9
3.1.2 Mounting step two
• Using forceps to hold the handle segment, transfer excised vessel from Petri dish to the Auto Dual Wire Myograph chamber.
Hold the vessel as close to the proximal end as possible and try to mount the vessel onto the wire.
• If the lumen is shut, try one of the following possibilities:
1. Use the wire to gently push the lumen open (blood streaming out is a good sign).
2. Hold excised vessel about 3 mm from the cut end with one set of forceps and use the other forceps to squeeze the blood
remaining in lumen out through the cut end.
• Pull the proximal end of the excised vessel segment along the wire such that the vessel segment acts as its own feeder to be
feed into the wire into the vessel (figure 3.2 A-C). Be careful not to stretch the vessel segment if the end of the wire catches
the vessel wall.
3.1.3 Mounting step three
• Once the vessel segment is threaded onto the wire, catch the free end of the wire (nearest you) with the forceps and move
the jaws apart.
• While controlling the movement of the wire with the forceps, use the other forceps to gently pull the vessel segment along
the wire until the area of interest is situated in the gap between the jaws. The near end of the vessel segment shall lie about
0.1 mm inside the jaw gap to insure no point of contact (figure 3.3 A).
• Still controlling the free wire end with the forceps, move the jaws together to clamp the wire and in one movement secure
the wire under the near fixing screw on the left-hand jaw. Again in a clockwise direction so that tightening the screw also
tightens the wire (figure 3.3 B).
Figure 3.3 A and B Mounting step 3
A
B
C
B
Figure 3.2 A, B and C Mounting step 2
