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DelAgua Water Testing Kit Manual | Version 5.1

Using the sterile tweezers, carefully
remove a sterile membrane filter from

the packet. Hold the membrane only by the
edge and do not let the membrane filter touch
anything while it is being transferred to the
filtration apparatus.

Remove the sterile vacuum cup from the
filtration apparatus. Push the filtration

apparatus firmly onto the vacuum cup (see

Appendix E

for assembling the filtration

manifold). Place the assembly in an upright
position in a convenient place in the kit.

DO NOT place the apparatus on the ground
where it may become soiled.

Unscrew the plastic collar and filtration
funnel in order that these may be easily

removed. DO NOT place these on any surface
other than the filtration base.

6

7

8

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Содержание Portable Water Testing Kit

Страница 1: ...The DelAgua Kit Components 1 DELAGUA PORTABLE WATER TESTING KIT USER MANUAL VERSION 5 1...

Страница 2: ......

Страница 3: ...DELAGUA PORTABLE WATER TESTING KIT USER MANUAL VERSION 5 1...

Страница 4: ...ite www delagua org Facebook www facebook com delaguawater Twitter www twitter com del_agua Copies of this manual are available in several languages Please consult our website for current availability...

Страница 5: ...a procedure which should be followed in numerical order Indicates the section continues on the next page Signifies the end of the section i Note The main sections of this manual describe the use and...

Страница 6: ...Contaminated Material 3 7 Absorbent Pads and Dispenser 3 8 Methanol Dispenser 4 Sampling Methods 4 1 Sampling from a Tap 4 2 Sampling from a Lake Reservoir or other Surface Water Sources 4 3 Sampling...

Страница 7: ...APPENDIX L APPENDIX M Spares List Field Checklist Alternative Types of Media that can be used with the DelAgua Kit for the Isolation of Coliform Bacteria Appropriate Sample Volumes for Coliform Analys...

Страница 8: ...ectronic Timer 6 Kit Manual 7 Empty Media Bottles 10 8 Thermometer 9 Calibration Lid 10 Incubator Lid 11 Filtration Manifold 12 Vacuum Pump 13 Sample Collection Cup 14 10 Disposable Pipettes 15 Chlori...

Страница 9: ...cuum Pump 3 Vacuum Pump Connector 4 Vacuum Pump Connection 5 Black Rubber O Ring 1 2 Filtration Apparatus and Components 1 6 7 8 10 9 5 4 3 2 6 Aluminium Gasket 7 Silicone Rings Pair 8 Bronze Disc 9 F...

Страница 10: ...al Battery Connection Cable 3 Trimmer Tool 4 Tweezers 5 Elastic Strap 6 Steel Sampling Cable 1 6 9 12 5 4 3 2 11 10 7 8 7 Handheld Magnifier 8 Lubrication Grease 9 Fuse 10 Bronze Disc 11 Silicone Ring...

Страница 11: ...cooker portable steriliser or autoclave 2 Electric heating element gas burner stove or similar to heat the portable steriliser or pressure cooker 3 Distilled water for alternatives see Appendix G 4 Me...

Страница 12: ...samples should be taken at random points evenly spread throughout the system Where there are main branches and a remote periphery as shown attention should be devoted to both the main branches and rem...

Страница 13: ...s situation demands Testing needed when environmental conditions change or when an outbreak or increase in waterborne disease occurs Deep tube well with handpump Once initially Thereafter as situation...

Страница 14: ...n a low oxygen atmosphere for example in the closed vacuum cup formaldehyde gas is produced as a by product of combustion Formaldehyde gas is a very effective disinfectant i Note Methanol is expensive...

Страница 15: ...slightly to prevent methanol running onto your hand Methanol is extremely flammable when in contact with a naked flame Caution Filtration apparatus will be hot Be careful when handling Allow the meth...

Страница 16: ...hen add this to the beaker Stir to thoroughly mix the broth 3 2 Preparation of Culture Medium in the Laboratory You will need the following items 1 38 1g of Membrane Lauryl Sulphate Broth MLSB 2 Disti...

Страница 17: ...sed contents and the date and batch of medium Pour approximately 50ml no less than 40ml of culture medium into each of the 10 polypropylene bottles This provides sufficient medium in each bottle to ca...

Страница 18: ...blets in the kit to check that the pH of the water is between 7 0 and 7 8 If it is not it will be necessary to find an alternative source of water Measure out 500ml of clean water in a beaker Add 38 1...

Страница 19: ...m should now be sterile Sterile MLSB will be stable for up to 6 months if stored in a refrigerator between 4 and 6 C Alternatively the medium can be stored for up to 3 months in a cool dark place If t...

Страница 20: ...t Caution To minimise the risk of infection from contaminated materials take care not to touch contaminated membranes directly with your hands DO NOT eat drink or smoke while handling contaminated mat...

