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Performance Results From Evolve®Columns
Performance tests have been performed on the Evolve®range of columns using Toyapearl
Butyl 650M media manufactured by Tosohaas This media type has the particle size 65
micron.
Accepted performance results for this media as quoted in Toyapearl Instruction Manual is
expressed in terms N, number plates per m and asymmetry.
The minimum column efficiencies for Tosohaas Butyl 650M is > 4000 plates per metre with
an asymmetry values should be between 0.8-1.4.
Bed heights of 15-20cm were packed at ambient temperature using filtered deionised water
as the packing buffer. The packed beds were quantified using a 2% acetone solution and the
sample size was equivalent to 0.5cm band width.
The table below shows results when a flowrate of 100cm/hr was used.
Column N, Number of plates Asymmetry
250
5104
1.05
200
4428
1.10
140
4284
1.18
Due to limitations in the chromatography system the Evolve®100mm could only be
quantified with a flowrate of 190cm/hr. The table below gives this result.
Column N, Number of plates Asymmetry
100
4071
1.06
I
Figure A2 Example of typical chromatogram. Evolve®250mm column. 2% Acetone sample,
flowrate 100cm/hr, bed height 19.5cm.
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