Dionex 043185, Руководство по эксплуатации продукта

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IonPac AS9-SC Column Care Document No. 034656C06 Page 4 of 4
ORGANIC CONTAMINANTS
A. Prepare a two bottles with approximately 500 mL each of the following two eluents. In the first bottle (E1) place 100%
acetonitrile. In the second bottle (E2) make a 1M solution of NaCl using deionized water having a specific resistance of
18.2 megohm-cm and then adjust the pH of the solution to pH 2.0 using HCl. Because acetonitrile slowly breaks down to
form ammonia and acetate in acidic aqueous solutions, the column cleaning solution (80% E1/20% E2) is created by
proportionally mixing these two eluents with the gradient pump which then isocratically pumps the resulting cleaning
solution over the columns to be cleaned.
B. Disconnect the anion suppressor from the IonPac AS9-SC Analytical Column. If your system is configured with both a
guard column and an analytical column, reverse the order of the guard and analytical column in the eluent flow path.
Double check that the eluent flows in the direction designated on each of the column labels. Direct the effluent from the
outlet line of the AS9-SC Guard Column to a separate waste container.
CAUTION
When cleaning an analytical column and a guard column in series, ensure that the guard column is placed after the
analytical column in the eluent flow path. Contaminants that have accumulated on the guard column can be eluted
onto the analytical column and irreversibly damage it. If in doubt, clean each column separately.
C. Set the pump flow rate to 2.0 mL/min.
D. If the application eluent contains a salt that is not compatible with 80% acetonitrile, rinse the column for 10 minutes with
deionized water before pumping the cleaning solution over the column.
E. The cleaning solution is created by proportionally mixing 80% E1 plus 20% E2 with the gradient pump. Isocratically
pump this cleaning solution through the column for at least 60 minutes.
F. If the application contains a salt that is not compatible with 80% acetonitrile, rinse the column for 10 minutes with
deionized water before pumping the application eluent over the column.
G. Reconnect the anion suppressor to the AS9-SC Analytical Column and place the guard column in line between the
injection valve and the analytical column if your system was originally configured with a guard column.
H. Equilibrate the column(s) with the application eluent before resuming normal operation.