Страница 21: ...hot weather To dispense methanol lever the dispensing nozzle into the upright position with the tip of the flame sterilised tweezers Be sure to close the dispensing nozzle after using the kit as the m...

Страница 22: ...sample is representative of the water in the supply pipes Take a water sample with the non sterile sample cup as shown in the image to the right Rinse the cup twice with the sample water before takin...

Страница 23: ...e cup about 30cm below the surface of the water and scoop up the water sample as shown in the top right image This scooping action ensures that no external contamination enters the sample cup In areas...

Страница 24: ...p Pass the other end of the cable through the loop and pull firmly Alternatively the clip on the end of the cable can be fastened to the hole If necessary increase then length of the cable by attachin...

Страница 25: ...ymbol shown above indicate there are video sequences online to accompany the manual instructions Case Studies See examples of the DelAgua Kit in use all over the world To work with us on your own case...

Страница 26: ...out thermotolerant coliform analysis see Section 5 4 The first tests that should be carried out on a drinking water sample are the determination of chlorine residual pH and turbidity The results from...

Страница 27: ...e residual mg litre and pH by holding the comparator up to daylight and matching the colour in the cells with the standard colour scales If the colour falls between two standard colours then it will b...

Страница 28: ...olve the tablet Leave the colour to develop for 10 minutes Read the total chlorine residual mg litre by matching the colour in the cells with the standard colour scale as shown in the image below Subt...

Страница 29: ...d of the tube at the black circle printed on the yellow base of the tube this is the marker Ensure that there is good illumination available If possible hold the turbidity tube over a white surface th...

Страница 30: ...ally and read the turbidity using the graduations on the side of the tube as shown in the image to the left The result is the value of the line nearest the water level 4 5 i Note The graduations follo...

Страница 31: ...re expressed as colony forming units per 100ml of water CFU 100ml 5 4 1 Introduction Thermotolerant coliforms are of sanitary significance when present in drinking water supplies Users should refer to...

Страница 32: ...lt to give a count per 100ml of the original sample The selection of the most appropriate sample volume for a given source treatment plant or distribution system is normally best made in the light of...

Страница 33: ...5 seconds and leave to cool i Note Once the bottle of culture medium has been opened it is recommended that the contents are used within one day It is not advisable to use the medium in one bottle ove...

Страница 34: ...Remove the sterile vacuum cup from the filtration apparatus Push the filtration apparatus firmly onto the vacuum cup see Appendix E for assembling the filtration manifold Place the assembly in an upr...

Страница 35: ...g them to come into contact with any external objects Hold the funnel between the thumb and forefinger to ensure that the collar will not slip off and that the fingers do not come into contact with th...

Страница 36: ...on and continue adding the sample Rinse the sample cup once with the water before taking the sample Take care not to allow external contamination e g dirt and debris to enter the sample cup Insert the...

Страница 37: ...Unscrew the collar and remove the funnel and collar with one hand Using the sterilised tweezers in the other hand lift the membrane carefully from the filtration base Hold the membrane by the edge on...

Страница 38: ...in a petri dish This demonstrates effective sterility of the plates and media which ensures that any positive results are NOT originating from contaminated plates and or media Sample Negative Filter...

Страница 39: ...stressed due to environmental exposure For these types of waters it is beneficial to leave processed membranes for 4 hours after the last sample has been processed before switching on the incubator I...

Страница 40: ...If the stack blank is positive then either the media or the plates could be contaminated A negative 1 2 Squares 3mm 3mm Yellow colonies i Note It is recommended that the mains supply option be used w...

Страница 41: ...hown which have a diameter of between 1mm and 3mm If necessary use the hand lens provided See Appendix L for more information on counting colonies Convert the count into number of thermotolerant colif...

Страница 42: ...place i Note When using the kit in low temperature environments e g less than 10 C the maximum number of 18 hour incubation cycles on one battery charge SHOULD NOT exceed 3 6 Care and Maintenance of...

Страница 43: ...l components of the filtration apparatus including the vacuum and sample cups and to sterilise the apparatus Avoid scratching the comparator and turbidity tubes They rely on an adequate transmission o...

Страница 44: ...ratus 2 Apply a smear of silicone grease to the black rubber O ring 3 Charge the internal battery fully at the end of each week 1 Check the incubator temperature and recalibrate if necessary 2 Clean a...

Страница 45: ...0 45 Filters 38 1g MLSB Tub Pad Dispenser 250pk DPD No1 Tablets non rapid 250pk DPD No3 Tablets non rapid and 250pk Phenol Red Tablets non rapid Consumables Pack with Rapid Tablets 200 Plates Includes...

Страница 46: ...ed A repair kit is available from DelAgua Contact an electronics technician to carry out repairs or contact DelAgua If the lights DO NOT illuminate connect the incubator to the charging unit and conne...

