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Douglas Oryx 4, Руководство по оборудованию

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25

Index of Manual 

 

Adjustment of Plate Loader, 20 
Blockages, 18 
Bubbles, removing, 15 
Chemical inertness, 18 
Cleaning microtip, 18 
Debubbling, 15 
Degassed water, use of, 15 
Electrical connection, 5 
Filling Microtip with solution, 16 
Flushing Microtip after use, 18 
Flushing tubing between valves, 

16 

HARDWARE.CFG, 22 
HARDWARE.FTH, 22 
Installing Microtips, 15 
Installing the Z and V arms, 5 
Lay-out of the hardware, 4 
Logging motor positions to disk, 

5 

Lubrication, 20 
MCC Motion Control Center, 4 
Microtip 

collisions with Plate 

Loader, 15 

installing, 15 
protein coatings, 18 
unblocking, 18 

Perfomance of Plate Loader - 

testing, 9 

Plate Loader 

tesing perfomance, 9 

Plates 

adding, 21 
changing, 21 
changing, 21 

Plates, adding new designs, 21 
Recording the positions of 

motors, 5 

Refilling Motorized Syringes with 

water, 15 

Rezeroing motorized syringes, 6 
Rezeroing Plate Loader, 10 
Software installation, 4 
Specifications, 24 
Spillages, 20 
Syringes 

debubbling, 15 
filling with degassed 

water, 6 

mounting, 6 

Syringes, changing, 21 
Tesing perfomance of Plate 

Loader, 9 

Tubing connections, 18 
Unblocking Microtips, 18 

 
 
 
 

Содержание Oryx 4

Страница 1: ...guration Revision 5 3 August 2006 Compiled by Patrick Shaw Stewart Peter Baldock and James Smith Copyright 1991 2006 Douglas Instruments Ltd Douglas Instruments Limited Douglas House East Garston Hung...

Страница 2: ...ility to observe any special handling procedures The Red stop button on the MCC will terminate any movement of the plateloader arms and syringes The purpose being to avoid spillage or other loss of oi...

Страница 3: ...ringes with Water 15 Debubbling 15 Flushing Tubing between Valves 16 Filling Microtip with Solution 16 Dispensing Experiments 16 Attach the Evaporation shield for Vapor Diffusion Experiments 17 Flushi...

Страница 4: ...are Layout of the Hardware The layout of Oryx4 and Oryx8 is shown in the figure below The system is driven by a PC which is connected to the MCC Motion Control Center The motorized syringes and the XY...

Страница 5: ...ateloader out of their packaging Unwrap the Z and V arms Take the motorized syringes and ground glass syringes out of their packaging Electrical Connection 1 All cables are individually labeled Use th...

Страница 6: ...of their travel the syringe drives will miss steps and make a rattling noise This will not harm the syringe drivers in any way When you are sure that all of the syringe drives have reached their lowe...

Страница 7: ...8 Place the barrel over a waste container and flush all air and bubbles out of the connecting tubing and gas tight syringe To ensure that no bubbles are left in the upper valve tap the plunger of the...

Страница 8: ...uld be done up finger tight only 10 Turn the upper valve to the flush position and the lower valve to the dispense position Place a waste beaker under the lower valve Flush air and bubbles out of the...

Страница 9: ...Clear Tip Offsets this clears any adjustment made to the tip position 3 Install the mounted needle into the Z arm you should find this in the red plastic box 4 Click Plate Loader Diagnostics Align Tab...

Страница 10: ...e centre of the collet before starting an experiment Rezeroing all Motors of the Plate Loader 1 Switch on PC and MCC and start the program Front Panel as described above 2 Actions Rezero Follow instru...

Страница 11: ...e Experiment Resume Current Experiment Abort Experiment Abort Current Experiment Exit Exit Front Panel Actions Menu All of these items can also be found in other menus but are grouped here for conveni...

Страница 12: ...ecalibrate start positions use in case syringes or Plateloader was obstructed during use Power Down all motors Turns off power to all motors to allow manual positioning of the table or to stop the noi...

Страница 13: ...sition and the Power Down Timer starts Rezero Table Rezeros the Plateloader table Solutions Menu Debubble Run the Debubble wizard to remove air from the tubing above the top valves Protein Load Protei...

Страница 14: ...ed for diagnostics Execute in Background Allows Front Panel to execute in the background while using other applications Default Off Advanced Menu Items On Off Enables Menu Items for advanced users Def...

Страница 15: ...s with Water The 100ml syringes contain only degassed pure water This means that there is no need to flush them when the stock solutions are changed Degassing is very helpful in reducing bubbles When...

Страница 16: ...o the disposable syringe This prevents debris from being flushed out of the microtip which could cause blockages Filling Microtip with Solution Software will tell you when to fill the Microtip with st...

Страница 17: ...e a gap for the shield 2 Place the shield onto the plate with the grove on the side sliding over the screw in the slider 3 Put light pressure on the shield above the plate with one hand and tighten th...

Страница 18: ...s previously contained protein unless it has been cleaned using alkali or acid see above Filter all solutions using a 0 45 mm filter or equivalent and refilter any solutions that become cloudy or cont...

Страница 19: ...itting 3 Grip the end of the tube with a pair of pliers and pull the tube through the gripper fitting beyond the tapered portion of the tube onto its full diameter and rotate twice to grip the tube 4...

Страница 20: ...lubricated or if they become corroded Spillages If large amounts of liquids are spilt on any of the electric motors the system should be turned off Salt acid or alkali will cause corrosion of motors a...

Страница 21: ...y a matter of using a text editor to modify the existing file The PLATE declaration statements are always near the top of the XPT file and refer to plates by type name e g Nunc HLA refers to a standar...

Страница 22: ...0 steps mm Speed 4 00 mm s Range 75 00 mm Length 92 00 mm Drive 5 Channel 5 Pitch 314 9600 steps mm Speed 4 00 mm s Range 75 00 mm Length 92 00 mm Drive 6 Channel 6 Pitch 314 9600 steps mm Speed 4 00...

Страница 23: ...der pitches and speeds 9 0 533 setCurrent 9 321 0 setLength 10 0 533 setCurrent 10 151 0 setLength 11 0 400 setCurrent 11 50 0 setLength 12 0 400 setCurrent 12 52 0 setLength 9 13 3477 setPitch 9 70 0...

Страница 24: ...24 Specifications An up to date list of specifications for all Douglas Instruments products can be found at http douglas co uk specs htm...

Страница 25: ...5 Lubrication 20 MCC Motion Control Center 4 Microtip collisions with Plate Loader 15 installing 15 protein coatings 18 unblocking 18 Perfomance of Plate Loader testing 9 Plate Loader tesing perfomanc...

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