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Kits, Spare parts and Accessories

The following kits are supplied with the column:

•

Connection Kit

•

Spares Kit

•

Tool Kit including torque wrench

It is possible to re-order these kits using the product codes listed in the table below.

Connection kit

A kit that contains frequently used connectors is also supplied with the column.

DESCRIPTION

QTY

PRODUCT NUMBER

AA0

7CK

AA1

0CK

AA1

4CK

CONNECTION

KIT

Flow tube Adaptor, 3mm bore

Flow tube Adaptor, 6mm bore

Click Clamp,3/4" Ladish

Flow Tube Seal, Ladish 3.83mm bore

Flow Tube Seal, Ladish 7.0mm Bore

Flowcell locking clip 3mm flowtube

Flowcell locking clip 6mm flowtube

Flow Tube Seal, 4.8mm bore

Flow Tube Seal,7.0mm bore

Adjuster Flow Tube 3mm bore

Adjuster Flow Tube 6mm bore

Chapter

Содержание 100

Страница 1: ...70mm 100mm and 140mm Chromatography Columns ...

Страница 2: ...DELTA PRECISION LTD Chromatography Column OperatingManualandInstallationGuide 70mm 100mmand140mmColumns ...

Страница 3: ...ressure Equipment Directive updated 06 04 2021 E Technical specification updated caster not available on 70 140mm columns 22 04 2021 F Updated to reflect Evolve Trademark status 12 07 2021 Evolve is a registered trademark of Delta Precision Ltd Evolve is a registered trademark in the EU UK and US Manual Revision Date F July 2021 ...

Страница 4: ...te Method 16 Unpacking 18 Cleaning and Storage 18 Maintenance 20 Routine Maintenance Procedures General guidelines 20 Disassembly of 70mm 100mm and 140mm Columns 21 Reassembly of 70mm 100mm and 140 column 24 Troubleshooting 27 Leakage from adjuster seal 27 Leakage from fixed bed support seal 27 Leakage from inlet or outlet 27 Excessive column back pressure 28 Media Leakage 28 Kits Spare parts and ...

Страница 5: ...ation 17 Figure 5 1 Adjuster Assembly Evolve 100 column 22 Figure 5 2 Fixed Assembly Evolve 100 column 23 Figure 5 3 Diagram showing the location of the fixed bed support 24 Figure 5 4 Photograph showing the installation of the fixed bed support 24 Figure 5 4 Photograph showing the location of the adjuster bed support 25 Figure 5 5 Diagram showing the location of the adjuster bed support 25 Figure...

Страница 6: ... other columns available on the market the Evolve columns have an integral bed support and seal assembly The polypropylene mesh and seal are manufactured as a single unit and fits directly on the flowcell without any fixings This has the following advantages Ensure that maximum area is available to flow promoting good chromatographic performance and cleanability Eliminates use of fixings to secure...

Страница 7: ...nd a central height adjust rod This allows the position of the adjuster to be varied using the height adjust rods over the range 20 520mm Tube unit consisting of the column tube flanges and column stand or support legs Fixed assembly consisting of the bottom flowcell assembly and flowtube Figure 1 1 Evolve Column Range 70mm 100mm 140mm 200mm and 250mm columns ...

Страница 8: ...mp 2 30o C Bed support pore size µm 8µm Weights and Dimensions 70 100 140 200 250 Cat No AB070505 AB100505 AB140505 AB200505 AB250505 Adjuster Weight kg 4 5 7 5 12 7 15 Total Weight empty kg 11 5 12 5 19 45 5 52 Total Weight Full kg 13 16 26 60 76 Maximum Diameter ex casters mm 226 226 282 455 495 Maximum Diameter inc casters mm 1 455 495 Maximum height mm 1523 1543 1545 1550 1550 Minimum Clearanc...

Страница 9: ...ent temperature 15 20o C using DI water Figure 1 2 Pressure Drop Evolve 70mm column Figure 1 3 Pressure Drop Evolve 100mm column 0 0 1 0 2 0 3 0 4 0 5 0 6 0 7 0 8 0 500 1000 1500 2000 2500 Linear Velocity cm hr Pressure bar 0 0 2 0 4 0 6 0 8 1 1 2 1 4 1 6 Volumetric Flowrate L min Evolve 70 0 0 1 0 2 0 3 0 4 0 5 0 6 0 7 0 8 0 200 400 600 800 1000 1200 Linear Velocity cm hr Pressure bar 0 0 2 0 4 0...

