10
10. Click
Options
|
Administrator Mode
– to turn off Administrator mode
11. Click
Options
|
Advanced Menu Items
- to turn off Advanced Menu Items
Please note:
When you align the tip on the destination plate in an experiment these offsets are used on all
other plates. If the tip is bent and the end of the tip is far away from the centre of the collet
then it may appear to be off centre when it loads protein. If you look closely you should see
that the end of the tip is in the centre of the protein tube when it starts to go down, but the
collet may be so far off centre that it hits the side of the tube.
It is best to try to straighten the tip by hand or to adjust the tip in the guide loop to be pointing
straight down and in the centre of the collet before starting an experiment.
Rezeroing all Motors of the Plate Loader
1. Switch on PC and MCC, and start the program
Front Panel
as described above.
2.
Actions | Rezero
. Follow instructions to rezero Syringe Drives, Z, V, X and Y motors.
The Z and V motors will make a loud rattling noise when they are rezeroed. This noise is caused
by the motors missing steps. This will not harm the system in any way. When the loud noise
starts it can be cut short by pressing the red Stop Button (or by clicking on the red Stop Button on
the screen).
If this rezeroing routine is not correctly carried out, mechanical errors may occur (e.g. the microtip
may be damaged, and the gas-tight syringes may be broken). However, it need only be carried
out during the initial setup, or if there seems to be an error with the motor positions. Normally the
motor positions are saved to disk after use of the system, and rezeroing is not necessary.