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AEROSPRAY

®

TB

MODEL 7722

Applications
Manual

SLIDE STAINER/CYTOCENTRIFUGE

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Содержание 7722

Страница 1: ...AEROSPRAY TB MODEL 7722 Applications Manual SLIDE STAINER CYTOCENTRIFUGE...

Страница 2: ...ELITechGroup Inc 370 West 1700 South Logan Utah 84321 8212 USA 800 453 2725 1 435 752 6011 WWW ELITECHGROUP COM...

Страница 3: ...AEROSPRAY TB SLIDE STAINER CYTOCENTRIFUGE Model 7722 Applications Manual 57 2004 01E...

Страница 4: ...emarks of ELITechGroup Inc Other trade names used in this manual are trademarks of their respective owners used here for information only ELITechGroup Inc makes no express or implied warranty regardin...

Страница 5: ...nance Function Keys 23 Section 2 Setup and Preparation for Operation 2 1 Instrument Setup 24 Unpacking and Installing the Stainer 24 Connecting the Drain Tube and Waste Container 25 Connecting Power 2...

Страница 6: ...ner 4 1 Operating Instructions 48 Suggested Staining Protocol 48 Performing a Hub Pattern Test 48 Figure 7 Correct Hub Pattern Test Result 48 Figure 8 Incorrect Hub Pattern Test Result 48 Loading the...

Страница 7: ...rming the Slide Pattern Test 71 Figure 14 Correct Slide Pattern Test Result 71 Figure 15 Incorrect Slide Pattern Test Result 71 Section 7 Solving Problems 7 1 Troubleshooting 72 Table 12 General Troub...

Страница 8: ...er Wash Setting 98 Counterstain Setting 98 Counterstain Wash Setting 98 Dry Time Options 98 Table A 12 Slide Carousel Timing 98 Table B 30 Slide Carousel Timing 99 Appendix E Sample Slide Preparation...

Страница 9: ...e CE Declaration of Conformity included with the documents shipped with this device Understanding Warnings This manual uses three warning levels to alert you to important information as shown in the f...

Страница 10: ...scribed in the IEC 61326 series CAUTION To avoid serious instrument damage never use reagents other than those supplied for this model stainer by Elitech Group Except for deionized or distilled water...

Страница 11: ...gent Stain sequence programmability Reagent and waste level monitoring Log files The correct accessory must be used for each function The Cytopro Cytocentrifuge Rotor is available as an option offerin...

Страница 12: ...h Screen Controls Menu driven icons Drain Connection Connector on rear panel accepts male connector attached to vinyl drain tube 1 8 meters 6 ft length supplied Ventilation Air is exhausted from the s...

Страница 13: ...r B Counterstain C Primary Stain fluorescence or carbol fuchsin D Deionized or distilled water NOTE Deionized or distilled water is not provided by Elitech Group and must be obtained locally Water sho...

Страница 14: ...pm Maximum Capacity Maximum Sample Volume Elitech Group 12 Slide Carousel AC 188 950 rpm 5 12 each 26 mm x 76 mm 1 x 3 inch microscope slides N A Elitech Group 30 slide Carousel AC 189 950 rpm 5 30 ea...

Страница 15: ...uctions For Use CE Mark product meets the essential requirements designated in Annex I of the In Vitro Device Directive IVDD 98 79 EC Consult Instructions For Use Do Not Reuse Do not use if package is...

Страница 16: ...ber Temperature Limitation indicates high and low limits Normal room temperatures are specified for all Elitech Group Reagents Use By Volume Control General Warning Caution Risk of Danger Warning Biol...

Страница 17: ...30 slide Carousel 2 Front Panel with Touch Screen Display 3 Bowl 4 12 slide Carousel 5 Lid with Safety Lock 6 Right Side Panel with Label Indicating Reagent Positions A Decolorizer B Counterstain C Pr...

Страница 18: ...terface Section 1 3 for more information Figure 3 Rear Panel 1 Level Detect Connection for Reagent D deionized or distilled water 2 USB Ports 3 Network Ethernet Connection 4 Exhaust Vent 5 Rear Panel...

Страница 19: ...onized or Distilled Water front 2 Nozzle A Decolorizer 3 Nozzle B Counterstain 4 Nozzle C Primary Stain 5 Nozzle E Approved Alcohol 6 Nozzle DR Deionized or Distilled Water rear 7 Drive Hub Figure 5 N...

