ÄKTA start System Cue Card, 29-0240-42 AC
10
El
ut
io
n
Pu
m
p
w
as
h
Eq
ui
lib
ra
tio
n
100
50
3 CV
1.2 CV
Sa
m
pl
e
%B
Fractionation/collection
IEX Step 1 ml/5 ml HiTrap - Ion Exchange Chromatography
The bound proteins are eluted in a single step using single
elution buffer.
Re
-e
qu
ili
br
at
io
n
W
as
h
un
bo
un
d
El
ut
io
n
Pu
m
p
w
as
h
Eq
ui
lib
ra
tio
n
100
50
5 CV
5 CV
5 CV
5 CV
Sa
m
pl
e
%B
Fractionation/
collection
IEX Gradient 1 ml/5 ml HiTrap - Ion Exchange
Chromatography
The bound proteins are eluted using two buffers with linear
increase in the concentration of buffer B over a specified
time, followed by a step with 100% B concentration.
Re
-e
qu
ili
br
at
io
n
W
as
h
un
bo
un
d
El
ut
io
n
Pu
m
p
w
as
h
Eq
ui
lib
ra
tio
n
100
50
5 CV
5 CV
5 CV
5 CV
Sa
m
pl
e
%B
Fractionation/
collection
20 CV
Gr
ad
ie
nt
0
to
1
00
%
B
GF 16/60 HiPrep - Gel Filtration Chromatography
The proteins are eluted in a single step using single
elution buffer.
The column needs to be pre-equilibrated before the
start of the run.
•
•
El
ut
io
n
Pu
m
p
w
as
h
Eq
ui
lib
ra
tio
n
100
50
0.2 CV
1.2 CV
Sa
m
pl
e
%B
Fractionation/collection
Flow charts
The active flow path is depicted in Green color.
Flow path details for flow of 100% buffer A
through Outlet valve to waste
A.
Flow path details for flow of 100% buffer B
through Outlet valve to waste
B.
Flow path details during gradient
operation at specified B concentration
C.
Flow path details, Outlet valve collection
Note: Fraction collector is disabled.
D.
