Page 10
Analysing Plant Seeds
To count non-touching seeds use
Count
in the
General
Control
Window.
To count seeds, some of which touch, use the
Seed Count
Control Window.
To analyse the shape of non-touching seeds use the
Object
Control Window.
How to Use Seed Count
The
Seed Count
Control Window provides a method for
counting seeds which touch, using an average area method. For
this to work, some seeds should not touch. Also the degree of
overlap of touching seeds should be small, for the overlap area
will create a systematic error in the results.
1.
Acquire seed image, ideally with minimal seed overlap,
uniform background, good contrast and minimal shadow.
2.
Set the threshold using the standard colour picking tool.
3. Click on
+
Pick Seed
and
4. Click on one single seed clearly separated from the rest.
5. Repeat steps 3 and 4 on several different isolated single
seeds.
Each time you do this the Control Window calculates the
running average and total seed area, and from these two it estimates the total
number.
From the measured variance in the individual seed areas, and other paramters, it
also estimates a statistic representing the uncertainty in the total count, shown as
one standard deviation.
6. Continue repeating steps 3 and 4 until you either run out of separate seeds, or the
statistic ceases to improve significantly.
7.
Click on “
Add results to the measurement window.
This copies the last row of
data across to the Measurement window.
If you have another image of more of the same sample of seeds then start again at
step 1.
Your measured results will then accumulate in the Measurement Window
Options
Minimum area:
use this to eliminate noise by entering a minimum object size
.
Include previous images
: Includes results from earlier images when calculating the
running average area.
Log data
. Saves a record of every line appearing in the Control Panel results window.
This is saved into the Measurements Results window
Check this for a complete record of all the data. This might be appropriate for quality
control.
The Seed Count Control
Window
