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4

FEATURES OF THE SENTRY

®

 200 INSTRUMENT

The SENTRY

®

 200 is a portable instrument used for specific diagnostic tests known as fluorescence polarization 

assays. This instrument has been designed to provide an easy and accurate method for performing tests on site as 

well as in a laboratory setting. The SENTRY

®

 200 can be used to perform one test (single assay) or a single type of test 

for a larger group of samples (batch assay).

The SENTRY

®

  200  is  a  single  well,  fluorescence  polarization  instrument.  All  necessary  optics,  electronics  and 

computing capabilities are included within the instrument.  

DO NOT OPEN THE INSTRUMENT HOUSING OR REMOVE ANY PARTS.  Opening the instrument housing may void 

the warranty.  Do not attempt to disassemble or modify the instrument. There are no user serviceable parts inside 

the instrument. This machine contains high voltage components. Never attempt any maintenance procedure not 

described in this manual. Incorrect maintenance procedures may damage instrument or cause electrical shock.

Please allow Ellie to perform all maintenance, repairs and servicing.   

Electrical Power 

The SENTRY

®

 200 may be powered by plugging the provided 

power  adapter  into  a  wall  outlet  (100-240  volts,  50-60Hz). 

Adapters for different types of wall plugs are included with the 

device.  The instrument may also be powered by a battery or by 

the car cigarette lighter adapter.  

Operating Environment

The SENTRY

®

 200 has been designed as a portable instrument 

appropriate for both on site and laboratory use.  Designed 

for  field  conditions,  the  SENTRY

®

 200 should perform in all 

reasonable environments, provided that the internal optics 

remains clean and dry.  

The instrument will function under environmental 

temperatures  between  39o  F  and  115o  F  (4o  C  and  46o  C).  

 

The SENTRY

®

 200 is neither water resistant nor waterproof.  It 

should not be submerged in any liquids.  The exterior of the 

instrument housing may be cleaned with a soft, damp cloth.

Always prepare a sample before inserting it into the 

 

SENTRY

®

  200.    NEVER  PIPETTE  INTO  A  TUBE  WHEN  IT 

IS  INSIDE  THE  SENTRY

®

 200.  Spilling liquid or breaking 

glass inside the instrument’s optic chamber will damage the 

SENTRY

®

 200 and likely require repair.  

NEVER TRANSPORT THE SENTRY

®

 200 WITH A TEST 

SAMPLE TUBE INSIDE IT. WHEN FINISHED RUNNING 

SAMPLES ALWAYS REMOVE THE TEST TUBE AND CLOSE 

THE TOP OF THE OPTICS CHAMBER TO PREVENT THE 

ACCUMULATION OF DUST. 

Always use clean glass 10 or 12 mm by 75 mm borosilicate test 

tubes with the SENTRY

®

 200. 

Содержание SENTRY 200

Страница 1: ...1 F L U O R E S C E N C E P O L A R I Z A T I O N I N S T R U M E N T S S e n t r y 2 0 0 M A N U A L ...

Страница 2: ...ow to move between menu items This document has been prepared for the use of the customers and personnel of Ellie LLC The information contained in this manual is proprietary The manual may not be copied reproduced translated or transmitted in any form without the express written permission of Ellie While Ellie has made every effort to produce an accurate and useful manual no representation or warr...

Страница 3: ... SENTRY 200 10 TESTING WITH SENTRY 200 11 Main Screen 11 Run Assay 11 Running Fluorescence Polarization Assay 12 DATA MANAGER 14 Data Management Screen 14 Viewing Test Data on the SENTRY 200 Screen 14 Erasing Data 15 Available Memory 15 DATA TRANSFER 16 Installation of Sentry Tools on Personal Computer 16 Using the Assay Retrieval Tool 17 Using the Sentry Commander AddIn 18 TROUBLESHOOTING 20 ELLI...

Страница 4: ...lugging the provided power adapter into a wall outlet 100 240 volts 50 60Hz Adapters for different types of wall plugs are included with the device The instrument may also be powered by a battery or by the car cigarette lighter adapter Operating Environment The SENTRY 200 has been designed as a portable instrument appropriate for both on site and laboratory use Designed for field conditions the SE...

Страница 5: ...for 1 2 for 2 3 for 3 etc Alphabetical characters can be entered by depressing the corresponding key repeatedly to cycle through the associated numbers and letters For example the screen will display the lower case letter n by depressing the 6 key three times cycling through the following entries 6 m n o M N O Press the right or left arrow keys to move to the adjacent space to enter the next lette...

Страница 6: ...e Select button below the screen to enter the Setup screen Setup Screen The Setup screen contains several entries that cannot all be displayed on the same screen indicated by the down arrow in the upper right corner of the LCD Pressing the down arrow key when you have reached the last line of the first screen will display the second screen On the first screen Time Date Contrast and PMT entries are...

Страница 7: ... The displayed value is the amount of time in half of seconds instrument readings are performed This value should never be less than 10 Figure 4 Bluetooth Your SENTRY 200 reader is equipped with Bluetooth to allow for easy data transfer from the reader to other Bluetooth enabled devices The Bluetooth menu allows you to setup the device for Bluetooth communication The Bluetooth menu contains four s...

