 Results Way Marlborough MA 01752 USA HyClone Laboratories Inc 925 W 1800 S Logan UT 84321 USA GE Healthcare Japan Corporation Sanken Bldg 3 25 1 Hyakunincho Shinjuku k...

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