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BIORUPTOR

PICO

MANUAL

Critical steps for maintenance
and efficient shearing

General warnings

Sonication bath temperature

• Optimal temperature for sonication is 4°C. Sample temperature should not exceed 8°C.

• The Bioruptor Cooler (Cat. No. B02010010, B02010011, B02010012) has to be used to guarantee the

automatic temperature control of the sonication bath during the entire sonication process.

Validated tubes for the Bioruptor

Pico

• DNA shearing: 0.2 ml (Cat.No. C30010020) and 0.65 ml (New Cat. No. C30010011; Old Cat. No. WA-005-

0500) Bioruptor

Microtubes for DNA shearing.

• Chromatin shearing: 0.2 ml (Cat.No. C30010020) and 1.5 ml (Cat.No. C30010016) Bioruptor

Microtubes

and 15 ml (Cat.No. C01020031) Bioruptor

Tubes & sonication beads.

Fitting tubes in the tube holder

• Place the tubes in the corresponding tube holder. Never leave empty spaces in the tube holder. Fill the

empty spaces with tubes containing the same volume of water. Screw the lid on the tube holder without
over-tightening it.

• Carefully place the tube holder on the holding plate.

• During sonication, samples must remain at the bottom of the tube. If needed, briefly centrifuge samples

during sonication after pausing the run.

DON’T

Turn on the instrument without water

Exceed 30 min of total sonication

Tilt the sonication unit

Change water at least once every month

Use deionized or distilled water

QC your system with our DNA QC Kit

Содержание Bioruptor Pico

Страница 1: ...Bioruptor Pico Sonication System Version 3 01_2020 USER GUIDE ...

Страница 2: ...nty Diagenode will cover the expenses to return the repaired or replacement product This warranty covers only manufacturing defects and does not cover any damage caused by misuse lack of compliance to recommendations stated in the manual neglect accidents abrasion or exposure to extreme temperatures chemical solvents or acids Improper or incorrectly performed maintenance or repairs will void the w...

Страница 3: ... 6 Introduction 7 Technical specifications 8 Getting to know you Bioruptor Pico System 9 Equipment installation 12 Installation 13 System Operation 14 Tube holders tubes 20 BIORUPTOR COOLER Safety 23 Design and functions 26 Operation 36 Maintenance 38 Disposal 43 Technical data 44 ...

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Страница 5: ...BIORUPTOR PICO ...

Страница 6: ...at No C30010020 and 1 5 ml Cat No C30010016 Bioruptor Microtubes and 15 ml Cat No C01020031 Bioruptor Tubes sonication beads Fitting tubes in the tube holder Place the tubes in the corresponding tube holder Never leave empty spaces in the tube holder Fill the empty spaces with tubes containing the same volume of water Screw the lid on the tube holder without over tightening it Carefully place the ...

Страница 7: ... mechanical stress to shear chromatin or DNA remove paraffin from FFPE samples disrupt cells and homogenize tissue or disperse chemical component or reduce the size of liposomes Ultrasound waves pass through the sample expanding and contracting the liquid During expansion negative pressures pull the molecules away from one another to form a cavity or bubble This process is called cavitation The bu...

Страница 8: ...0 15 ml sonication accessories Cat No B01200016 Shearing Consumables 0 2 ml Bioruptor Pico Microtubes Cat No C30010020 0 65 ml Bioruptor Pico Microtubes Cat No C30010011 1 5 ml Bioruptor Pico Microtubes Cat No C30010016 15 ml Bioruptor Pico Tubes Cat No C30010017 15 ml Bioruptor Pico Tubes sonication beads Cat No C01020031 Throughput 0 2 ml microtubes 16 samples 0 65 ml microtubes 12 samples 1 5 m...

Страница 9: ...ing to know your Bioruptor Pico system Bioruptor Pico components overview Bioruptor Cooler detailed instructions page 21 Power cable EU Power cable US Sonication unit Sonication bath Motorized lid Tube holder ...

Страница 10: ...g to specification see table to the fill line Change water at least every month and clean sonication tank with a soft tissue Water temperature The water in the sonication bath must be kept at 4 C Ultrasonic waves produced by the Bioruptor Pico generate heat Drop off in sonication efficiency will occur above 8 C To ensure preservation of the samples and to prevent damage to the instrument it is nec...

Страница 11: ... sample tubes in constant rotation and ensures optimal position in the sonication bath during sonication Avoid the immersion of the motor into the water Tube holders Several sizes of tubes can be used with the Bioruptor Pico The minimum and maximum sample volume to be used with each tube is given in the table below TUBE SIZE MINIMUM MAXIMUM 0 2 ml 20 µl 100 µl 0 65 ml 100 µl 100 µl 1 5 ml 100 µl 3...

Страница 12: ...s are designed to be safe under the following conditions Installation overview Indoor use Altitude up to 2 000 meters Operating external temperature 15 C to 25 C do not install the Bioruptor in a cold room Maximum relative humidity 80 Transient overvoltage typically present on the MAINS supply Degree of protection IP31 Power plug must be grounded POLLUTION DEGREE 2 Normally only non conductive pol...

