31
a)
b)
Fig.22. Check of geometrical settings (a – M modification, b – X modification)
4.2.2. Optical Measurement Exposure Setting
Preset working exposure values for each channel are the following:
Fam – 1000; Hex – 1000; Rox – 1000; Cy5 – 1000; Cy5.5 – 1000
Note!
Preset parameters of optical measurements for each channel are optimal to operate with
DNA-Technology PCR kits and could be suitable for the most of third party kits.
Exposure change is required only when levels of different channels’ optical signals
substantially differ, as it may result in incorrect color compensation in the process of analysis
of optical measurements. For exposure setting window see Fig. 23.
Caution!
In case of using third party kits the optimal exposition values for channels must be
provided by manufacturer.