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GE Frac-920, Руководство по эксплуатации

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Flow path

Connect inlet and outlet tubing to the system. See

Fraction Collector Frac-920 and

Frac-950 User Manuals

.

Electrical power

Connect the power cord to a grounded power outlet specified in

Section 3.1 Site require-

ments, on page 25

.

3.6

Spare parts and accessories

For correct up to date information on spare parts and accessories visit:
www.gelifesciences.com/AKTA

Frac-920 and Frac-950 Operating Instructions 28-9622-09 AC

27

3 Installation

3.5 Connections

Содержание Frac-920

Страница 1: ...Fraction Collector Frac 920 and Frac 950 Operating Instructions Original instructions ...

Страница 2: ...ite requirements 25 3 2 Transport 26 3 3 Unpacking 26 3 4 Assembly 26 3 5 Connections 27 3 6 Spare parts and accessories 28 4 Operation 28 4 1 Fraction Collector Frac 920 stand alone 37 4 2 Fraction Collector Frac 920 connected to an ÄKTA instrument 39 4 3 Fraction Collector Frac 950 47 5 Maintenance 47 5 1 General 47 5 2 Cleaning 48 5 3 Changing capillaries 48 5 4 Changing waste tubing 49 6 Troub...

Страница 3: ...em works if a computer is used The user must understand the concepts of liquid chromatography The user must read and understand the Safety Instructions in this manual FractionCollectorFrac 920and Frac 950and software should be installed configured and calibrated according to these Operating Instructions About this chapter This chapter contains important user information a description of the intend...

Страница 4: ...ated fraction collectors for use in ÄKTA chromatography systems Fraction Collector Frac 920 can also be used as a stand alone unit Fraction Collector Frac 920 and Frac 950 are intended for research use only and shall not be used in any clinical procedures or for diagnostic purposes Safety notices This user documentation contains WARNINGS CAUTIONS and NOTICES concerning the safe use of the product ...

Страница 5: ...mal use of the product Note A tip contains useful information that can improve or optimize your procedures Tip Typographical conventions Software items are identified in the text by bold italic text A colon separates menu levels thus File Open refers to the Open command in the File menu Hardware items are identified in the text by bold text e g Power switch 1 2 Regulatory information In this secti...

Страница 6: ...ng harmonized standards Title Directive Machinery Directive MD 2006 42 EC Electromagnetic Compatibility EMC Directive 2004 108 EC Low Voltage Directive LVD 2006 95 EC CE marking The CE marking and the corresponding EU Declaration of Conformity is valid for the in strument when it is used as a stand alone unit or connected to other products recommended or described in the user documentation and use...

Страница 7: ...id the user s authority to operate the equipment Note This equipment has been tested and found to comply with the limits for a Class B digital device pursuant to part 15 of the FCC Rules These limits are designed to provide rea sonable protection against harmful interference in a residential installation This equip ment generates uses and can radiate radio frequency energy and if not installed and...

Страница 8: ...e 2011 65 EU Waste Electrical and Electronic Equipment WEEE Directive 2012 19 EU Administration on the Control of Pollution Caused by Electronic Information Products China Restriction of Hazardous Sub stances RoHS ACPEIP Registration Evaluation Authorization and restriction of CHemicals REACH Regulation EC No 1907 2006 1 3 Instrument Fraction Collector Frac 920 and Frac 950 are automated fraction ...

Страница 9: ...ion Collector Frac 920 Description Part Delivery arm 1 Lock knob 2 Control buttons 3 Control buttons 4 Collection tubes 5 Tube rack 6 Programming buttons 7 Menu display 8 Frac 920 and Frac 950 Operating Instructions 28 9622 09 AC 9 1 Introduction 1 3 Instrument 1 3 1 Fraction Collector Frac 920 ...

Страница 10: ...ions Description Part Drop sensor 1 Valve 2 Auxillary equipment 3 Remote digital I O 4 UniNet 1 5 Power inlet 6 10 Frac 920 and Frac 950 Operating Instructions 28 9622 09 AC 1 Introduction 1 3 Instrument 1 3 1 Fraction Collector Frac 920 ...