Страница 13: ...em into the connectors Optional Cut the length you need for the output and input flow Make sure there is enough slack 5 Fill the tank of the Bioruptor Cooler with 2 3 liter and the sonication bath of the Bioruptor with 700 ml 6 Plug the valve connection cable into the outlet on the back side of the Bioruptor Cooler and on the Bioruptor Pico 7 Plug the power cord into the outlet and switch on the p...

Страница 14: ...14 BIORUPTOR PICO MANUAL System operation Touch screen Allows the user to easily program the sonication of samples ...

Страница 15: ...Record your protocol Diagenode dots Go back to the top menu Commercial Support Diagenode contact information for commercial questions Guidelines Read Diagenode recommendations for your applications Go Shear Start a new shearing protocol Good Practices Critical steps for Bioruptor maintenance and efficient shearing Settings Visualize and change the parameters of your Bioruptor Pico Information Gene...

Страница 16: ...e are the parameters controlling the shearing process the time ON is the time during which there is sonication the time OFF is the time during which there is no sonication but water circulation the cycle is made up of a time ON and a time OFF 2 If Easy Mode was selected select the tube type youplantouse TheBioruptorwillautomatically swap to the correct frequency for optimal shearing of your sample...

Страница 17: ...iency Visit our Guidelines folder for more information 2 Vortex and then spin your samples 3 Fill all positions of the tube holder with your samples or with water of same volume and place it on the motorized lid 4 Close the lid to start the run The sonication only starts when the cover is closed The cover also prevents from any noise disturbance ...

Страница 18: ...ator lights up in green You can pause or interrupt the shearing process by pressing the button pause or stop respectively If the cover will be opened during a run the sonication will automatically pause Once the cover is again closed the machine continues with the sonication process 3 You will get a notification on the screen once the shearing process ends successfully Click OK to go back to the m...

Страница 19: ... Tube holder for 1 5 ml tubes Cat No B01201140 15 ml Bioruptor Tubes Cat No C30010017 15 ml Bioruptor Tubes sonication beads Cat No C01020031 Tube holder Dock Tube holders tubes The holder is made up of a tube holder and its specific dock To guarantee homogeneity of chromatin or DNA shearing the tube holders should always be completely filled with tubes Never leave empty spaces in the tube holder ...

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Страница 21: ...BIORUPTOR COOLER ...

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Страница 23: ...vice These devices are essential for the safety of the device Their functionality must be ensured with appropriate maintenance activities If relevant there are warning symbols on the device These symbols must always be observed There are safety instructions in this operating manual These instructions must always be observed There are additional specific requirements for the personnel and for the p...

Страница 24: ...erature thresholds are exceeded or undercut or if the container breaks and there is a reaction with the heat transfer liquid The heat transfer liquid safety data sheet specifies all possible hazards and appropriate safety measures for handling the liquid The safety data sheet must therefore be consulted for the intended use of the device Materials All parts coming into contact with the heat transf...

Страница 25: ...pean Union Protective goggles Protective goggles are required for certain activities These protective goggles must comply with the legal requirements for personal protective equipment of the European Union Structure of the safety instructions Danger A safety instruction of the type Danger indicates an immediately hazardous situation This results in death or severe irreversible injuries if the safe...

Страница 26: ...rt damage If there is unexpected damage to the device or accessories inform the carrier immediately so that a damage report is produced and a check of the transport damage can be made Design and function Design of the circulation chiller Front side 1 Top handle 2 Filler neck with cover 3 Top cover 4 Level indicator 5 Control panel 6 Overflow tube 7 Drain connection 8 Front panel with ventilation o...

Страница 27: ...to the Bioruptor 5 Back handle 6 Ventilation openings Controls Mains power switch The mains power switch can be put in the following positions In position I the device is switched on In position O the device is switched off Control Panel and display buttons 1 Display 2 LEDs 3 UP arrow button 4 ENTER button 5 DOWN arrow button 6 Mains power switch 1 2 4 5 6 3 1 2 6 3 4 5 ...

Страница 28: ...D refrigeration 3 Red LED fault Each device has three LEDs with the following functions The yellow LED lights if the 24 Vdc signal from the Bioruptor is present open solenoid valve The blue LED indicates whether the refrigeration unit is active The red LED lights in the event of device faults 3 1 2 ...

Страница 29: ...the maximum liquid level of the machine The bottom arrow indicates the minimum liquid level of the machine 1 Maximum level approx 3 liters 2 Minimum level approx 2 liters 3 3 5 Refrigeration unit The refrigeration unit includes the following components Compressor A hermetically sealed compressor is used in the refrigeration unit The compressor is equipped with overload protection which trips on th...

Страница 30: ... the compressor of the device GWP value indicated in brackets Fill quantity I Fill quantity of the refrigerant tons of CO2 equivalent in brackets PS high pressure I maximum permitted operating pressure on the refrigerant high pressure side compressor condenser PS low pressure I maximum permitted operating pressure on the refrigerant low pressure side expansion evaporation Current consumption Curre...