Страница 20: ...cked pumps Silicon Grease Lubricates the nozzle threads for easy assembly Nozzle Wire Cleans nozzle housing orifices Nozzle Cleaning Strainer Holds nozzle parts to prevent loss during cleaning Nozzle...

Страница 21: ...Cleaning Solution Barcode Reader An optional barcode reader is available for use with the Aerospray TB Stainer Cytocentrifuge Model 7722 Figure 6 Barcode Reader Other Needed Items The following parts...

Страница 22: ...e Test and Line Flush functions Clean Performs the Clean cycle Cyto Enters the Cytocentrifuge mode System Information Shows the system information including serial number and software version Allows a...

Страница 23: ...to create edit and delete stain programs Cyto Programs Allows users to create edit and delete cytocentrifuge programs Reagents Allows users to edit reagent information Users Allows users to create and...

Страница 24: ...ogramming mode for creating staining and cytocentrifuge programs Also allows the system administrator to authorize new users Allows manual entry of slide or specimen information Delete Erase Remove De...

Страница 25: ...de Pattern or Hub Pattern Test to ensure nozzles are clear of debris and spraying properly Volume Test Performs Volume Test to verify the selected nozzle volume is within the correct range Line Flush...

Страница 26: ...ern and Spray Volume tests CAUTION Contact Elitech Group before installing the instrument if you observe any damage to the packaging or equipment 1 Unpack and inspect the instrument 2 Check that the c...

Страница 27: ...s and is as straight and as short as possible Cut off excess tubing as needed 3 Connect the drain tube to the waste container If using a waste bottle with level detect AC 182 4 Connect the waste monit...

Страница 28: ...Appendix D and cleaning certain reagent lines CAUTION When switching between carbol fuchsin and fluorescence staining the user must perform a B Line Flush Section 6 5 before installing potassium perma...

Страница 29: ...roup formulation instructions NOTE Immediately remove spills in the reagent tray to preserve the accuracy of the reagent level detecting system 3 For all reagents using the standard 500 mL bottles Ope...

Страница 30: ...all the reagent detection cable from the 5 L bottle cap to the connector on the back of the instrument 6 Refer to Using Reagent Information Tracking in Section 3 1 for more information on tracking rea...

Страница 31: ...the motor shaft CAUTION Fluid from priming can flood and damage the motor if the drain tube is not properly installed 4 Press Maintenance from the Main menu 5 Press the A prime button Stain should ap...

Страница 32: ...ime all lines simultaneously To prime individual lines press the appropriate individual prime button A B C D E The pumps will run for 1 minute and prime the selected lines Run at least 200 mL of reage...

Страница 33: ...ts sprayed from the nozzles when you press Clean or Standby 2 Press Clean NOTE Pressing Stop during the Clean cycle causes the Incomplete Clean message to be displayed Press Clean to complete the inte...

Страница 34: ...y line not using the standard 500 mL bottle except Line D Line D features a level detection option for the 5 L external bottle CAUTION This system is designed to warn you when the reagent level is get...

Страница 35: ...level detect is not reporting correctly If zeroing does not correct the problem recalibrate the Level Detect function Section 7 3 1 Press Information 2 Press System Setup 3 Press Level Detect to ente...

Страница 36: ...ibrations or bumps to the instrument or lab bench can cause inaccuracies in zeroing or calibration 6 After zeroing press OK Press the Back button to exit to the System Setup menu 7 Return the reagent...

Страница 37: ...s Managing reagent and waste level sensing Changing the display language Viewing and exporting the system log Changing beeper settings Setting the date and time Restoring default settings Accessing th...

Страница 38: ...ng the sliding tabs 5 Press Save Adjusting Stain Settings 1 From Stain Settings menu select the Primary Decolorizer or Counterstain Dry tab 2 Select and adjust the desired stain parameter using the sl...

Страница 39: ...istrator account at least 4 characters 4 Re enter the password to confirm Creating User Accounts 1 Select System Setup 2 Enter the Administrator password 3 Press Users 4 Select Enable Global Login 5 S...

Страница 40: ...Logout After Idle drop down menu 2 Press Login 3 Enter the correct passcode for the selected user and press Enter 4 The stainer returns to the Main menu and is ready for programming and staining 5 Onc...

Страница 41: ...ts to ON for reagent monitoring and to OFF for waste container monitoring See Section 2 2 for complete instructions 1 From System Setup select Level Detect 2 Select the reagent monitoring options to b...

Страница 42: ...Use navigation arrows to scroll through the log Exporting Logs 1 From System Setup Press System Log 2 Plug a Flash Drive into the right USB port 3 Press Export NOTE The log files are exported to the...