Страница 11: ...andar d m ode 1 2 3 4 5 1 2 3 4 5 1 2 3 4 5 Table 1 2 The main part of Fraction Collector Frac 950 Description Part Delivery arm 1 Safety bar 2 Collection tubes 3 Tube rack 4 Indicators 5 Frac 920 and Frac 950 Operating Instructions 28 9622 09 AC 11 1 Introduction 1 3 Instrument 1 3 2 Fraction Collector Frac 950 ...

Страница 12: ...nder Microsoft Windows operating system For more information about UNICORN control system see the UNICORN user manuals supplied 1 5 User documentation In addition to these Operating Instructions the documentation package supplied with Fraction Collector Frac 920 and Frac 950 also includes product documentation binders containing detailed specifications and traceability documents Themostimportantdo...

Страница 13: ...ty for Fraction Collector Frac 920 and Frac 950 Software documentation Together with each system the following software documentation is supplied providing additional information that applies to Fraction Collector Frac 920 and Frac 950 indepen dent of the specific configuration Purpose Contents Document The manuals contain detailed instructions on howto administerUNICORN workwithmethods perform ru...

Страница 14: ...l Follow the instructions provided to avoid personal injury or damage to the equipment The safety precautions in this section are grouped into the following categories General precautions Using flammable liquids Personal protection Installing and moving the instrument System operation Maintenance General precautions Always follow these General precautions to avoid injury when using Fraction Collec...

Страница 15: ...e for example damage to the power cord or its plug damage caused by dropping the equipment damage caused by splashing liquid onto it WARNING Operation and user maintenance of Fraction Collector Frac 920 and Frac 950 should be performed by properly trained personnel only WARNING Do not use the Fraction Collector Frac 950 if the safety bar is bro ken CAUTION Waste tubes and containers must be secure...

Страница 16: ...rotection WARNING Always use appropriate personal protective equipment during operation and maintenance of Fraction Collector Frac 920 and Frac 950 WARNING When using hazardous chemical and biological agents take all suitable protective measures such as wearing protective glasses and gloves resistant to the substances used Follow local and or nationalregulationsfor safe operationand maintenanceof ...

Страница 17: ...ing on the equipment before con necting the power cord WARNING Fraction Collector Frac 920 and Frac 950 must always be connect ed to a grounded power outlet WARNING Power cord Only use power cords with approved plugs delivered or approved by GE Healthcare WARNING Access to power switch and power cord with plug Do not block access to the power switch and power cord The power switch must always be e...

Страница 18: ...by a neutral buffer and finally distilled water before service and maintenance CAUTION Pinch hazard Do not have any part of your body within the unit base area when Fraction Collector Frac 950 is switched on An automatic calibration process starts when Fraction Collector Frac 950 is connected to UNICORN During calibration the dis penser arm moves rapidly Several beeps are heard before the calibrat...

Страница 19: ...this section This section describes the instrument labels and labels concerning hazardous substances that are attached to the Fraction Collector Frac 920 and Frac 950 instrument For infor mation about marking of the computer equipment refer to the manufacturer s instruc tions Labels on the instrument The illustration below shows an example of the identification label that is attached to Fraction C...

Страница 20: ...y Recognized Testing Laboratory NRTL NRTL means an organization which is recognized by the US Occupational Safety and Health Administration OSHA as meeting the legal requirements of Title 29 of the Code of Federal Regulations 29 CFR Part 1910 7 Labels concerning hazardous substances Meaning Label This symbol indicates that the waste of electrical and electronic equip ment must not be disposed as u...

Страница 21: ...nt of power failure Emergency shutdown In an emergency situation do as follows to stop the run Action Step Press the Main power switch to the 0 position The run is interrupted imme diately 1 Power failure The result of a power failure depends on which unit that is affected will result in Power failure to The run is interrupted immediately in an undefined state Fraction Collector Frac 920 and Frac ...

