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Polymerase Chain Reaction Cabinets

LAYOUT A

DOMINIQUE

DUTSCHER

SAS

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Содержание Airstream PCR-3A

Страница 1: ...safe use of your Esco products Esco Technologies Inc 903 Sheehy Drive Suite F Horsham PA 19044 USA Tel 215 441 9661 Fax 484 698 7757 escolifesciences us eti admin escoglobal com Rest of World Esco Mi...

Страница 2: ...Airstream are registered trademarks of Esco Material in this manual is provided for informational purposes only The contents and the product described in this manual including any appendix addendum a...

Страница 3: ...ilter 15 1 4 1 SCR A 16 1 4 2 PCR A 17 1 5 Replacement of Blower 17 1 5 1 SCR A 18 1 5 2 PCR A 19 1 6 Fluorescent Lamp and UV Lamp Replacement 19 1 6 1 Replacing the fluorescent lamp 19 1 6 2 Replacin...

Страница 4: ...UNIT FOR IMPLEMENTATION B 13 Aug 2019 Updated certification testing Added manual revision history C 20 Sep 2019 Updated Total airflow for HEPA filter leak test D 09 Jun 2020 Removed airflow and sash...

Страница 5: ...modifications to the product Consumables Esco is not liable for any damage incurred on the objects used on or stored in Esco equipment If the objects are highly valuable user is advised to have in pla...

Страница 6: ...D O M I N I Q U E D U T S C H E R S A S...

Страница 7: ...Ultra low Temperature Freezer 5 years limited 5 years on compressor Laboratory Centrifuge 2 years limited Freeze Dryer 2 years limited Laboratory Refrigerator 2 years limited Thermal Cyclers 2 years...

Страница 8: ...be used in the cabinet unless adequate risk assessment has been carried out Before you process you should thoroughly understand the installation procedures and take note of the environmental electric...

Страница 9: ...r recycling disposal Recommended method of disposal is according to The Federal State and Local Government regulations Directive 2002 95 EC on Restriction on the use of Hazardous Substances RoHS With...

Страница 10: ...s amending directives 2014 30 EU The Electromagnetic Compatibility Directive and its amending directives 2011 65 EU The RoHS in Electrical and Electronic Equipment Directive and its amending directive...

Страница 11: ...es 2014 30 EU The Electromagnetic Compatibility Directive and its amending directives 2011 65 EU The RoHS in Electrical and Electronic Equipment Directive and its amending directives has been designed...

Страница 12: ...nets generally require Re certification when o The cabinet is relocated o Decline of cabinet performance is suspected o After filter or blower replacement o At least once a year During recertification...

Страница 13: ...D O M I N I Q U E D U T S C H E R S A S...

Страница 14: ...Polymerase Chain Reaction Cabinets 2 1 1 Certification Flowchart D O M I N I Q U E D U T S C H E R S A S...

Страница 15: ...the Motor Voltage Sampling Port Use the multimeter to take the motor voltage with corresponding air velocityreading Adjust the airflow by adjusting the speed controller Use the knob provided to adjus...

Страница 16: ...ocity must be more than 0 275 m s or 54 fpm Actual motor voltage for nominal airflow True RMS VAC Averaging VAC Testing was performed in accordance with the following IEST Institute of Environmental S...

Страница 17: ...cabinet model 2 feet Total Airflow 177 Airflow Filter Leaks detected in media Yes No Leaks detected in gasket Yes No Particle penetration Acceptance Maximum particle penetration of 0 01 for filters no...

Страница 18: ...larger sampled in 0 028 m3 ofair Cleanliness rating ISO Class 3 as per ISO 14644 1 35 count for particles equal to and larger than 0 5 m per 1 m3 or 35 3 ft3 ofair Results Pass Fail 1 3 1 4 Light Int...

Страница 19: ...t to Back Distance from walls b mm in 265 10 4 265 10 4 Average light reading lux ft candles Acceptance Average light reading should be at least 800 lux 75 ft candles Results Pass Fail Acceptance crit...

Страница 20: ...ined with transient ambient noise may vary the corrected noise level by 1 5 dBA Testing was performed in accordance with the following IEST Institute of Environmental Sciences and Technology contamina...

