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18

Fig 31

3.3.2

Push the blank cuvette into the Reference Light Path

and Main Light Path, press

【

0Abs/%100T

】

,the

instrument will step to the wavelength and blank. See

Fig 32.

Fig 32

3.3.3

Pull the first sample cuvette of known concentration into

the light path, Press the key

【

START

】

to get values of

standard curve one by one (Fig 33).

3.3.4

Press

【

F4

】

to draw the curve. You can get a different

curve by pressing

【

F1

】

to select a different fitting method.

(See Fig 34-Fig37.) For linear fits, “ r ” represent fitting

coefficient of linear regression .r=1 is best fitting.usually

“ r ” is very close to 1.

Note:If there are few standard samples, it is not suitable for

selecting square fitting, especially cubic fitting, otherwise invalid

fitting result will be obtained.

Fig 33

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Содержание DU-8800D Series

Страница 1: ...USER S MANUAL For DU 8800D Series Spectrophotometers Drawell Scientific...

Страница 2: ......

Страница 3: ...Operation 5 Prepare the Spectrophotometer 5 Description of keys 6 Turn on spectrophotometer 7 Basic operation 8 Analyse Sample 13 Basic Mode 13 Quantitative 14 WL Scan 20 Kinetics 26 DNA Protein 29 Mu...

Страница 4: ......

Страница 5: ...tometry are corrosive and or inflammable and samples may be radioactive toxic or potentially infective Care should be taken to follow the normal laboratory procedures for handling chemicals and sample...

Страница 6: ...are used when connecting the instrument to computers and accessories Introduction This instrument Fig 1 is a double beam general purpose instrument designed to meet the needs of the Conventional Labor...

Страница 7: ...rt it to an electrical signal and 5 A digital display to indicate absorbance or transmittance The block diagram Fig 2 below illustrates the relationship between these parts Block diagram for the Spect...

Страница 8: ...ophotometer 1 Mains Lead 1 Glass Cuvettes 1 Set of 4 Quartz Cuvettes 1 Set of 2 Dust Cover 1 Operation Manual 1 Software Manual 1 Software Kit Disc 1 USB Lead 1 Dongle 1 1 Fuse 2 Note The printer and...

Страница 9: ...als that could hinder the flow of air under and around the instrument 4 Use the appropriate power cord and plug into a grounded outlet 5 Turn on your spectrophotometer Allow it to warm up for 15 minut...

Страница 10: ...he inputted data or selected item Go into next setup or screen F1 F4 Function based on the information on the screen 0 9 Input number or letter consecutively press a numeric key to select a character...

Страница 11: ...positioning filter auto cell changer if installed and D2 W lamps the screen display as Fig 4A 15 minutes pass or press ESC the screen display as Fig 5 Select No to skip to main menu Fig 7 and select Y...

Страница 12: ...rebuild the system baseline Select Yes in Fig 5 or Press 0 In Fig 73 or Press F4 In Fig41 Regarding Blanking important points list below A Take measure in Basic Mode a Put the reference cuvette with...

Страница 13: ...etc B Take measure in WL Scan a After all scan parameters are entered put the reference cuvette with reference solution into the Reference Light Path and the sample cuvette with sample solution into...

Страница 14: ...ing pressed the first file ABC wav in memory will appear on the bottom line of screen Showed as Fig 13 Press or to browse the files stroed in memory Then if 1 The key ENTER be pressed the file selecte...

Страница 15: ...Press the key SAVE in Fig14 to save curve Name the curve by pressing the numeric keypad Fig 15 press the key ENTER to confirm Note 1 Pressing numeric key continually to scroll characters and pressing...

Страница 16: ...xample Print the report in Basic mode Fig16 Press the key PRINT to print the report curve or data you have loaded or tested Fig 17 Fig 16 Fig 17 Before measurement Make a blank reference solution by f...

Страница 17: ...ress 1 to enter Basic mode test After automatically blanking it will display as Fig 18 automatic changer installed or Fig 19 automatic changer uninstalled and wait for the operator ESC STOP to exit No...

Страница 18: ...eric keypad Fig 22 Fig 21 Fig 22 4 Push the blank cuvette into the Reference Light Path and Main Light Path and press ZERO for Blanking There are now two choices for you to take 4 1 Press F3 to input...