Страница 47: ...ts do illuminate there could be a fault with the charger If the charger is fitted with a 3pin UK style plug the main fuse may have 7 1 3 The Charger Unit is Connected to the Incubator and the Mains El...

Страница 48: ...it is operating from a mains electricity supply or well charged external 12v battery Switch on the incubator Check the temperature of the incubator and observe over a period of 30 minutes to make sure...

Страница 49: ...or has been recalibrated to read between 43 5 and 44 5 C leave it switched on for at least 3 hours Take note of the temperature at 30 minute intervals to ensure that the temperature is stable The foll...

Страница 50: ...ith our page DelAgua Group Keep up to date with all our latest news offers and updates by following the links below Instagram Follow us at delagua_group Pinterest Join us on Pinterest at DelAgua Group...

Страница 51: ...he DelAgua Kit for the Isolation of Coliform Bacteria Appropriate Sample Volumes for Coliform Analysis Assembly of the Filtration Manifold Daily Report Sheet Alternative Sources of Water for Media Pre...

Страница 52: ...analysis Battery replacement kit containing Battery 12v 9 5Ah Silicone sealant Temperature check kit containing Incubator calibration lid with hole Thermometer Adjuster trimmer tool Electrical repair...

Страница 53: ...re medium 38 1g tub for 500ml of growth medium sufficient for 200 plates Culture medium 500g tub for 6 5 litres of growth medium sufficient for 2 600 plates DPD No 1 Tablets DPD No 3 Tablets Phenol Re...

Страница 54: ...ubator lids Thermometer Other Items Culture medium one bottle per day and a spare Membrane filters Absorbent pads and dispenser DPD No 1 tablets DPD No 3 tablets Phenol red tablets Methanol in dispens...

Страница 55: ...Total Coliforms 44 C Thermotolerant Coliforms Incubation Time 24hrs Yellow colonies Colour density may vary Red pink colourless occasionally blue grey Tergitol TTC Available as membrane pad impregnate...

Страница 56: ...E VOLUME Lakes ponds and other surface waters 10ml 1ml Protected groundwater e g wells and springs 100ml 50ml or 10ml Unprotected groundwater e g open dug wells and springs 50ml 10ml or 1ml Note This...

Страница 57: ...sampling programmes It may be useful to analyse different volumes of the same sample in order to decide the best range in which to count the bacteria It is not necessary to sterilise the filtration a...

Страница 58: ...sc to be placed onto The disc should be smooth side up Place the large silicone ring into the circular space in the gasket Then place the smaller silicone ring on top APPENDIX E Assembly of the Filtra...

Страница 59: ...ion apparatus firmly onto the vacuum cup If this is difficult lubricate the black rubber O ring with silicone grease 5 i Note When storing the filtration manifold it is advisable to lubricate the blac...

Страница 60: ...heet Source of sample Time Colour Odour Turbidity NTU Chlorine mg L pH Thermotolerant Coliforms TTC Free DPD No 1 Total DPD No 3 Combined total free Vol filtered ml No of colonies TTC per 100ml Health...

Страница 61: ...he membrane pads Water from a well protected groundwater source Groundwater from a well protected source is usually suitable for preparing the medium However check the pH of the water before use to be...

Страница 62: ...colonies with your fingers or with everyday objects such as pens and pencils that you may use again for other purposes DO NOT carry out microbiological tests in food preparation areas DO NOT dispose...

Страница 63: ...cuum cup the internal surface of the filter funnel the upper part of the filtration base and the surface of the bronze disc Surfaces in contact with the culture medium e g the internal surface of the...

Страница 64: ...ain bacteria other than coliform bacteria that can grow on MLSB at 37 C The colony characteristics of these background organisms will vary only the coliform bacteria will produce yellow colonies Howev...

Страница 65: ...r as shown above and are also labelled The procedure for checking and calibrating the temperature of the two incubators is the same as described in Section 7 2 for the single incubator kit However the...

Страница 66: ...r run the incubator for more than 18 hours during any cycle Always recharge the battery fully at every opportunity using mains electricity External 12v Battery If you are planning to work in the field...

Страница 67: ...colonies the colonies are smaller in diameter When colonies are fewer they tend to be larger This is because the colonies compete for nutrients and will grow larger where there is no competition If th...

Страница 68: ...P3 1 2 3 P1 F1 LED1 RED LED RED LED LED 2 R2 2 2K DIODE D6 IN5401 DC JACK TEMPERATURE SENSOR THERMOSTAT NORMALLY CLOSED LM35 HEATER R10 4 7K R11 560R Q2 BC337 D1 IN4148 U2A LM2904 R7 10K R8 33K R9 18...

Страница 69: ......

Страница 70: ...HEAD OFFICE The Old Dairy Lower Fyfield Marlborough SN8 1PY United Kingdom Tel 44 0 1672 861 198 Fax 44 0 1672 861 724 INFO DELAGUA ORG WWW DELAGUA ORG...

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