Страница 22: ...al environmental regulations Recycling of hazardous substances Fraction Collector Frac 920 and Frac 950 contains hazardous substances Detailed in formation is available from your GE Healthcare representative Disposal of electrical components Waste of electrical and electronic equipment must not be disposed as unsorted municipal waste and must be collected separately Please contact an authorized re...

Страница 23: ...d EFUP which indicates the period during which the toxic or hazardous substances or elements contained in electronic information products will not leak or mutate under normal operating con ditions so that the use of such electronic information products will not result in any severe environmental pollution any bodily injury or damage to any assets The unit of the period is Year In order to maintain...

Страница 24: ...的限量要求 此表所列数据为发布时所能获得的最佳信息 0 Indicates that this toxic or hazardous substance contained in all of the homogeneous ma terials for this part is below the limit requirement in SJ T11363 2006 X Indicates that this toxic or hazardous substance contained in at least one of the homoge neous materials used for this part is above the limit requirement in SJ T11363 2006 Data listed in the table represents be...

Страница 25: ...2000 m Altitude 100 240 V AC 10 50 60 Hz Electrical power Overvoltage category II Transient overvoltages 4 C to 40 C Ambient temperature Stable laboratory bench Placement 20 to 95 non condensing Humidity 2 Pollution degree 3 2 Transport The equipment can be transported on a trolley capable of supporting at least 20 kg NOTICE Always lift Fraction Collector Frac 920 and Frac 950 by the base unit nev...

Страница 26: ...thcare representative Unpack the system Remove straps and packing material Then set the equipment upright before starting installation 3 4 Assembly The following parts must be added to the Fraction Collector Frac 920 and Frac 950 in strument before it can be used Tube rack Collection tubes Tubing Waste tube 3 5 Connections Communication Make sure that UNICORN control software is installed on the c...

Страница 27: ...r Connect the power cord to a grounded power outlet specified in Section 3 1 Site require ments on page 25 3 6 Spare parts and accessories For correct up to date information on spare parts and accessories visit www gelifesciences com AKTA Frac 920 and Frac 950 Operating Instructions 28 9622 09 AC 27 3 Installation 3 5 Connections ...

Страница 28: ...play for example system name and software version number and a beep is heard If an error is detected during the self test an error message is shown 3 The self test takes about five seconds When the start up is completed the display shows the Run Method menu Menu display See Fraction Collector Frac 920 for the layout of the Frac 920 front panel Press or to select a specific menu Press OK to enter a...

Страница 29: ... methods To reach the Program Method menu press See Pro gramming a method on page 31 Setup and Check is used for setup and checking internal system parameters such as Fraction Base and Delay UV to Frac For more information refer to Fraction col lector Frac 920 User Manual To reach the Setup and Check menu press again Changing a parameter value Description No Parameter 1 Frac 920 and Frac 950 Opera...

Страница 30: ...e set value in the Set Delay UV to Frac menu The control button feed tube is disabled if connected to CAN bus If the pump is off the tube feed is done without a delay Press end to interrupt method operationbefore the method is completed Press pump on off to start stop the pump When no method is running the pump can be used for e g buffer exchange or equilibration Press pause cont to pause all oper...

Страница 31: ...thod base should be checked for more information refer to Fraction Collector Frac 920 User Manual Note Creating a new method To create a new method 1 From the Run Method menu press to reach the Program Method menu 2 Press OK 3 The number of free methods is displayed 4 Select a free method and press OK Editing a method To edit a method Frac 920 and Frac 950 Operating Instructions 28 9622 09 AC 31 4...

Страница 32: ...Select a method number to clear Press OK 2 Select clear 3 To confirm the deletion select yes Press OK Setting fraction parameters When a new method is created or a current is edited the first displayed menu will be Set Fraction Size 1 Press OK and select a fraction size using the arrow buttons 2 Press OK 3 To set fraction start press Press OK 4 Set a start value 32 Frac 920 and Frac 950 Operating ...

Страница 33: ...ct no Press OK The flow rate should be 5 ml min to make drop synchronization possible Note Setting peak detection The peak detecting function can be set to Off Level or Slope To skip peak detection press 1 To activate a peak detecting function Press OK 2 Select Peak Detect Level or Slope Press OK If Level has been selected set an absorbance level FSD when to start collecting fractions If Slope has...