Страница 43: ...cking NOTE Selecting Enable Stain Slide Tracking changes the Start button on the Main menu to Load Slides 3 Press Back twice to return to the main screen Verify that the Start Button on the main scree...

Страница 44: ...ve Maintenance Tracking To activate the tracking prompts for Preventive Maintenance Tracking use the Following steps 1 From System Setup select QC Maintenance Tracking 2 Select Enable Preventive Maint...

Страница 45: ...lete all user names and passwords as well as all stain and cytocentrifuge programs Restoring Stain Settings will delete all stain programs and restore the default program Restoring Cytocentrifuge Sett...

Страница 46: ...s the Start button on the Main menu to Load Slides See Scanning Slides with the Barcode Reader Section 3 2 3 Press Back twice to return to the Main menu 4 Press Load Slides on the Main menu The Scan a...

Страница 47: ...elect QC Maintenance Tracking 2 Select Enable Reagent Tracking for each desired reagent A B C D E 3 Press Back to return to the System Setup menu 4 Press Reagents to access the Reagent Information scr...

Страница 48: ...the Main menu 2 Press Add to access the keypad 3 Enter slide information maximum of 24 characters and press Enter 4 To change or delete the entry select the entry on the display and press Edit or Rem...

Страница 49: ...elp Setting Up Cyto Programs Setting Up Users Setting Up Level Monitoring System Setting Instrument Language Setting the Date and Time Instrument Logging Setting Up Network Settings Setting Instrument...

Страница 50: ...Select or verify desired stain program Perform a stain cycle Unload the carousel Performing a Hub Pattern Test Use the Hub Pattern Test to ensure the nozzles are clear of debris and spraying properly...

Страница 51: ...r a similar level of staining There is no guarantee of staining performance when dissimilar specimens are used 1 Remove the carousel from the bowl and place it on a solid level surface 2 Remove the ca...

Страница 52: ...ide in position 1 the second in position 2 and so on NOTE A warning will sound during the staining cycle if the carousel is unbalanced Figure 9 Loading a 12 Slide Carousel Slide Labels Toward Outer Ri...

Страница 53: ...mbinations of reagents to be loaded on the stainer Appendix D in combination with programming adjustments Section 3 1 Insert a carousel with specimen slides and close the instrument lid 2 If you have...

Страница 54: ...progress of the program and a signal tone if enabled indicates the end of the cycle NOTE Use the emergency Stop button when required for example if there is abnormal vibration or noise This will abort...

Страница 55: ...mpared to the actual level in the bottles Never allow a reagent to run dry When the reagent level is near empty replace the reagent bottle with a new one see below Never allow the waste container leve...

Страница 56: ...de of the Main menu 3 If you are using reagent tracking scan the barcode or manually enter the reagent REF expiration date and lot number in the Using Reagent Information Tracking menu Section 3 1 4 O...

Страница 57: ...is functioning as required for acid fast staining in a laboratory environment 5 At the end of the day Place an empty carousel in the bowl and close the lid Press the Standby Ready button on the front...

Страница 58: ...ance Log With Preventive Maintenance Tracking enabled the PM Log provides a convenient and structured means of recording important maintenance and QC functions The system allows you to set up timely p...

Страница 59: ...the dip tube in a bottle of approved alcohol 4 Flush at least 250 mL of approved alcohol through the reagent line by priming all lines simultaneously Leave the alcohol in the line NOTE When using pot...

Страница 60: ...wer OFF the instrument 2 Disconnect the power cord from the power outlet and the rear panel of the instrument 3 Open the fuse cover by inserting a screwdriver in the slot on the right side of the cove...

Страница 61: ...ge to the equipment WARNING If potentially infectious liquid is spilled on the instrument the instrument must be disinfected in accordance with all applicable local regulations Refer to Decontaminatin...

Страница 62: ...uding gloves and eye protection when handling reagents WARNING Authorized trained personnel wearing appropriate protection equipment should perform the decontamination procedure in a well ventilated r...

Страница 63: ...eaning solutions to dry on the instrument surfaces 6 Rinse the inner bowl and lid thoroughly with water 7 Spray and wipe the exterior surfaces with decontamination solution such as REF SS 133 CAUTION...

Страница 64: ...deionized or distilled water bottle with a fresh 24 hours old 10 dilution of household bleach solution 5 25 sodium hypochlorite 2 Allow the bleach solution to react in the bottle for 10 minutes 3 Rin...

