Rev B 2022/03
55
CyBio Well vario
Initial Start-Up & Routine Start-Up Procedure
6.3.3
Leak Test
Leak testing is required, in order to make sure that the pipettor head is free from points of
leakage. Subject to testing are the pistons, the pipette tips and the silicon membrane.
Testing is performed through aspiration of a certain volume of dye solution into the pipet-
ting tips and visually tracking the level of liquid over a time of two hours (96 wells) or using
a different procedure for 384 well pipetting heads, which is described further below.
Test sequence:
Make sure the system is in a horizontal position. Carefully place a spirit level onto the
transport rails for this purpose. Use setting screws for horizontal adjustment as neces-
sary.
Remove old tips.
Thoroughly inspect silicon membrane for cracks or sedimentation, using a suitable light
source. Carefully clean the silicon membrane using a dust-free, slightly moistened piece
of cloth. Exert only moderate pressure, in order to prevent unwanted shifting of the
membrane. Use caution to prevent sedimentation remaining in the membrane open-
ings as you wipe the membrane clean. Allow the membrane to dry.
Install tip magazine with new tips and clamp the tips.
Bring the reagent reservoir into position under the tips.
Raise the reagent reservoir to tips level – the pipette tips should submerge by at least
2 mm below the surface of the test solution.
Moisten the pipette tips on the inside by performing five rinse cycles.
Aspirate 50 % (of end volume) of dye solution.
Visually check the liquid level of all pipette tips over at time of two hours. Document all
tips with a non-conforming liquid level.
Visual testing may be hard to perform with 384-well/25
μ
l pipette tips. In cases like
this, you should follow a procedure similar to
: After half an hour,
completely dispense the nominal volume into a 384 well microplate with flat transpar-
ent bottom containing prefilled buffer solution and measure extinction in the vertical
photometer. The volume dispensed per pipetting tip should not deviate from the mean
value by more than 20 %.
Result of leak testing
CyBio Well vario 96
There must be no perceivable difference in the filling height of the various pipetting tips on
completion of testing.
CyBio Well vario 384/CyBio Well
vario1536
There must be no individual variances
≥
20 % on completion of testing.
A drop in the fill level of a tip suggests a potential leak in this tip. First clean the sealing
mat (remove dust, etc.). Repeat this test with new pipette tips. If the leak is found to occur
in the same place, you should contact the manufacturer or your service partner.
TIP
Keep the pipette tips submerged during leak testing, in order to prevent formation of
droplets at their ends, because droplets may falsify the result of testing.
Содержание Analytik Jena CyBio Well vario
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