8
9. Mount the gas-tight syringe onto the motorized syringe driver as follows:
a. Slacken the flange clamp – labeled below
b. Feed the plunger back into the syringe barrel
c. Place the barrel into the V on the top of the syringe driver nose,
ensuring that the
flange of the syringe barrel is between the nose and the flange clamp
d. Tighten the barrel clamp
e. Tighten the flange clamp. The flange clamp and the barrel clamp should be done
up finger-tight only.
10. Turn the upper valve to the flush position (
├
), and the lower valve to the dispense
position (
┬
). Place a waste beaker under the lower valve. Flush air and bubbles out of
the connecting tubing between the upper and lower valves.
11. Repeat steps 1-8 for the remaining low volume channels.
Installing 2.5 ml syringe
Undo the two clamps on the last syringe driver and install the 2.5ml syringe. Do up the two
thumbscrews tightly. Connect the corresponding end-fitting to the last valve.
Flange clamp - ensure
that it is under the syringe
Do up barrel
clamp finger-tight
Do up flange
clamp finger-tight