DMT 750 TOBS, Руководство пользователя

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14
APPENDIX 1 - BUFFER RECIPES
PHYSIOLOGICAL SALINE SOLUTION (PSS)
1x PSS (1000ml):
Solution 1:
Chemical
MW (g/mol) Conc. (mmol/l) Conc. (g/l)
NaCl 58.44 118.99 6.954
KCl 74.56 4.69 0.350
MgSO
4
-
7
H
2
O 246.48 1.17 0.289
KH2PO4 136.09 1.18 0.161
Solution 2:
Chemical
MW (g/mol) Conc. (mmol/l) Conc. (g/l)
CaCl
2
-
2
H
2
O 147.02 2.50 0.368
Solution 3:
Chemical
MW (g/mol) Conc. (mmol/l) Conc. (g/l)
NaHCO
3
84.01 25.00 2.100
EDTA 372.24 0.03 0.010
Glucose 198.77 5.50 1.091
1. Dissolve the chemicals in 100 ml double distilled H
2
O as three individual solutions as described in the
table above. Gently heat solution 3 to dissolve the EDTA.
2. Solution 1 is added to a graduated bottle and the bottle is filled with double distilled H
2
O to a final
volume of 500 ml.
3. Solution 3 is added to the graduated bottle, which afterwards is filled with additional double distilled
H
2
O to a final volume of 850 ml.
4. Aerate the solution with carbogen (95% O
2
+ 5% CO
2
) for about 20 minutes.
5. Solution 2 is added and the graduated bottle is filled with additional double distilled H
2
O to reach the
final volume of 1000 ml. Continue the carbogen bubbling until the pH of the buffer solution reaches
4.4.