DeNovix CellDrop, Руководство пользователя

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8
Chamber Height Options
The CellDrop uses three chamber heights to enable measurements over a wide dynamic concentration
range. The 100 um chamber height is equivalent to the 0.1 mm depth used on standard hemocytometers.
Use a modified protocol with the appropriate chamber height when the cell solution is expected to be
dilute or dense. This ensures that only one aliquot of sample will be needed. If the cell solution is out of
range for the protocol in use, the software will direct the user to reload a fresh aliquot using the required
volume.
Refer to the tables below for guidance regarding which chamber height to use based on the expected
sample cell count or cells/mL.
The equation used to determine the equivalent hemocytometer count is as follows:
Cells/mL
Equivalent cell count=
_______________________________________
(Dilution factor/# of squares counted) *10,000 cells/mL)
Minimum and Maximum Recommended Concentrations
Gap Height Required Volume Minimum Cells/mL
Maximum Expected
Cells/mL
400 um 40 uL 7 x 10 2 5 x 10 4
100 um 10 uL 5 x 10 4 1 x 10 7
50 um 5 uL 1 x 10
7
2.5 x 10
7
Data Storage
CellDrop models include a solid state drive with 60 GB or more of storage capacity, sufficient for several
thousand results. Should the memory approach capacity the user will be alerted. Data may be deleted
from the CellDrop at any time from the Data App. If the limits are exceeded, old data will be automatically
deleted on a first-in, first-out basis.
Screen Saver
The backlight for the screen will automatically turn off after a user-defined period of inactivity. Adjust the
value in the Settings App.
Preview Suspend
The CellDrop live preview on the Count screen is suspended is the arm is left in the down position for
longer than a normal sample is viewed. The user may resume the preview when desired.