Страница 10: ...re 1 4 Pressure Drop Evolve 140mm column 0 0 1 0 2 0 3 0 4 0 5 0 6 0 7 0 8 0 9 0 500 1000 1500 2000 2500 3000 Linear Velocity cm hr Pressure bar 0 0 5 1 1 5 2 2 5 3 3 5 4 Volumetric flowrate L min Evolve 140 ...

Страница 11: ...l fixed and adjuster Santoprene Adjuster bed support seal Santoprene Fixed flowcell Polypropylene black Adjuster flowcell Polypropylene black Antijet disc 20 Glass filled polypropylene Sanitary clamp seals Santoprene elastomer Pipe spool Polypropylene Materials of construction non process wetted parts Column Component Material Adjuster flange tube flange and fixed flange fixed flowcell back plate ...

Страница 12: ...ed to room temperature 15º 25ºC Wetted Components Non Wetted Stainless Steel 316L Chemical 1 2 dichloroethane Acetic acid 25 Acetone 5 Acetonitrile Ammonia aqueous 25 Ammonium Sulphate 10 40 Benzyl alcohol 1 Benzyl alcohol 2 Butanol Calcium chloride 2 Calcium hydroxide 30 Calcium hypochlorite Chloracetic acid 50 Chloroform Chromic acid 10 Citric acid Copper sulphate Dichloromethane Dimethyl formam...

Страница 13: ...eroxide Industrial methylated spirit IMS Methanol 20 Methanol 50 Methyl chloride Chloromethane Methyl ethyl ketone MEK Methylene chloride Nitric acid 10 Nitric acid 70 Peracetic acid 300 ppm Phosphoric acid 55 Potassium hydroxide 2M Iso propanol 20 Iso propanol 40 n propanol 20 Sodium acetate Sodium bicarbonate 20 Sodium carbonate Sodium chlorate Sodium chloride 2M Sodium chloride 6M Sodium hydrox...

Страница 14: ...ce within their Operating Instructions there should be no gas e g air present in the columns The substances that will be used in the column will remain in liquid form during the entire operation at the maximum stated operating temperature In addition The maximum operating pressure is greater than 0 5 bar and less than 10 bar The product pressure x volume is equal to or less than 200 bar litres Ove...

Страница 15: ...rovision is made for the use of a hoist It is essential that good practice is adopted regarding the use of appropriate slings and associated equipment Chemical compatibility This manual lists those substances for which the Columns are compatible If substances not listed are to be used the user must consider their effect as the integrity and safety of the Column could be compromised ...

Страница 16: ...allation of bed supports The Evolve range of columns features polypropylene woven mesh bed supports integrated with a Santoprene seal These do not require prewetting before use and are required to be fitted directly onto the adjuster and fixed flowcell All handling of bed supports should be performed by a user wearing gloves Installation of Fixed Bed Support 1 Remove fixed flowcell from the column...

Страница 17: ...of the adjuster unit it is recommended to level the column This is achieved using the spirit level provided in the tool kit and adjustment of the leveling studs located in the foot ring of the column 1 Place the spirit level on the tube flange and adjuster the leveling studs to ensure column is level before proceeding There is approximately 30mm free vertical movement available whilst still mainta...

Страница 18: ...seal into the location groove on the side of the flowcell See Figure 3 3 Figure 3 3 Diagram showing the location of the adjuster bed support 3 Work around the circumference of the flowcell ensuring that seal is correctly fitted See Figure 3 4 Figure 3 4 Photograph showing the location of the adjuster bed support Seal Setting Ring Adjuster Seal Bed Support Seal Smooth Side Bed Support Rough Side Fl...

Страница 19: ...e the adjuster seal is de energized 2 Replace the flange fixing bolts underneath the tube flange and tighten 3 Partially energized the seal and turn the height adjust handles clockwise to set the required bed height 4 Check the column is level by placing the spirit level on the adjuster flange ...

Страница 20: ...etting See table below Column Diameter mm Torque Setting Nm Torque Setting Ft lbf 70mm 20 14 75 100mm 20 14 75 140mm 20 14 75 2 Fill the column with WFI or high quality water and position the adjuster at the operating bed height 3 Ensure the adjuster seal is wetted with buffer or other suitable liquid prior to assembly This helps lubricate the seal to ensure correct seating 4 Activate the adjuster...

Страница 21: ...e slurry into the column Avoid trapping air bubbles in the slurry as it is poured 8 Fill to a maximum of 30mm from the top of the tube 9 Rinse the top inside surface of the column tube to prevent media from becoming trapped between the adjuster seal and the tube 10 Replace the adjuster assembly back into the column tube and secure to the tube flange using the flange fixing bolts Ensure the adjuste...