Страница 19: ...Test Report Press PRINT to print test results Fig 25 Fig 25 Quantitative Press 2 in Main Menu for Quantitative Test Fig 26 Press ESC STOP to exit Note If no automatic changer installed cell 1 will dis...

Страница 20: ...are three correction methods single Isoabsorbance and 3 point Fig 28 Note Please refer to the Appendix B for the correction method Fig 28 3 Press F2 in Fig 26 for more items to select See Fig 29 Fig 2...

Страница 21: ...A K1 r Linear fit C K0 K1 A K0 K1 r square fit C K0 K1 A K2 A2 K0 K1 K2 cubic fit C K0 K1 A K2 A2 K3 A3 K0 K1 K2 K3 r regression co efficients default 1 3 3Press F3 in Fig 29 to establish a standard c...

Страница 22: ...RT to get values of standard curve one by one Fig 33 3 3 4 Press F4 to draw the curve You can get a different curve by pressing F1 to select a different fitting method See Fig 34 Fig37 For linear fits...

Страница 23: ...19 Fig 34 linear through zero fit Fig 35 square fit Fig 36 cubic fit Fig 37 linear fit 3 3 5 Press SAVE to save calibration if required 3 3 6 Press ESC STOP to exit...

Страница 24: ...the standard samples for the test First the standard curve must be established using the method shown in 3 3 Note All sample results must be taken in screen Fig26 4 1 Push the blank cuvette into the...

Страница 25: ...length by pressing the numeric keypad Fig 42 Note This instrument scans from high to low wavelength Browse and select the items of scan step and scan speed by pressing or Fig 42 Note Scan step allows...

Страница 26: ...n Light Path press ZERO to scan the base line Fig 44 Press the key ESC STOP to stop scanning Fig 44 4 Put the sample cuvette into Main Light Path press START to scan the sample Fig 45 ESC STOP to stop...

Страница 27: ...e Fig 47 input upper limit and lower limit by pressing the numeric keypad To change y scale press or After these inputs the instrument will redraw the curve Fig 48 Fig47 Fig 48 6 Press F3 to search th...

Страница 28: ...splayed on the screen one at a time Fig 51 Fig 50 Fig 51 2 Point to point Press to search the point from left to right and press to search from right to left The search step interval is the same as th...

Страница 29: ...curve you have loaded or scanned Fig 52 Note The report always is printed in Fig 46 Fig 52 Kinetics Press 4 in main menu for Kinetics Fig 53 ESC STOP to exit To load a previous kinetics result press L...

Страница 30: ...y pressing F2 Fig 55 Fig 55 3 Set wavelength by pressing SET Pull the blank cuvette into the Reference Light Path and Main Light Path press ZERO for blanking 4 Pull the sample cuvette into Main Light...

Страница 31: ...line between the Begin Time and End Time will be calculated The gradient of this line gives the rate of change of A min Note I U Factor A min Fig 57 Fig 58 6 If you want to change the scale please re...

Страница 32: ...E to save curve Note Load Save requires the first kinetics display page Fig 56 Press ESC if in Search to return to the required page Print Test Report Press the key PRINT to print the curve you have l...

Страница 33: ...nt algorithm you can enter your own values for f1 f4 Press F1 to set f1 f4 Input the value by pressing the numeric keypad Fig 61 Fig 61 2 Press F2 to select test mode Absorbance difference 1 is for te...

Страница 34: ...g 5 Pull the sample cuvette into Main Light Path press START to test the sample The test result will be displayed on the screen Fig 65 Fig 65 6 If there is more than one sample repeat step 5 for the n...

Страница 35: ...ta Press the key SAVE to save data Print Test Report Press the key PRINT to print the test result Fig 67 Fig 67 Multi Wavelength Press 6 in main menu for Multi WL Fig 68 ESC STOP to exit Fig 68 To loa...

Страница 36: ...OP to finish setup and exit Note It is recommended to enter the highest wavelength first Fig 69 2 Press F2 to select mode Fig 70 Fig70 3 Push the blank cuvette into the Reference Light Path and Main L...

Страница 37: ...t has finished the wavelength will go to the first WL 6 Press or for searching Input the sample number the result will be displayed on the screen Press or to browse the test results one by one Save Da...