Страница 8: ...can take a long time to complete the SENTRY 200 will not enter search mode automatically Click on the Select option button to start the search Figure 6 When the search is complete you will be prompted with a new screen If there are no Bluetooth devices in range you will see a screen with No Devices Found Figure 7 Please note that Bluetooth range for this device is 12m 39 ft If new devices are foun...

Страница 9: ...urn off Bluetooth support see Bluetooth setup pg 7 for BT Mode Figure 9 Pairing failed error If an error occurs during pairing you will be presented with a screen showing Pairing failed error Figure 9 When finished press the Back button to return to the Setup screen ...

Страница 10: ...e reading for the instrument and adjust the G factor The Medium Polarization Standard provides information about instruments range in the most used part of the reading spectrum An optional High Polarization Standard is also available for use in confirming the instrument s full range of polarization detection For calibration instructions refer to the insert that was supplied with your standards kit...

Страница 11: ...end of the entry if it exceeds nine characters in length 4 Press the Done key Test Type SENTRY 200 is equipped to measure Fluorescence Polarization Assay FPA in Batch mode only To input the Technician Name optional 1 Use the up or down arrows to move to select the Tech line 2 Press the Edit key 3 Use the alpha numeric keyboard to enter the name of the individual or technician performing the test u...

Страница 12: ... Use the up or down arrows to move to select the Sample line 2 Press the Edit key 3 Use the alpha numeric keyboard to enter an appropriate Sample using the text entry procedures The Sample would usually be some form of unique sample or animal identification An asterisk will appear at the end of the entry if it exceeds seven characters in length 4 Press the Done key To measure Blank Intensity in Ba...

Страница 13: ...will be automatically selected 7 Continue reading samples as previously described To Review Test Results 1 Use left and right arrow keys to move between samples 2 Use up and down arrow keys to move between three screens for each sample which display all relevant results see Figure below 3 SENTRY 200 is providing following information for each sample Blank Blank Tracer Tracer Sample mP To Print Tes...

Страница 14: ... down arrows to move to select the View Data line 2 Press the View Print data key to move to the View Print screen a Use the up or down arrows to scroll through the initial View Print screen to the second and third View Print screens b The initial View Print screen displays the Test Name Test Type Tech and Lot entries for the current sample c The second View Print screen displays the Location Time...

Страница 15: ...a by selecting the Yes key Selecting the No key returns you to the Data Management screen Available Memory The available memory remaining to store additional test results is displayed on the Avail Memory line of the Data Management screen Results from approximately 30 000 prior tests are retained in the memory unless erased or overwritten Warning Download data to a personal computer and then erase...

Страница 16: ...uired for Sentry Tools installlation User should be allowed to write to ProgramFiles AppData and C Sentry 200 folder Please consult your System administrator for help Installation of Assay Retrieval Tool Program on a Personal Computer 1 Insert the Sentry Tools CD into the personal computer The computer s operating system must be Windows XP or above You can also download the software from the Inter...

Страница 17: ...our device default PIN code is 1234 After you click on the Close button your device should be installed In case of problems with driver installation please contact your local computer administrator for help 14 After the driver installation for your SENTRY 200 instrument double click on your instrument in Devices and Printers Select the Hardware tab and write down the serial port assigned to your i...

Страница 18: ...configuration of your device pg 7 and perform pairing of your SENTRY 200 device with personal computer pg 16 Data position The Data position drop down menu alows you to select the way in which data returned from device is displayed Available options are Horizontal data is displayed in next column and Vertical data is displayed in next row Set Device Date The Set Device Date And Time button allows ...

Страница 19: ... shown in Microsoft Excel status bar Data is displayed in cells according to selected Data position field in configuration see the Data position on pg 18 Sentry Commander AddIn is providing raw data from SENTRY 200 instument It is users responsibility to write functions that are going to calculate polarization value from this data ELLIE LLC is going to provide sample Microsoft Excel worksheet to u...

Страница 20: ...nge to 0 001 Recalibrate the instrument and retest pg 10 Blank Intensity 0 Assay Time set to 0 Go to Setup menu and set Assay Time PMT voltage set to value less than 80 Go to Setup menu and set the PMT value Lamp out PMT not working Main board error Flip open the lid and press Read Check to see if the lamp turns on and off If the intensity is still zero the unit needs servicing Sample Intensity 0 ...

Страница 21: ...nt Power Requirements 9 12V DC 250 mA Dimensions Width 4 2 inches 107 mm Length 8 0 inches 203 mm Height 2 2 inches 56 mm 1 7 inch tube stack Weight 2 7 pounds 1 1 kg Filters Excitation 485 nm Emission 535 nm Tube Format Size 10mm or 12mm x 75 mm borosilicate glass Precision 0 5 mP standard deviation at 1 nM fluorescein Measurement Time 7 seconds minimum Case Hard cover carrying case available Pro...

Страница 22: ...strument voids this Warranty THE PURCHASER S SOLE AND EXCLUSIVE REMEDY AGAINST Ellie shall be for the repair of replacement of defective original parts after it is determined by Ellie that the parts are indeed defective In addition the above warranty does not cover any costs connected with removing or replacing any defective part or the costs to transport the defective part or instrument back to t...

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