Access Array System for the Ion Torrent PGM Sequencing System: User Guide
Chapter 1: Access Array System Overview
Before You Begin
15
Before You Begin
IMPORTANT
Before using the Access Array system, read and understand the detailed
instructions and safety guidelines in
Appendix D
.
•
Use good laboratory practices to minimize contamination of samples:
•
Use a new pipette tip for every new sample.
•
Whenever possible, separate pre- and post-PCR activities. Dedicate laboratory
materials to designated areas.
•
Unless otherwise specified, thaw reagents at room temperature (15–30 °C), and then use
them at room temperature.
•
Retrieve only the reagents required from each kit based on the number of IFCs you will
run.
•
Only use the reagents provided in the required kit.
•
Do not swap reagents between kit lots.
•
Mix and centrifuge reagents as directed.
•
Avoid creating bubbles when transferring reagents to the IFC.
•
This protocols in this document require the use of updated scripts for FC1 (v1.6 or later) and IFC
Controller AX (v2.8 or later) that can be downloaded from
fluidigm.com/software
.
