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Page 10 

Analysing Plant Seeds  

To count non-touching seeds use 

Count

 in the 

General 

Control 

Window. 
To count seeds, some of which touch, use the 

Seed Count 

Control Window.  
To analyse the shape of non-touching seeds use the 

Object

 

Control Window. 

How to Use Seed Count 

The 

Seed Count

 Control Window provides a method for 

counting seeds which touch, using an average area method. For 
this to work, some seeds should not touch. Also the degree of 
overlap of touching seeds should be small, for the overlap area 
will create a systematic error in the results. 
1. 

Acquire seed image, ideally with minimal seed overlap,  
uniform background, good contrast and minimal shadow.   

2. 

Set the threshold using the standard colour picking tool. 

3.  Click on 

+

 

Pick Seed

 and 

4.  Click on one single seed clearly separated from the rest. 
5.  Repeat steps 3 and 4 on several different isolated single 

seeds. 
Each time you do this the Control Window calculates the 
running average and total seed area, and  from these two it estimates the total 
number.  
From the measured variance in the individual seed areas, and other paramters, it 
also estimates a statistic representing the uncertainty in the total count, shown as 
one standard deviation.  

6.  Continue repeating steps 3 and 4 until you either run out of separate seeds, or the 

statistic ceases to improve significantly. 

7. 

Click on “

Add results to the measurement window. 

This copies the last row of 

data across to the Measurement window. 

If you have another image of more of the same sample of seeds then start again at 
step 1. 
Your measured results will then accumulate in the Measurement Window 

Options 

Minimum area: 

use this to eliminate noise by entering a minimum object size

Include previous images

: Includes results from earlier images when calculating the 

running average area.  

 

Log data

. Saves a record of every line appearing in the Control Panel results window. 

This is saved into the Measurements Results window  
Check this for a complete record of all the data. This might be appropriate for quality 
control. 
 

 

 

The Seed Count Control  

Window

 

Содержание WinDIAS 3

Страница 1: ...WinDIAS 3 Image Analysis System Quick Start Guide version 3 3 Delta T Devices Ltd ...

Страница 2: ...Page 2 Overview USB video Import TWAIN Import TWAIN Scan to file Copy to file Scanner Digital camera Entry Note Not all TWAIN devices will work ...

Страница 3: ...amera Optimise camera settings If using conveyor set shutter speed to about 10 msec to minimise mains flicker Pick colours Long Leaf Live video 1 Optimise and save camera settings 2 4 5 6 7 8 9 10 6 Video Camera Operations 3 Conveyor Live Video NGW 15 4 2011 and resolution Enable Video Pause Video to allow IDS uEye camera settings to be saved within Advanced Camera Settings ...

Страница 4: ...area of interest 8 Define healthy leaf Define diseased leaf Area mode Specify area Measure Z o o m Save results to file D r a g C a l i b r a t e A r e a o f i n t e r e s t I m a g e P r i m a r y T e r t i a r y Aquire image Click here Then click here To Measure Diseased Leaf Area using a Video Camera Results Window Image Window Control Window Undo ...

Страница 5: ...splay the CD Menu If this does not happen then from the Start menu browse to the CD and Run Setup exe If running Windows Vista or later you may be asked to confirm to run Setup 3 Install WinDIAS Select the link to the Installation Guide and read it Then when you are ready click the Install WinDIAS 3 link 4 Install the Video Camera driver Refer to the on screen guidelines and instructions to guide ...

Страница 6: ... Video Advanced Camera Config Image parameters ensure Auto Gain is disabled In Image Processing enable Colour Correction In Color Settings with a live image of a black and white or grey target select One shot After a few seconds the camera finds the best correction and the option changes to Disabled Note The image must not saturate not RGB 255 255 255 all over See also the WinDIAS Manual To change...

Страница 7: ...s its image file into WinDIAS 5 To save an image for batch processing later ensure the WinDIAS colour picking overlays are turned off then select File Export to save in BMP format See also Batch Processing 6 Lighting Cover windows Poorly controlled illumination can dominate the accuracy of your results Variations in lighting may also affect the accuracy of leaf images from flatbed scanners due to ...

Страница 8: ...esults back into the same folder If you previously saved a WinDIAS wd3 format image file to this folder with the name Reference wd3 then WinDIAS will find it and use the settings from that file to process all the other images in that folder If no image file called Reference wd3 is found in the folder WinDIAS will use its current threshold settings and magnification Warnings If no threshold setting...

Страница 9: ... called Results txt along with a second Settings txt file The Results txt file is formatted as tab separated values for ease of importing into spreadsheets such as Excel The Settings txt file contains the reference file name and path date and time and the mid value and range for each of the colour zones picked Tip To extract a record of the colour threshold settings from any wd3 image file save a ...

Страница 10: ...running average and total seed area and from these two it estimates the total number From the measured variance in the individual seed areas and other paramters it also estimates a statistic representing the uncertainty in the total count shown as one standard deviation 6 Continue repeating steps 3 and 4 until you either run out of separate seeds or the statistic ceases to improve significantly 7 ...

Страница 11: ...Page 11 Seed Counting via average area method 1 2 6 Prepare to measure next seed Click on seed to measure it 4 Colour pick to identify all seeds Measurement appears here as a row Repeat steps 3 4 3 5 ...

Страница 12: ... weighing The standard deviation σr of the count is derived1 as follows 𝜎𝑟 2 n𝛾2 1 𝑛 𝑝 Where 𝛾 𝜎 𝑎 σ standard deviation of area of the individually measured seeds a average area of the individually measured seeds n estimated total number of seeds Total Area a p number of individual seeds identified and measured Conveyor Operations WinDIAS can automatically grab and measure the area of leaves fed i...

Страница 13: ... picking thresholds until you are satisfied 11 Clear any unwanted results using the delete button 12 Enable Log data in the Area Control Window and name a results file in the Save As dialog 13 Feed leaves carefully into the conveyor one at a time See also the online Help and WinDIAS User Manual How to Measure Long Leaves Use this for leaves which are too long to be grabbed all at once The Long Lea...

Страница 14: ...ley and lock it in place Re tension the drive band to eliminate slip Do not over tighten Keep all liquids away from the drive band and pulleys Belt Removal De tension the belts Remove the line guides Unlock and release the roller shafts Lift out each belt and its pair of rollers On re assembly locate the bottom roller inside the rubber drive band Belt Renewal and Cleaning Cut the new material to l...

Страница 15: ...ure ring can be used to lock the size of the aperture Sources of information WinDIAS 3 On Line Help Documents installed on your Desktop along with WinDIAS 3 WinDIAS 3 3 User Manual uEye Camera User Manual WinDIAS 3 3 Quick Start Guide this document See the video at https youtu be HbXFOPJ5Jrc Aperture Focus ...

Страница 16: ... likely cost and delay We normally expect to complete repairs within one or two weeks of receiving the equipment However if the equipment must be forwarded to our original supplier for specialist repairs or recalibration additional delays of a few weeks may be expected For contact details see below Technical Support Users in countries that have a Delta T distributor or technical representative sho...

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