ÄKTA start System Cue Card, 29-0240-42 AC
8
Evaluating a run
Save Result as BMP image
The system generates a BMP file of the result which can be
viewed under any OS that has an image viewer supporting
BMP. The chromatogram is plotted as UV abs (mAU) vs
Volume (ml), along with Fractionation marks.
Note: BMP file is generated only for the last 4 hours of run.
Evaluating a run using UNICORN start
Evaluation
module of UNICORN start is used to manage
and evaluate the results from chromatography runs. For
instructions to evaluate results that are generated using
ÄKTA start, refer to the
UNICORN start 1.0 User Manual
.
Save Result to USB memory stick
Import Result to UNICORN start
The result file stored on the USB stick can be imported to
UNICORN start for analysis, printing and generating a report.
Quick start methods
Quick start
contains “ready to run” methods like Affinity,
Ion exchange, Gel filtration and Desalting. The run
parameters are pre-set in the method. Details of
sample
volume
need to be provided before starting the run.
If required, the run parameters can be changed (
Edit
run
in Run view
screen) when the run is in progress.
Use appropriate columns as indicated in the template
names. E.g., use 1 ml HiTrap™ column in case of
selecting the Quick start
AC/IEX step 1 ml HiTrap
, or 5 ml
column when selecting
AC/IEX step 5 ml HiTrap
.
Quick start
workflow
•
•
Connect USB stick to
ÄKTA start
Select
chromatography
method (for method run)
Manual run / Method run
In the
Run parameters
or
Manual run
screens, tick the
checkbox
Save Result to USB
Import and view
Result file
on
UNICORN start or BMP image
on any other OS.
UNICORN start
File : Import
Import
Result
from USB stick:
• Locate USB drive
• Locate and select the result
file. Select multiple files using
Shift
or
Ctrl
keys.
Open
Select destination folder
for result
Evaluation
Import
Pre defined
UNICORN start
System control
Method run
Quick start
AC / IEX / DS / GF
Note: The result files will be saved in the GE folder which
is automatically created by the instrument once the USB
memory stick is plugged in. Only 10 results can be stored
in the GE folder. To save further results, transfer the result
files to another folder, PC or rename the GE folder.
Method run
Select
the desired
technique
Quick start
Set
Sample volume
Tick the checkbox
Save Result to USB
Edit run
to alter
run parameters
ÄKTA start
End
Run
Exit
