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FLUIGENT 

Siège social : Biopark 

 1 mail du Professeur Georges Mathé 

 94800 Villejuif 

 France 

Tel : +331 77 01 82 68  

 Fax : +331 77 01 82 70 

www.fluigent.com 

 

SA à Directoire et Conseil de Surveillance au capital de 141 284.95 

€ 

- Siret : 487 636 409 00038 

 N°TVA UE/EU VAT Number : FR 53 487 636 409 

 

6.

 

Start working with the Flow-Rate Platform  

6.1

 

Quick start procedure 

Here is a quick setup guide to remind you the main steps to get your Flow-Rate Platform up and running. 

1.

 

First, you may want to integrate the different FLOW UNIT to your microfluidic system, with the right 
fittings. See §4.2 how to do it. 

2.

 

Then, connect the FLOW UNIT models to the FLOWBOARD. See §5.2 how to do it. 

3.

 

Then connect the FLOWBOARD and the computer with the USB cable. See §5.2 how to do it. 

4.

 

To finish start the software  (FRP SFP or MAESFLO) installed on your computer (user manual) 

You can now use your Flow-Rate Platform for your application. 

   DO NOT FORGET TO CLEAN AND RINSE YOUR FLOW UNIT AFTER USE

 (See §7 how to do it.) 

6.2

 

Use at high temperature and high flow-rate 

 

The Flow Units can be used in a large range of temperature, but some elements need to be taken into account: 
 

-

 

The  Flow  Units  include  temperature  compensation  between  10°C  and  50°C.  However,  as  the 
temperature  deviates  from  20°C,  the  absolute  accuracy  may  acquire  an  additional  error  of 
typically 0.1% of the measured flow rate per °C.  
For  example  the  L  Flow  Unit  model  at  50°C  has  a  specified  error  of  5%  +  30*0.1%  =  8%  of  the 
measured value. 

-

 

Between  50°C  and  80°C  the  Flow  Unit  will  still  be  operational  and  the  repeatability  will  still  be 
excellent. However, we give no more guarantee for the absolute accuracy of the calibration. 
 

In  order  to  get  a  correct  reading  from  the  sensor,  it  is  crucial  to  have  the  liquid  temperature  and  ambient 
temperature the same (within ± 3°C). At low flow rates this 

o ’t  e a 

problem, because the liquid adapts to 

the ambient temperature very quickly. At higher flow rates (for L and XL Flow Unit models) this is important.  

6.3

 

Use a XL Flow Unit 

The  o i atio  of differe t para eters  a  lead to u a ted  eha iors a d surprisi g  easure e ts. It’s 

related to the formation of localized vortex and arise from a combination of the following facts: 

-

 

The decreased viscosity of liquid at elevated temperatures (for example for water at 40°C the viscosity 
is about half the one at 20°C) 

-

 

The combination of small ID tubing (for example 500 µm ID tubing) with the 1.8 mm ID sensor. 

 

The  transition  from  the  small  ID  tube  to  the  larger  ID  sensor  may  lead  to  a  jet  at  high  flow  speeds  and  low 
viscosity,  see  the  following  sketch.  Such  a  jet  is  inherently  unstable,  which  can  lead  to  strong  fluctuations  at 
high flow-rates and temperatures. The phenomenon is also strongly dependent on the exact  geometry of the 
arrangement.  A  bending  of  the  tube  on  the  inlet  side  may  generate  a  stationary  vortex  and  the  fluid  in 
proximity to the sensor chip may actually be flowing backward, see the next sketch: 

 

 

Содержание FLOW-RATE PLATFORM

Страница 1: ...FLOW RATE PLATFORM User Manual FLOW RATE PLATFORM Version 6A...

Страница 2: ...FLOW RATE PLATFORM 16 6 1 Quick start procedure 16 6 2 Use at high temperature and high flow rate 16 6 3 Use a XL Flow Unit 16 7 CLEANING PROCEDURE 18 8 DUAL CALIBRATION 20 8 1 Principle of single and...

Страница 3: ...ue Will be favored any remote repairs but in case more actions need to be taken the system will come back to Fluigent offices for no additional cost only if it is under warranty The warranty condition...

Страница 4: ...uids from entering the FLOWBOARD this may cause a short circuit failure or other malfunction Failing to respect this advice would Expose you to direct current voltage in case the device is under volta...

Страница 5: ...s flow rate and volume of liquids flowing through your fluidic system The five 5 different FLOW UNIT models offer an extensive choice of flow rate ranges to best match your required precision from 8 n...

Страница 6: ...ture differences thus providing the basic information about the spread of the heat which itself is directly related to the flow rate It is possible to use the Flow Rate Platform with any flow control...

