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CHAPTER 3: CLEANING AND MAINTENANCE
3.1 CLEANING THE TISSUE ORGAN BATH SYSTEM
The 750TOBS is a very delicate and sophisticated piece of research equipment. In order to keep it working
at its best, DMT recommend that the following sections are read carefully and that the instructions are
followed at all times. DMT strongly recommends that the Tissue Organ Bath and surroundings should
be cleaned after each experiment. After a “normal” experiment use the following procedure to clean the
Tissue Organ Bath, chamber, tubing and tissue holders.
1. Turn pressure OFF
2. Use clean Schott Duran bottles
3. Fill with distilled water
4. Turn Pressure ON
5. Rinse the silicone tubing with double distilled water by activating the Fill & Empty Control several
times.
6. Change the tubing after use of unknown or toxic chemicals, or if any kind of hydrophobic reagent has
been used.
NOTE: Only works when a tissue chamber is in place
IMPORTANT:
It is VERY important that the tubing being used is the original tubing from DMT. The
quality, diameter and length are VERY important in order to insure the correct draining
and filling of the chamber.
Содержание 750 TOBS
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