Страница 34: ...he peak detection parameters refer to Fraction Collector Frac 920 User Manual Saving the method 1 Decide to save the method select yes or no 2 Press OK 34 Frac 920 and Frac 950 Operating Instructions 28 9622 09 AC 4 Operation 4 1 Fraction Collector Frac 920 stand alone ...

Страница 35: ... Description No Description No Run menu 2 2 Run menu 1 1 1 Use the Esc and arrow buttons to reach the Run Method menu 2 Press OK 3 Select which method to run Frac 920 and Frac 950 Operating Instructions 28 9622 09 AC 35 4 Operation 4 1 Fraction Collector Frac 920 stand alone ...

Страница 36: ...ription No Method no 1 Method status 2 Method run length 3 Tube no 4 Actual tube volume fraction or peak fraction size Tube volume is counted from 0 to set value 5 Run menu 2 Description No Flow ml min if a valid pump is connected 1 36 Frac 920 and Frac 950 Operating Instructions 28 9622 09 AC 4 Operation 4 1 Fraction Collector Frac 920 stand alone ...

Страница 37: ...e achieved automatically from a preprepared method or manually via the functions available in UNICORN Fraction Collector Frac 920 are designated Frac 900 in UNICORN Note When Fraction Collector Frac 920 is controlled from UNICORN all menu push buttons are disabled Note The manual instructions in UNICORN allow the following operations Collecting a fixed volume in each tube Collecting each peak in a...

Страница 38: ...d the message Setup and check is displayed Flow during tube change The sample flow during tube change can be handled in two different ways The selections can be found under menu System Control System Settings Specials 1 To change the settings select the instruction FracParameters 2 For the parameter TubeChange select one of the following options Tube No synchronization of collection DropSync Tube ...

Страница 39: ... not have any part of your body within the unit base area when Fraction Collector Frac 950 is switched on An automatic calibration process starts when Fraction Collector Frac 950 is connected to UNICORN During calibration the dis penser arm moves rapidly Several beeps are heard before the calibration procedure starts CAUTION Be sure to fold down the safety bar whenever the rack holder is operated ...

Страница 40: ...rsion 3 21 or higher for standard mode or version 4 0 or higher for prep mode For ÄKTAmicro UNICORN version 5 0 or higher is recommended Control of Fraction Collector Frac 950 can be achieved automatically from a method or manually via the functions available in UNICORN Using Fraction Collector Frac 950 in a method is described in the ÄKTA design Optional Configuration User Manual The following fu...

Страница 41: ...or Frac 950 This value must be changed when the ÄKTA standard configuration system is changed to an op tional configuration Collecting fixed fractions Details about collecting fixed fraction volumes using UNICORN in a method are described in the ÄKTA design Optional Configuration User Manual Collecting peak fractions Details about collecting peaks only using Fraction Collector Frac 950 in a method...

Страница 42: ... defined in the start protocol to have the last tube can be ignored with the instruction Ignore_LastTube When the last tube is reached and there are more fractions to collect an alarm is gen erated and the system is paused You can then fill up with new tubes and use the instruc tion Ignore_LastTube 1 Select menu System Control Manual Frac in UNICORN 2 Select the instruction Ignore_LastTube in the ...

Страница 43: ... button AccumulatorWash The accumulator used to eliminate spillage at tube change can be manually washed with the instruction AccumulatorWash 1 Start a flow of 10 ml min manually using the system pump 2 If the fraction collector is connected to any other port than port 1 in the Outlet valve you must manually switch Outlet Valve to the other port Select menu System Con trol Manual Flowpath in UNICO...

Страница 44: ...CORN 1 In UNICORN select menu System Control System Settings 2 Click the Specials radio button and select instruction FracParameters The DelayVol instruction becomes highlighted 3 To change the setting click on the up and down arrows for the DelayVol parameter or type a new value directly in the parameter window 4 The appropriate value for your ÄKTA system is found in the ÄKTA design Optional Conf...