Страница 7: ...ccuracy m v measured value 10 m v above 75 nL min 5 m v above 0 42 L min 20 m v above 4 2 L min 5 m v above 2 4 L min 20 m v above 25 L min 5 m v above 0 04 mL min 20 m v above 0 5 mL min 5 m v above...

Страница 8: ...models the Flow Rate Platform package can contain not always included one kit CTQ_KIT_LQ T o LQ flo u it o e tor for OD tu i g one meter of PEEK Tubing Blue OD ID 1 green sleeve 6 OD 6 adapter PEEK 6...

Страница 9: ...the sides of the device The front of the FLOW UNIT displays information about the range and the calibration The letter i di ates the odel Here it s XL The droplet indicates the calibration Here there...

Страница 10: ...t s L The droplets indicate the calibration Here there are two droplets a blue one and a white one It indicates that the sensor has dual calibration one for water and the other one for isopropyl alcoh...

Страница 11: ...gs and fittings for the different applications that you may use FLUIGENT advises you to make sure that your fluidic connection system fits with the two 2 fluidic ports of the FLOW UNIT If not please n...

Страница 12: ...errules from fittings suppliers to suit your application 4 2 3 How to connect tubing to the FLOW UNIT models The pictures below illustrate how to connect OD tu i g to L a d XL FLOW UNIT odels 1 Cut th...

Страница 13: ...he pictures below show ho to o e t OD tu i g to X a d M FLOW UNIT odels 1 Cut the 1 32 OD tu i g to the desired length leaving a square cut face 2 Slide the fitting over the tubing 3 Insert the assemb...

Страница 14: ...he software cf Flow Rate Platform Soft Front Panel User Manual or Maesflo TM User Manual When using the Flow Rate Platform alone one must use the Flow Rate Platform Soft Front Panel FRP SFP When combi...

Страница 15: ...2 Connection 5 2 1 USB connection Connect the type B plug of the USB cable provided with the Flow Rate Platform into the type B USB port on the front of the FLOWBOARD Connect the other end of the USB...

Страница 16: ...C For example the L Flow Unit model at 50 C has a specified error of 5 30 0 1 8 of the measured value Between 50 C and 80 C the Flow Unit will still be operational and the repeatability will still be...

Страница 17: ...veillance au capital de 141 284 95 Siret 487 636 409 00038 N TVA UE EU VAT Number FR 53 487 636 409 This can lead to a negative flow reading To solve this observed behavior Fluigent advises you to use...

Страница 18: ...llary tube without flushing clean first Also try to avoid letting the filled sensor sit for extended periods depending on your liquid Before storing the sensor always drain of fluid flush with cleanin...

Страница 19: ...on the walls of the glass capillary and form an organic film that can be difficult to remove Flush on a regular basis with solvents such as ethanol methanol or IPA or with cleaning detergents to remov...

Страница 20: ...cohol The FLOW UNIT can be used to handle different fluids not originally calibrated for When possible select a standard calibration field that most closely matches your fluid For example water calibr...

Страница 21: ...rate Note that if a peristaltic or a syringe pump is used one has to wait until the target flow rate is reached settling times can be long and to calculate an average flow rate due to the pulsations...

Страница 22: ...ed characterization of your fluidic system to ork out the relatio ships et ee the MFC pressure ha els a d the flo rate channels This pressure flow rate relation is then used to automatically compute t...

Страница 23: ...FR 53 487 636 409 For OD tu i g To connect our OD tu i g ou eed one nut F 120 and one green sleeve Slide the sleeve over the nut and slide the nut and sleeve over the tubing with the nut thread facin...

Страница 24: ...lters references A 355 A 356 These filters are designed for use with 1 16 OD tubing You can choose either 0 5 m A 700 or 2 m A 701 frit version to filter particles from you flow path Why is the flow r...

Страница 25: ...www fluigent com SA Directoire et Conseil de Surveillance au capital de 141 284 95 Siret 487 636 409 00038 N TVA UE EU VAT Number FR 53 487 636 409 Is it possible to plug the FLOW UNITS directly to th...

Страница 26: ...istance between the FLOW UNIT sides bar 200 100 12 5 Wetted materials Internal Sensor Capillary Material Quartz Glass Fused silica Borosilicate Glass 3 3 Duran Fitting material PEEK polyetheretherketo...

Страница 27: ...ent com SA Directoire et Conseil de Surveillance au capital de 141 284 95 Siret 487 636 409 00038 N TVA UE EU VAT Number FR 53 487 636 409 FLUIGENT BIOPARK 1 mail du Professeur Georges Math 94800 VILL...

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