Страница 21: ...rom walls b mm in 150 5 9 150 5 9 Distance apart y mm in 157 6 2 157 6 2 Average Velocity m s fpm Maximum Deviation m s fpm Acceptance 0 275 0 325 m s 55 65 fpm Deviation Acceptance 20 Result Pass Fai...

Страница 22: ...314 12 4 619 24 4 Front to Back Distance from walls b mm in 150 6 150 6 Distance apart y mm in 157 6 2 157 6 2 Average Count for particle size of 0 5 micron and larger sampled in 0 028 m3 ofair Cleanl...

Страница 23: ...266 354 Record the concentration below 3 Connect the aerosol generator to compressed air or compressed nitrogen supply Open the aerosol generator valve s Set the supply pressure valve to 23 psi for P...

Страница 24: ...e apart x mm in 330 12 9 635 25 Front to Back Distance from walls b mm in 265 10 4 265 10 4 Average light reading lux ft candles Acceptance Average light reading should be at least 975 lux 91 ft candl...

Страница 25: ...was done in the factory that is susceptible to reflective reverberant and background noise effect This noise figure may be different than the brochure value that is obtained from anechoic chamber 4 If...

Страница 26: ...n 530 20 9 530 20 9 Left to Right Distance from walls a mm in 150 5 9 150 5 9 Distance apart x mm in 315 12 1 312 12 Average reading W cm2 Acceptance UV Radiation intensity inside the cabinet should n...

Страница 27: ...dremove the perforatedshelf 3 Unscrew andremove the UV lamp and unsnap the holder 4 Unbolt andremove the filter clamp Makesureto hold the filter when doing this 5 Carefullyremove the filterand replace...

Страница 28: ...ew andremove the perforatedshelf 3 Unscrew andremove the UV lamp and unsnap The holder 4 Unbolt andremove the filter clamp Ensure to hold the filter when doing this 5 Carefullyremove the filterandrepl...

Страница 29: ...1 Remove the blackfoam at topexhaust 2 Unscrewthe top metal mesh andremove 3 Removethe blowerwiring connection 4 Unscrewthe blower mounting house andtake out 5 Replace the blower andreverse abovesteps...

Страница 30: ...move the black foam at top exhaust 2 Unscrewthe top metal mesh andremove 3 Disconnectthe blowerwiring 4 Unscrewthe blower mounting house andtake out 5 Replace the blower andreverse abovesteps 6 Re cer...

Страница 31: ...bulb steady 4 Remove the bulbs from the mounting clips and replace with new ones 5 Replace the power clips on the ends of the new bulbs and ensure they are firmly seated 6 Reconnect the cabinet to the...

Страница 32: ...all building socket Fig 1 1 Wall socket Check whether power cord has been connected properly into wall building socket and also into theunit Cabinet does not Power cord is not connected properly or fa...

Страница 33: ...ped condition Locate electrical panel at the top right portion of the cabinet with red metal cover Open electrical panel and ensure cabinet is plugged in to main power supply Cabinet does not Carefull...

Страница 34: ...D O M I N I Q U E D U T S C H E R S A S...

Страница 35: ...the cord is connected The input of SMPS should be 230 VAC 10 for PCR XA1 3 or 115 VAC 10 for PCR XA2 4 If input is correct but output is not then replace the SMPS Cabinet does not start LCD button fa...

Страница 36: ...damage If these conditions DO NOT exist proceed to next step Fig 1 10 Cabinet does not See Layout A at the end of this section to locate the relay board inside electrical panel Measure the incoming vo...

Страница 37: ...all fuses on relay board Turn off power by unplugging the cabinet from the main supply Remove fuses and physically inspect or check continuity Cabinet does not Turn the cabinet on by connecting to the...

Страница 38: ...witching the rocker switch to I or on position at control panel see Fig 2 2 If the FAN does not come on check for loose or improper wiring connections at the back of theswitch If the problem persists...

Страница 39: ...this section to locate the motor speedcontroller Add a jumper wire between the 2 cables going to speed control see Fig 2 4 2 5 Plug the cabinet to the main supply If the fan operates properly the spee...

Страница 40: ...capacitor cables Correct values are shown in Table 2 below If the value is out of given ranges replace it If the capacitance is within the given ranges proceedto next step WARNING The capacitor may s...

