DMT 720MO, Руководство пользователя

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7 TISSUE BATH MYOGRAPH SYSTEM - 720MO - USER GUIDE
CHAPTER 3 - EXPERIMENTAL SET-UP
This chapter contains experimental set-up for the Tissue Bath. For dissection of a vessel, please see Procedures for investiga-
tions of small vessels using a small vessel Myograph by M.J. Mulvany.
3.1 Mounting protocol
Below is a short description of how to mount a tissue ring preparation on the mounting pins of the 720MO system. DMT deliver
the 720MO system with 200µm pins allowing the user to mount ring preparation with an internal diameter from 450µm and up.
For really large tissue ring preparation with the ability to make high force contractions the 200µm pins may be too thin. DMT can
delivery mounting pins with the following thickness:
L-shaped mounting pins (Diameter): 200µm, 250µm, 300µm and 400µm.
1. Fill the chamber with a given buffer (5-7ml) (see appendix 1 for Buffer recipes). DO NOT FILL THE CHAMBER TO THE EDGE
OF THE CHAMBER
.
2. Make sure that the tissue holding pins are properly aligned.
3. Use the micrometer to get the pins close together as possible without touching each other.
4. Slide your tissue ring preparation onto the two mounting pins.
5. Slowly move the pins apart using the micrometer until the force reading is increasing.
6. The tissue is now mounted and ready for a normalization protocol (see chapter 3.2) finding the optimal pre-load tension for
the mounted tissue
7. Place the micrometer at the position giving the optimal pre-load tension on the mounted tissue.
8. Continue with a standard start (see chapter 3.3).
9. The mounted tissue is now ready for experiments.
Properly aligned pins, seen from side and above