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OXFAM-DELAGUA Water Testing Kit — Users Manual
41
5.4.5 Resuscitation of Bacteria
Once the last sample of the day has been taken, wait for a minimum of 60 minutes
before switching on the incubator (resuscitation time). Try to plan the day so that
the time between processing the first and last sample is not more than 3 hours. This
restricts the resuscitation time to a maximum of 4 hours. In cold weather
resuscitation can be achieved by keeping the samples close to the body (eg. In
hands or upright in a pocket but take care to avoid media leaking from the petri-
dishes).
Resuscitation time is particularly important for chlorinated waters or marine water
where the thermotolerant coliform bacteria are ‘stressed’ due to environmental
exposure. For these types of waters it is beneficial to leave processed membranes
for 4 hours after the last sample has been processed before switching on the
incubator.
5.4.6 Sample Incubation
Incubate the samples for 16 to 18 hours. The incubator is designed to maintain a
temperature of 44°C +/- 0.5°C. Always incubate the petri-dishes with the incubator
and case lids closed to reduce heat loss and save battery power (note that it is not
possible to close the kit lid if you are using the double incubator kit, or if you are
powering the kit using the charger or another external power source). Place the kit
on a chair or table to prevent heat loss through the floor and avoid incubating
samples outdoors during cold weather. In order to maximise battery life, do not
leave the incubator on for more than the incubation period, ie. 16 to 18 hours.
There are 3 alternative power sources for the incubator:
1.
Mains electricity supply via the charger unit
2.
Internal battery
3.
External 12v battery (or your vehicle battery)
It is recommended that the mains supply option be used wherever possible.
When used in this way, the charger unit will operate the incubator and at the same
time charge the battery. If the mains electricity fails the internal battery will
operate the incubator.
Using mains electricity or generator via the charger unit
When using mains electricity, the incubator can be operated and the internal
battery charged simultaneously. If the power fails for any reason,
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Страница 52: ...OXFAM DELAGUA Water Testing Kit Users Manual 51 7 2 Fault Finding Chart...
Страница 56: ...OXFAM DELAGUA Water Testing Kit Users Manual 55 APPENDICES...
Страница 57: ...OXFAM DELAGUA Water Testing Kit Users Manual 56 Appendix A Incubator electronic circuit diagram...
Страница 61: ...OXFAM DELAGUA Water Testing Kit Users Manual 60 Appendix D Daily report sheet...