Dionex 043185, Руководство по эксплуатации продукта

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IonPac AS9-SC Column Care Document No. 034656C06 Page 2 of 4
BASE-SOLUBLE CONTAMINANTS
WARNING
Do not use the standard NaOH procedure described in other
DIONEX documentation for base cleanup of IonPac AS9-SC/AG9-SC Columns.
This procedure will destroy IonPac AS9-SC/AG9-SC Columns
A. Prepare a 500 mL solution of 200 mM Na
2
CO
3
/75 mM NaHCO
3
(see Section 4.1, Preparation of Eluent Concentrates).
B. Disconnect the anion suppressor from the IonPac AS9-SC Analytical Column. If your system is configured with both a
guard column and an analytical column, reverse the order of the guard and analytical column in the eluent flow path.
Double check that the eluent flows in the direction designated on each of the column labels. Direct the effluent from the
outlet line of the AS9-SC Guard Column to a separate waste container.
CAUTION
When cleaning an analytical column and a guard column in series, ensure that the guard column is placed after the
analytical column in the eluent flow path. Contaminants that have accumulated on the guard column can be eluted
onto the analytical column and irreversibly damage it. If in doubt, clean each column separately.
C. Set the pump flow rate to 1.0 mL/min.
D. If your eluent contains a solvent that is not compatible with the cleanup solution of 200 mM Na
2
CO
3
/75 mM NaHCO
3
,
rinse the column for 15 minutes with deionized water before pumping the cleanup solution over the column.
E. Pump the cleanup solution through the column for 30-60 minutes
F. If the application eluent contains a solvent that is not compatible with the cleanup solution, rinse the column for 15
minutes with deionized water. Then pump the application eluent over the column.
G. Reconnect the AMMS III to the AS9-SC Analytical Column and place the guard column in line between the injection
valve and the analytical column if your system was originally configured with a guard column.
H. Equilibrate the column(s) with the application eluent before resuming normal operation.
NOTE
It may not be necessary to use the above cleanup solution. You may find it more beneficial, depending on your sample
matrix, to use a 10X concentrate of your sodium carbonate/bicarbonate or sodium borate eluent. Note that the
maximum solubility of sodium borate is 300 mM.