Страница 22: ...ation 16 Pump buffer into the column at an appropriate rate to pack the bed Ensure fixed end valve is open The packing flowrate should be a minimum of 10 to 20 higher than the highest flowrate to be used during the process Note that some media will pack more ...

Страница 23: ...low adaptor CAUTION Do not adjust the seal actuation knob when the column is under pressure Unpacking The column may be unpacked conventionally that is the adjuster can be removed the bed reslurried and the media poured or pumped out of the column tube Cleaning and Storage Cleaning NOTE Do not use abrasive cleaning materials or pads as these may score plastic components The extensive solvent and c...

Страница 24: ... a cool dry dust free location and protected from light In particular do not expose acrylic column tubes to direct sunlight for prolonged periods or sterilize with ultra violet lights as this may reduce the mechanical strength of the tube ...

Страница 25: ... unpacking columns be careful not to scratch the inside of the column tubes 6 Be careful not to damage stainless steel surfaces by bumping knocking or scratching 7 Do not over tighten components and use correct torque settings as given in this guide 8 Good engineering practices should be followed at all times and operations carried out in an approved and safe manner Tool Where used Allen Key Set V...

Страница 26: ...5 Remove top flowtube adaptor and retain flowtube seal 6 Remove the seal adjust nut and spacer and slide the flowtube and seal adjust tube out of the threaded rod 7 Remove the flowcell locking clip and retain Slide the stainless steel tube off the flowtube 8 Locate the flats on the flowtube and using the correct sized spanner remove the flowtube to expose the flowtube seal 9 The adjuster mesh can ...

Страница 27: ... wrench loosen each of the fixing screws supporting the flowcell with your hand Remove the flowcell and place it on a clean work surface 3 Locate the flats on the process flowtube and using the correct tool remove the flow tube Retain and inspect the flowtube seal 4 To remove the mesh seal assembly from the flowcell carefully prize the bed support seal from the flowcell ...

Страница 28: ... Figure 5 2 Fixed Assembly Evolve 100 column Tube assembly The unit should only be disassembled as apart of an emergency repair procedure Details of the method will be provided with the replacement parts ...

Страница 29: ...k and ensure all connections are secure but do not overtighten Figure 5 3 Diagram showing the location of the fixed bed support Figure 5 4 Photograph showing the installation of the fixed bed support 4 Support the flowcell with your hand and locate fixing bolts Initially hand tighten the bolts working diametrically opposite to ensure equal loading on the seal Then using the fixed end fixing tool a...

Страница 30: ... below Figure 5 4 Photograph showing the location of the adjuster bed support Figure 5 5 Diagram showing the location of the adjuster bed support 2 Push the adjuster seal onto the seal setting ring Ensure that the seal is correctly located by checking it is located in the groove in the setting ring 3 Fit the flowtube seal into the adjuster flowcell and replace the flowtube Use the correctly sized ...

Страница 31: ...lange to assist CAUTION Adjuster units are heavy 7 Locate the flange fixing bolts in the underside of the column tube flange and tighten 8 Replace the flow tube adaptor To ensure the fitting is tightened correctly align the markings on the flow tube and adaptor As shown in Figure 5 7 Initially hand tighten and then use correct size spanner Do not over tighten CAUTION over tightening this adaptor c...

Страница 32: ... adjust the seal actuation knob Leakage from fixed bed support seal Check using a torque wrench that fixed end fixings are set at the correct torque If the column is being operated under cold room conditions or with cold process fluids retighten all bolts If possible empty the column and removed the fixed cell assembly Remove the fixed bed support with integral seal and inspect Ensure that the sea...

Страница 33: ... to the column are correctly functioning Rectify any issues that are observed and repeat the pressure flow test If process feedstreams are highly viscous or contains particulates or may precipitate it is possible that the bed supports have become blocked This can also occur if the chromatographic media contains fines If this is suspected replace the bed supports and repeated the pressure flow test...

Страница 34: ...rs is also supplied with the column DESCRIPTION QTY PRODUCT NUMBER AA07CK AA10CK AA14CK CONNECTION KIT Flow tube Adaptor 3mm bore 1 X X Flow tube Adaptor 6mm bore 1 X Click Clamp 3 4 Ladish 3 X X X Flow Tube Seal Ladish 3 83mm bore 6 X X Flow Tube Seal Ladish 7 0mm Bore 6 X Flowcell locking clip 3mm flowtube 1 X X Flowcell locking clip 6mm flowtube 1 X Flow Tube Seal 4 8mm bore 6 X X Flow Tube Sea...