Страница 31: ...eration on mains power supplies with connected residential areas In the case of unfavorable mains conditions disruptive voltage fluctuations can occur Instructions for Class A digital device USA This equipment has been tested and found to comply with the limits for Class A digital device pursuant to Part 15 of the FCC Federal Communication Commission Rules These limits are designed to provide reas...

Страница 32: ...ent extraction of the vapors Observe the requirements of the device for electromagnetic compatibility EMC Do not cover the ventilation openings WARNING Rolling away falling over of the device Impact crushing Do not tilt the device Place the device on a level non slip surface with sufficient load bearing capacity Engage the castor brake when setting up the device Do not place any heavy parts on the...

Страница 33: ...medium after addition of 0 1 g soda Na2CO3 sodium carbonate per liter of water Any chlorine content in the water must be strictly avoided Do not add any chlorine to the water Chlorine is contained for example in cleaning agents and disinfectants The water must be free of impurities Water containing iron is unsuitable due to rust formation and untreated river water is unsuitable due to algae format...

Страница 34: ... minimum level has been reached the pumps are automatically started Personnel Operating personnel NOTICE Overheating of the compressor Device damage Never operate water cooled device without cooling water DANGER Use of incorrect heat transfer liquid Fire Select a heat transfer liquid with a temperature range 20 K above the temperature range of the application WARNING Overflow of heat transfer liqu...

Страница 35: ... Low Level the pumps and the refrigerant unit are not started Fill the device with heat transfer liquid 3 Carefully pull up the cover of the filler neck do not turn 4 Fill the heat transfer liquid into the filler neck carefully Monitor the level indicator Fill the device up to the maximum fill level The pumps start when the minimum level is reached If necessary use a funnel for the filling The lev...

Страница 36: ...d if necessary Monitor the level indicator for this Default display and set point temperature 1 Press the ENTER button to reach set point menu from the default display of the actual temperature If no button has been pressed for longer than 4 seconds you exit from the set point menu 2 Select a set point temperature between 2 C and 20 C using the arrow buttons 3 The changed value or setting is appli...

Страница 37: ...ration mode Sleep mode The Display shows SLEP alternating with the bath temperature The device goes into Sleep mode if no 24V signal from the Bioruptor is received for a period of time of more than 16 hours In Sleep mode the compressor and the pumps are turned off If a display button is pushed during Sleep mode the device goes into normal operation mode ...

Страница 38: ...uid are at room temperature before touching them Also note the following Before all maintenance work you should ensure that decontamination of the device has been performed if it came into contact with hazardous materials Maintenance intervals The maintenance intervals described in the following table must be complied with The following maintenance work is mandatory before every longer unsupervise...

Страница 39: ... front cover by holding underneath with both hands and pulling the grating to the front Remove the front cover slowly and carefully to prevent damage 3 Brush off or vacuum the condenser 4 Remount the front cover carefully Checking the heat transfer liquid Soiled or degenerated heat transfer liquid must be replaced Replace fuse 1 Switch off the device 2 Remove power cord from the main power socket ...

Страница 40: ...ng the top handle and unscrewing the two screws at the back of the device 3 Unscrew mesh filter holder counter clockwise 4 Remove the metallic mesh filter and clean it 5 Mount the mesh filter back in the holder and screw it back in the by pass block clockwise 6 Mount top cover and top handle back in the device ...

Страница 41: ... the event of warnings Warnings can be cancelled using the ENTER button after rectification of the cause of the fault Procedure in the event of errors A two tone signal is output if any error occurs The red LED on the device also lights In the case of an error switch off the device at the mains power switch If the error occurs again after restarting the device note the error code and contact the S...

Страница 42: ...e before draining Also note the following Observe the regulations for disposal of the used heat transfer liquid 1 Switch off the device 2 Connect the end of an 8 mm exterior diameter pipe to the self closing drain connector of the device Place the other end of the pipe in a container with appropriate capacity under the level of the device 3 Let the water flow until the device is completely empty ...

Страница 43: ...oning is only permitted by a specialist Global Warming Potential GWP comparisons CO2 1 0 according to IPCC IV time horizon 100 years also basis for EU Fluoride Gases Directive 517 2014 EC Type and fill quantity of the refrigerant can be seen on the rating plate Device disposal The following applies for Europe The device must be disposed of according to EU Directive 2012 19 EC Disposing of packagin...

Страница 44: ... of soiling 2 Clearance from surroundings front and rear sides 20 cm Overvoltage Overvoltage category II and transient overvoltages according to category II Protection class for electrical operating equip ment DIN EN 61 140 VDE 0140 1 1 Classification according to DIN 12 876 1 class designation identification I NFL non flammable liquids only Display 7 segment LED Display resolution 1 C Adjustment ...

Страница 45: ...nt temperature for the measurement is 20 C water was used as heat transfer liquid Hydraulic circuit BC 100 Fill capacity l 2 3 Maximum flow rate l min water 20 C 2 Supply and return connections Quick connector ext hose diameter in mm 8 Drain connector Self closing quick connector ext hose diameter in mm 8 Overflow tube Tube int hose diameter in mm 10 Maximum power consumption BC 100 kW 230 V 50 Hz...

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Страница 48: ...www diagenode com ...

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