Description
Button
Advance the fraction collector one position.
feed tube
Basic flow path
IN
I N
P
M
UV
C
pH
F
W
V2
V1
B
SW
Figure 1.3: Basic flow path.
Description
Part
Stage
Pump P pumps buffer from a buffer container connected to the
buffer valve V1.
P, V1
1
To form a gradient the switch valve (SW) can be used to pull liquid
from buffer container (B).
SW, B
2
The mixer (M) mixes the buffers.
M
3
Sample is applied from the sample loop connected to injection
valve (V2) that has been previously filled manually using a syringe.
V2
4
From the injection valve, the flow is directed to the column, and
then to the UV, Conductivity, and optional pH monitor.
UV, C, pH
5
From the monitors, the flow is directed to the Fraction collector
F or the Waste W.
F, W
6
12
ÄKTAprime plus Operating Instructions 28-9597-89 AC
1 Introduction
1.3 Instrument