Страница 65: ...ontamination performed by Elitech Group CAUTION Ship the instrument in a container comparable to its original packaging Disinfection Declaration The operating authority must print and complete the dis...

Страница 66: ...be easily loosened use light penetrating oil and a 5 8 in wrench to loosen the nozzle 3 Place the nozzle parts in a 50 mL conical tube that has been clearly marked with the correct nozzle position 4...

Страница 67: ...rew threads 7 Reassemble the nozzle by placing the compression screw on the nozzle disassembly tool then inserting the swirl cone into the compression screw NOTE Hold all the parts in a vertical posit...

Страница 68: ...tube until the fluid is free of bubbles then press Stop WARNING Do not pull the plunger completely out of the priming tool Pulling the plunger out of the tool may result in splashing or spraying of r...

Страница 69: ...he following table Table 10 Volume Test Tolerances Carbol Fuchsin Stains Nozzle Reagent Line Minimum Maximum A B 8 5 mL 11 0 mL C 8 5 mL 13 0 mL DF DR 8 0 mL 10 5 mL E 9 0 mL 12 0 mL Table 11 Volume T...

Страница 70: ...m persists replace the nozzle NOTE If the problem persists after you have replaced the nozzle contact Elitech Group 9 Prepare the Maintenance Kit for future use Empty the contents of the tubes into th...

Страница 71: ...emove the B or C nozzle or both depending on the desired line flush 2 From the Maintenance menu press Line Flush 3 Select the desired line or lines to be flushed 4 Load 500 mL of deionized or distille...

Страница 72: ...400 mL of water through the line s A status bar will indicate progress 13 When the flush is complete remove the remaining water 14 Load at least 300 mL of the correct reagent for that position into th...

Страница 73: ...locking slide in front of positions 1 and 2 2 Load the carousel into the stainer and close the lid 3 From the Main menu select Maintenance 4 Press Pattern Test 5 Press the corresponding prime button f...

Страница 74: ...frigerators air conditioners or other motorized equipment If the above steps do not solve the problem consult the Aerospray Service Manual or contact your dealer or Elitech Group for assistance A reag...

Страница 75: ...the slide carousel is properly loaded on the drive hub In staining mode the instrument detects whether the staining carousel is present before proceeding In cytocentrifuge mode the instrument will sto...

Страница 76: ...dealer or Elitech Group for assistance If the cycle continues this indicates an electronic problem see below Abnormal staining on entire surface of all slides Check the reagent level on the display a...

Страница 77: ...s are still attached to the bottom of the carousel Make certain you have not programmed the stainer for fewer slides than you have loaded If you program the stainer for a partial load load the slides...

Страница 78: ...lose its place 1 If this occurs switch the main power OFF for 10 20 seconds and then back ON to reset the instrument 2 If the problem recurs install a computer type surge protector to isolate the ins...

Страница 79: ...result in a partially acid fast positive appearance in the smear s AFB negative bacteria and background due to incomplete removal of the primary stain Possible causes for under decolorization include...

Страница 80: ...her 100 mL of disinfectant solution through each affected reagent line and let sit for 20 minutes 3 Flush each affected reagent line with 150 mL of deionized water 4 Reprime the stainer with fresh rea...

Страница 81: ...e been verified the instrument may require service A reagent line leak between the pump outlet and the nozzle will cause fluid to leak into the interior of the stainer housing and ultimately onto the...

Страница 82: ...shows an error message Calibrate again making sure that the correct reagent bottles are placed in the enabled tray positions LD Level Detection Unstable If bottle movement is detected while calibratin...

Страница 83: ...Detect System is reporting incorrectly and zeroing Section 2 2 does not correct the problem calibrate the system as follows 1 Press System Information from the Main menu 2 Press System Setup 3 Press...

Страница 84: ...ent placed in the correct tray positions due to different densities of each reagent type 6 Place the correct reagent bottles in all enabled positions and press Start The display shows NOTE The calibra...

Страница 85: ...appears 2 Select the center of the target with a finger stylus or similar tool Another target will appear in a different location 3 Continue to press the center of the targets until you have pressed...

Страница 86: ...offer full local service and support ELITechGroup Inc 370 West 1700 South Logan Utah 84321 8212 USA Telephone 800 453 2725 United States Canada 1 435 752 6011 International calls Fax 1 435 752 4127 Em...

Страница 87: ...cations for settings speed acceleration rate and time Easy switching between staining and cytocentrifuge modes Autoclavable rotor NOTE Pressing Cyto brings up the Cytocentrifuge mode Pressing Back ret...