Страница 45: ... 75 mm tubing between the DropSync and the accumulator Accumulator During tube change the flow is diverted to the accumulator which stores the liquid When the new tube is in position the liquid is pressed out rapidly for col lection 4 Flow rate limit recommendations using DropSync without spillage are given in the table below For higher flow rates the accumulator is recommended for spillage free f...

Страница 46: ...When running a method the rack and tube parameters for the rack to be used must be set This is described in detail in the ÄKTA design Optional Configuration User Manual 46 Frac 920 and Frac 950 Operating Instructions 28 9622 09 AC 4 Operation 4 3 Fraction Collector Frac 950 ...

Страница 47: ...n the capillaries and the accumulator in Fraction Collector Frac 950 with distilled water The instrument should be wiped regularly with a damp cloth Remember to wipe the DropSync unit photocell as well Allow the instrument to dry completely before use Cleaning before planned maintenance service To ensure the protection and safety of service personnel all equipment and work areas must be clean and ...

Страница 48: ...es when they show signs of leakage or wear sharp bending for example 5 4 Changing waste tubing Change the waste tubing when it shows signs of wear 48 Frac 920 and Frac 950 Operating Instructions 28 9622 09 AC 5 Maintenance 5 3 Changing capillaries ...

Страница 49: ...and or replaced Fraction Collector Frac 950 only CAUTION Only spare parts that are approved or supplied by GE Healthcare may be used for maintaining or servicing the unit 6 1 Faults and actions Action Fault Start a flow and start fractionation Select FeedTube from the menu SystemControl Man ual Flowpath If the motor does not start and an error appears contact GE Healthcare Check the delay volume A...

Страница 50: ...func tioning Check that the safety bar is folded up Nofractionsarecollect ed Check that the DropSync unit is close enough over the tubes Check that the rack is correctly fitted to the rack holder Check that the capillary end is cut cleanly and straight Check that the correct rack type is selected Liquid misses the tubes 50 Frac 920 and Frac 950 Operating Instructions 28 9622 09 AC 6 Troubleshootin...

Страница 51: ...0 Hz Supply voltage 300 VA Frac 950 Power consumption 20 VA Frac 920 480 380 550 mm Frac 950 Dimensions H W D 250 320 400 mm Frac 920 16 5 kg Frac 950 Weight 4 5 kg Frac 920 4 to 40 C Ambient temperature 20 to 95 non condensing Relative humidity tolerance 84 to 106 kPa 840 to 1060 mbar Atmospheric pressure 73 dB A Acoustic noise level Frac 920 and Frac 950 Operating Instructions 28 9622 09 AC 51 7...

Страница 52: ...en removed fr above has bee onnel and our c ce Engineer st e Service Engi may lead to de swer Yes or ers in box belo ous substance scanner surfac ument is clean ed to allow saf re customer to have been rem e instrument from the area Seria en cleaned to Comp Date GE and GE GE Healthc NJ 08855 1 2010 14 On Site Safety customers all arts a repair T neer upon arr lays in servicin No ow es ces or other...

Страница 53: ...e specify service retu e system equ Wate Ethan None Argo Liqui lease d above has b ervice numbe er service Healt for Pr and or Service Ticket nel our custom aminants befo with your retu cing or return return to GE m d additional cl n in contact wi ces please sp y rn Please pro uipment er nol e empty n Helium Nit d Nitrogen Seria been cleaned t Comp instit Date er GE and GE GE Healthc NJ 08855 1 20...

Страница 54: ...7 3 Ordering information For ordering information visit www gelifesciences com AKTA 54 Frac 920 and Frac 950 Operating Instructions 28 9622 09 AC 7 Reference information 7 3 Ordering information ...

Страница 55: ...2009 All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them A copy of these terms and conditions is available on request Contact your local GE Healthcare repre sentative for the most current information GE Healthcare Europe GmbH Munzinger Strasse 5 D 79111 Freiburg Germany GE Healthcare UK Limited Amersham Place Little Ch...

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