Страница 41: ...the NO to Common and NC to Common contactsare correct proceed to the next step For SCR PCR Check K1 relay See Layout A at the end of this sectionto locate the K1 relay With cabinet still switched off...

Страница 42: ...there isexcessive heat in the cabinet If the FAN does not restart proceed to the nextstep Motor failure Unplug the cabinet from the main supply Check motor condition If physical damage is present lik...

Страница 43: ...blockage interference switch ON the blower fan Open the top access cover and locate electricalpanel inside Refer to Fig 3 1 PCR or Fig 3 2 SCR below tolocate motor voltage sampling port Using voltmet...

Страница 44: ...r voltage For new cabinets if the motor voltage is lower than the Nominal Voltage value recorded in the factory test report adjust the speed control to get output voltageto blower as specified in the...

Страница 45: ...ded it s time to change the filter Instruction on how to replace the filter is available in this manual under ServiceSection If filter is not loaded proceedto next step Fluctuating Voltage Measureand...

Страница 46: ...gone measurethe actual airflow velocity If the velocity is still in the acceptable range keep it If velocity is out of acceptable rangeand noise still persists proceed to the next step Loosed motor or...

Страница 47: ...the mainsupply Open the top access cover and locate electricalpanel behind it Open electricalpanel and see Layout A at the end of this section to locate the main board inside electricalpanel Check wh...

Страница 48: ...es PIN number 1 Interface board and membrane keypad are located behind the blue panel underneath the light metaldeflector With the cabinet still turned off uninstall the fluorescent lightand metaldefl...

Страница 49: ...inets 36 Inoperative buttons Fig 7 4 Defective Cable and or Interface Board and or keypad Replacethem one by one to check which one s among them is aredefective Replace the defectivepart s D O M I N I...

Страница 50: ...ne keypad Check AC voltage at ballast input between pin NO onJ13 terminal on relay board to neutral see Fig 8 1below It should be 230 VAC 10 for 230 V cabinetsor 115VAC 10 for 115 V cabinets If not ch...

Страница 51: ...ectionbetweenlight switch fluorescent lamp ballast and female connectorB For connector B disconnect the male side from electrical panel then check male and female side connectors for any loose or wron...

Страница 52: ...efer to the Magnetic Switch troubleshootingsection Switch on the UV lamp by pressing the UV button see Fig 9 2 below If UV MODE is displayed on LCD but UV is not on after pressing UV button please ref...

Страница 53: ...ave a problem For checking the connection follow nextstep For SCR Turn off the unit Use a continuity tester across pin connector A7 and A8on themaleconnector Pleaseensure thatthehingedwindowis fully c...

Страница 54: ...linside See Layout B at the end of this section to locate the UV ballast and connector B inside electricalpanel Turnonthecabinetbyconnectingtothemainsupply then switchonthe UVbyturningUV timeron Makes...

Страница 55: ...de from electrical panel then check male and female side for any bad connection Check also connection of the UV lamp holder see Fig 7 3 For SCR Unplug the cabinet from the mainsupply Open the top acce...

Страница 56: ...mally Open to Common doesn t have continuity while the Normally Closed to Common has continuity If the NO to Common and NC to Common contacts are not correct replace the RelayBoard For SCR Unplug the...

Страница 57: ...44 Polymerase Chain Reaction Cabinets LAYOUT A D O M I N I Q U E D U T S C H E R S A S...

Страница 58: ...45 LAYOUT B D O M I N I Q U E D U T S C H E R S A S...

Страница 59: ...aked epoxy polyester powder coated finish Coated with Esco ISOCIDE antimicrobial coating Work Zone 1 2 mm 0 05 18 gauge stainless steel grade 304 Electrical Model SCR 2A1 220 230 VAC 50 Hz SCR 2A2 110...

Страница 60: ...47 D O M I N I Q U E D U T S C H E R S A S...

Страница 61: ...48 APPENDIX D O M I N I Q U E D U T S C H E R S A S...

Страница 62: ...blems encountered etc 2 Any decontamination procedure performed by either the user or the technician should be recorded down as well Please also record any major maintenance procedure performed by the...

Страница 63: ...gy Illinois USA BS EN 13091 Biotechnology Performance Criteria for Filter Elements and Filtration Assemblies 2000 British Standards UK CLEAN AIR TECHNOLOGY ISO 14644 Cleanrooms and Controlled Environm...

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