Страница 88: ...s 1 Methylene Blue 1 Benzalkonium Chloride 10 Ethanol SS 061BBG or SS 061BBG EU Aerospray TB Reagent B Brilliant Green SS 161 BBG Aerospray TB Reagent B Brilliant Green Concentrate when diluted proper...

Страница 89: ...anol 5 Brilliant Green 2 Malic Acid 1 Benzalkonium chloride SS 161BP Aerospray TB Reagent Potassium Permanganate Concentrate contains 10 Potassium Permanganate SS 161M Aerospray TB Mycohold Cell Adhes...

Страница 90: ...the following Risk and Safety Phrases The associated EU Symbol of Danger is none R 10 Flammable S None Reagents SS 061BBG and SS 061BBG EU Aerospray TB Reagent B Brilliant Green and SS 161BBG Aerospra...

Страница 91: ...24 25 Avoid contact with skin and eyes S 26 In case of contact with eyes rinse immediately with plenty of water and seek medical advice S 28 After contact with skin wash immediately with plenty of wat...

Страница 92: ...ive Concentrate is associated with the following Risk and Safety Phrases The associated EU Symbol of Danger is Xn R 22 Harmful if swallowed R 52 53 Harmful to aquatic organisms may cause long term adv...

Страница 93: ...erse effects in the aquatic environment S 61 Avoid release to the environment Refer to special instructions safety data sheet SS 266 and SS 266 EU PM Cleaning Solution is associated with the following...

Страница 94: ...possible SS 029 Aerospray Nozzle Cleaning Solution and SS 029C when diluted as instructed are associated with the following Risk and Safety Phrases European Symbols of Danger are F and T R 11 Highly...

Страница 95: ...L Bottle Assembly with level detect AC 183 Nozzle Maintenance Kit AC 184 2D Barcode Scanner AC 185 SUPPLIES Carbol Fuchsin Ready To Use Reagents Carbol Fuchsin Decolorizer Reagent 500 mL SS 061A or SS...

Страница 96: ...1 0 L SS 161M Miscellaneous Supplies Nozzle Cleaning Solution 355 mL SS 029 Nozzle Cleaning Solution 250 mL dilutes to 500 mL Concentrate SS 029C or SS 029C EU Nozzle Cleaning Solution 1 89 liter dil...

Страница 97: ...he 12 place carousel or 30 place carousel The stainer automatically adjusts programming for the carousel that is loaded in the stainer Users should adjust the programming as necessary depending on whi...

Страница 98: ...about reagents Methylene Blue Ready to use SS 061BMB or SS 061BMB EU Concentrate SS 161BMB Brilliant Green Ready to use SS 061BBG or SS 061BBG EU Concentrate SS 161BBG NOTE Brilliant green and methyl...

Страница 99: ...No concentrate available NOTE Auramine O and Auramine Rhodamine are interchangeable according to customer preferences D Reagent Deionized Water E Reagent Approved Alcohol Methanol SS MEOH available i...

Страница 100: ...mall enough that the slides are completely dry less than 1 minute after being removed from the stainer about the length of time to take the slide to the microscope Most users will use the default sett...

Страница 101: ...usel mL 0 4 4 5 5 5 5 7 8 5 10 11 5 13 14 5 16 17 5 19 20 5 22 Counterstain Wash Setting 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 Counterstain Wash total number of applications per slide 0 2 4 7 10 12 14...

Страница 102: ...20 22 24 26 28 30 32 Approximate water D line usage for a full carousel mL 0 12 24 42 60 72 84 96 108 120 132 144 156 163 175 187 Counterstain Setting 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 Countersta...

Страница 103: ...a NALC 4 NaOH preparation For each day s cultures add up the total volume of specimens to be treated and prepare an equal volume of digestant decontamination mixture as follows 1 N 4 NaOH 50 mL 0 1 M...

Страница 104: ...ximum exposure of 20 minutes 6 After 15 minutes of digestion add enough phosphate buffer to reach within 1 cm of the top of the tube screw the cap tightly closed and invert the tube to mix the solutio...

Страница 105: ...not been tested for viability of organisms after fixation 3 For thick specimens or for users that experience frequent sample loss during staining Elitech Group recommends using Mycohold Mycohold was d...

Страница 106: ...lways handle all specimens with proper precautions Staining Slides 1 After sample fixation the slides are ready for staining with the Aerospray TB stainer Stain slides using either carbol fuchsin or f...

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