Страница 38: ...tting and Calibration Utility Press 7 in Main menu for Utility Fig 73 ESC STOP to exit Fig 73 WL Reset Press 1 to reset wavelength Fig74 Fig 74 Printer Press 2 to set printer Fig 75 ESC STOP to exit F...

Страница 39: ...ig 75 to select printer HP PCL 1 colour cartridge PCL black mode Epson ESC P or Epson P2 or above Fig77 Fig 77 4 Press 4 in Fig 75 to select print mode If you select Print screen mode a little icon wi...

Страница 40: ...s 1 in Fig 79 to switch on off D2 Fig 80 Fig 80 2 Press 2 in Fig 79 to reset usage time of D2 Fig 81 Press or to select Yes or No and then press ENTER Fig 81 3 Press 3 in Fig 79 to switch on off W The...

Страница 41: ...age of W Fig 83 Press or to select Yes or No and then press ENTER Fig 83 5 Press 5 in Fig 79 to set the switch usage point of D2 and W lamp Fig 84 84 Clock Press 4 In Fig73 to set the display mode and...

Страница 42: ...c keypad Fig 86 Fig 86 2 Press 2 in Fig 85 to modify date by pressing the numeric keypad 3 Press 3 in Fig 85 to set the date display on the top right corner of the screen 4 Press 4 in Fig 85 to set th...

Страница 43: ...ESC STOP to exit Fig 89 1 Press SET to set the wavelength Press ENTER to edit and input wavelength by pressing the numeric keypad Fig 90 ESC STOP to finish inputting and exit Fig 90 2 Press F1 to set...

Страница 44: ...sample calibrated neutral density filter into Main Light Path Press START to check The results will be displayed on the screen Fig 94 If the discrepancy between the results and the calibrated standar...

Страница 45: ...validity Fig 95 ESC STOP to exit Fig 95 2 Press F1 to set the standard peak Press ENTER to edit and input wavelength by pressing the numeric keypad Fig96 ESC STOP to finish inputting and exit Fig 96 3...

Страница 46: ...check The results will be displayed on the screen Fig 99 If the discrepancy between the results and the calibrated values is not more than the tolerance pass will be displayed after the test results O...

Страница 47: ...100 Fig 100A Beeper on off Press 9 in Fig 73 to turn on off the beeper Delete entire saved files Press F1 in Fig 73 to delete entire saved files After the delete the files double confirm need to do R...

Страница 48: ...cover Please wipe off the dirt on the cover timely 3 Clean the cuvettes After every test or after a solution change the cuvettes should be cleaned carefully or the remains on the surface would cause...

Страница 49: ...pare Fuse and replace it to the working position Step 5 Reset the Fuse Seat Replace the Fuse Seat in the power socket Step 6 Switch on the power Plug the socket and switch on the power 2 Replace lamps...

Страница 50: ...crews on the D2 Flange Indicated in the Red Circles in Fig 105 unplug the power connector Indicated in the Red Square in Fig 104 in the Power Board and remove the D2 lamp Draw on the Cotton Glove and...

Страница 51: ...me direction as the old one faces Fig 106 Fig 106 Switch on the power observe the Entrance Facula and it should focus on the center of the Slit Fig 107 If the Facula deviate to Left or Right then loos...

Страница 52: ...48 Fig 107 Step 8 Finish Reset the cover of the Lamps chamber and fix the screws Reset the cover of the instrument and fix the screws then the course finished...

Страница 53: ...l DNA concentration A1 Aref f1 A2 Aref f2 Protein concentration A2 Aref f3 A1 Aref f4 f1 62 9 f2 36 0 f3 1552 f4 757 3 DNA g ml Protein g ml Absorbance difference 260 230 A1 A260nm A2 A230nm Aref A320...

Страница 54: ...oabsorbance When a known interfering component with a known spectrum is present the error introduced by this component at the analytical wavelength for the target analyte can be eliminated by selectin...

Страница 55: ...en estimated using linear interpolation see Figure A2 This method represents an improvement over the single wavelength reference technique because it corrects for any background absorbance that exhibi...

Страница 56: ...52 Drawell International Technology Limited Shanghai Drawell Scientific Instrument Co Ltd Add Suite 1506 Lane581 XiuChuan Rd PuDong New Area Shanghai China Tel 0086 21 54411195 Fax 0086 21 33823261...

Страница 57: ...53 Web www drawell com cn Email sales01 drawell com cn...

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