ÄKTA start System Cue Card, 29-0240-42 AC
6
Column preparation
The
Column preparation
method is used for:
Preparing a new column
Equilibrating the column.
Note: Equilibrate columns before starting a new run.
Instruction
Immerse the buffer inlet tubing into the buffer used in
the chromatographic run.
Connect the column in the flow path.
•
•
1.
2.
Operation from the Instrument Display
Starting a run
Chromatography runs can be performed either by running
predefined methods, or by using manual operations.
Perform a method run
The following method types are available with ÄKTA start
to perform chromatography runs:
Quick start
Templates
User defined
Follow the workflows below to run predefined methods.
Operation from the Instrument Display
•
•
•
End
Run
Exit
Edit run
Method run
Prepare system
Column preparation
Set
Run parameters
to
suit your requirement
ÄKTA start
Pre defined
System preparation
Column preparation
UNICORN start
System control
Method run
End
Run
Exit
Edit run
Method run
Set
Run parameters
to
suit your requirement
ÄKTA start
Select method
• Quick start
• Templates
• User defined
Select
Pre defined
Select
method
• Quick start
• Templates
• User defined methods
Ok/Start
UNICORN start
System control
Method run
Operation from UNICORN start
Operation from UNICORN start
Tighten all connections and pump DM water through
the system to check for leaks.
Tighten any leaking connector.
Removal from cold room
Switch off the instrument and disconnect power cable.
Loosen all connections to prevent them sticking when
the system returns to room temperature.
Allow the instrument to stabilize at room temperature.
Tighten all connections and pump DM water through
system to check for leaks.
3.
4.
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2.
3.
4.