Страница 35: ... X Spares Kit DESCRIPTION QTY PRODUCT NUMBER AA07SK AA10SK AA14SK SPARES KIT Bed Support Assembly 70 Fixed 1 X Bed Support Assembly 70 Adjuster 1 X Bed Support Assembly 100 Fixed 1 X Bed Support Assembly 100 Adjuster 1 X Bed Support Assembly 140 Fixed 1 X Bed Support Assembly 140 Adjuster 1 X Adjuster seal Evolve 70mm 1 X Adjuster seal Evolve 100mm 1 X Adjuster seal Evolve 140mm 1 X Flow Tube Seal...

Страница 36: ...R AA07ESK AA10ESK AA14ESK ELASTOMER SEAL KIT Adjuster seal 70mm Column 1 X Adjuster seal 100mm Column 1 X Adjuster seal 140mm Column 1 X Flow Tube Seal Ladish 3 83mm Bore 3 X X Flow Tube Seal Ladish 7 0mm Bore 3 X Flow Tube Seal 4 8mm Bore 3 X X Flow Tube Seal 7 0mm Bore 3 X DESCRIPTION QTY PRODUCT NUMBER AA07FCK AA10FCK AA14FCK ADJUSTER CELL KIT Adjuster Cell 1 X Adjuster Cell 1 X Adjuster Cell 1...

Страница 37: ...Pipespool Elbow 140mm Column 1 X Flow Tube Seal Ladish 3 83mm Bore 3 X X Flow Tube Seal Ladish 7 0mm Bore 3 X Bed Support Assembly 70 Fixed 1 X Bed Support Assembly 70 Adjuster 1 X Bed Support Assembly 100 Fixed 1 X Bed Support Assembly 100 Adjuster 1 X Bed Support Assembly 140 Fixed 1 X Bed Support Assembly 140 Adjuster 1 X Anti Jet Disc Small 2 X X X DESCRIPTION QTY PRODUCT NUMBER AA07TRK AA10TR...

Страница 38: ... Ladish 3 83mm Bore 3 X X Flow Tube Seal Ladish 7 0mm Bore 3 X Bed Support Assembly 70 Fixed 1 X Bed Support Assembly 70 Adjuster 1 X Bed Support Assembly 100 Fixed 1 X Bed Support Assembly 100 Adjuster 1 X Bed Support Assembly 140 Fixed 1 X Bed Support Assembly 140 Adjuster 1 X Anti Jet Disc Small 2 X X X Adjuster Cell 70 1 X Adjuster Cell 100mm 1 X Adjuster Cell 140mm 1 X Flowcell locking clip 7...

Страница 39: ...mm Dia X 1m 2 6mm Tee 2 Click Clamp 3 4 Ladish 10 Flow Tube Seal Ladish 7 0mm Bore 10 Blanking Cap 2 DESCRIPTION QTY Product Number FLEXIBLE HOSE KIT 70 100 Flexible Hose 3mm Dia X 2m 2 AAFHK1 Flow Tube Seal Ladish 3 83mm Bore 2 Click Clamp 3 4 Ladish 2 DESCRIPTION QTY Product Number FLEXIBLE HOSE KIT 140 200 250 Flexible Hose 6mm Dia X 2m 2 AAFHK2 Flow Tube Seal Ladish 7 0mm Bore 2 Click Clamp 3 ...

Страница 40: ...35 Contact Us To view our Evolve Column User guides please visit www astreabioseparations com resources evolve column user guides For further enquires please contact sales astrea bio com Chapter 8 ...

Страница 41: ...tion is used then the UV absorbance is measured and plotted If NaCl solution is use then conductivity is measured and plotted The plot obtained is then analyzed to calculate the following terms of efficiency Chromatographic efficiency is expressed as HETP height equivalent to theoretical plate This is calculated direct from the UV plot as shown in the diagram below Calculation of HETP HETP Where L...

Страница 42: ...e diameter Dilution factor includes the initial sample volume in the calculation This allows comparison between different peak shapes The dilution factor is given by the following equation Where the initial sample volume and the values of a and b are all expressed in the same volume units Comparison of results should be undertaken with caution Different media manufacturers often express performanc...

Страница 43: ...1 4 Bed heights of 15 20cm were packed at ambient temperature using filtered deionised water as the packing buffer The packed beds were quantified using a 2 acetone solution and the sample size was equivalent to 0 5cm band width The table below shows results when a flowrate of 100cm hr was used Column N Number of plates Asymmetry 250 5104 1 05 200 4428 1 10 140 4284 1 18 Due to limitations in the ...

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