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OXFAM-DELAGUA Water Testing Kit — Users Manual 

 

35

 

Pour the medium onto the pad 

 
2.

 

Allow the medium to warm to ambient 
temperature before use. Pour enough 
culture medium onto the absorbent pad 
in the petri dish to soak the pad and 
leave a slight excess (approximately 
2.5ml). Try not to let the medium run 
down the side of the bottle onto the 
pad, and do not allow the bottle neck 
to come into contact with any external 
objects. Replace the bottle cap 
immediately. If too much medium 
has been added, drain off the excess 
and wipe up any spillage with an absorbent cloth or tissue. Always ensure that 
a slight excess remains in the petri-dish to prevent the pad drying out during 
incubation. 
 
 

Note: Once the bottle of culture medium has been opened, it is recommended that 
the contents are used within one day. It is not advisable to use the medium in one 
bottle over several days since this can lead to contamination. 

 

 
3.

 

Flame the tips of the tweezers 
with the lighter for 
approximately 5 seconds and 
leave to cool. 

 
 

Flame the tips of the tweezers 

Содержание OXFAM-DELAGUA

Страница 1: ...OXFAM DELAGUA PORTABLE WATER TESTING KIT USER MANUAL WITH INSTRUCTIONAL CD ROM VERSION 4 2 REVISED 2009...

Страница 2: ...OXFAM DELAGUA Water Testing Kit Users Manual 1 OXFAM DELAGUA Portable Water Testing Kit VERSION 4 2 REVISED 2009...

Страница 3: ...OXFAM DELAGUA Water Testing Kit Users Manual 2...

Страница 4: ...terial 21 3 7 Absorbent Pads and Dispenser 21 3 8 Methanol Dispenser 22 4 Sampling Methods 23 4 1 Sampling from a Tap 23 4 2 Sampling from a Lake Reservoir or other Surface Water Source 24 4 3 Samplin...

Страница 5: ...OXFAM DELAGUA Water Testing Kit Users Manual 4...

Страница 6: ...ndix D 60 Daily report sheet Appendix E 61 Alternative sources of water for media preparation Appendix F 62 Additional instructions for operating the dual incubator kit Appendix G 64 Additional instru...

Страница 7: ...OXFAM DELAGUA Water Testing Kit Users Manual 6...

Страница 8: ...g Water Quality Second Edition Volume III The equipment should only be used by trained personnel familiar with those guidelines For more information about the kit and for technical help and guidance p...

Страница 9: ...of the kit are entitled to participate in a one day course at Delagua Water Testing Ltd in the use of the kit The course is free of charge Delagua also offers one and 2 week training courses overseas...

Страница 10: ...s inserted into your CD ROM drive This is the first time we have produced a training CD ROM for the kit and so we would welcome your general comments and any suggestions that would help us to improve...

Страница 11: ...OXFAM DELAGUA Water Testing Kit Users Manual 10 1 The OXFAM DELAGUA Kit Components 1 1 General The kit also includes an electronic timer with its own instructions for use...

Страница 12: ...OXFAM DELAGUA Water Testing Kit Users Manual 11 1 2 Filtration Apparatus and Components 1 3 Contents of the Spares Case...

Страница 13: ...steriliser 3 Distilled water for alternatives see Appendix E 4 Means of measuring distilled water eg Measuring cylinder or graduated beaker For using the kit in the field 1 Methanol for alternatives...

Страница 14: ...mples should be taken at random points evenly spread throughout the system Where there are main branches and a remote periphery as shown greater attention should be devoted to the main branches and re...

Страница 15: ...handpump Once initially Thereafter as situation demands Once initially Thereafter as situation demands Testing needed when environmental conditions change or when an outbreak or increase in waterborn...

Страница 16: ...freight and requires special transport conditions We would recommend that you first try to obtain methanol in country from a pharmaceutical supplier a local hospital or university laboratory If necess...

Страница 17: ...aged by heat Caution Keep the mouth of the sample cup away from your face and the hole uppermost to prevent methanol running onto your hand 5 Allow the methanol to burn for several seconds and when al...

Страница 18: ...tablets Section 5 2 3 Ten polypropylene bottles 60ml 4 Measuring cylinder or graduated flask 5 Clean flask or beaker approximately 1 litre capacity 6 Pressure cooker steriliser or autoclave c 7 Heati...

Страница 19: ...that can be performed in one day using the Delagua kit 5 Replace the screw caps on the polypropylene bottles Make sure the caps are secure but do not tighten Leaving the caps slightly loose prevents...

Страница 20: ...the comparator and phenol red tablets in the kit to check that the pH of the water is between 7 0 and 7 8 If it is not it will be necessary to find an alternative source of water 4 Measure out 500ml...

Страница 21: ...w 6 C a deposit may form which dissolves when the medium is warmed and gently shaken The deposit is caused by the lauryl sulphate coming out of solution If signs of deterioration are observed eg cloud...

Страница 22: ...or 30 minutes using an autoclave steriliser or pressure cooker Alternatively plunge the petridishes pads and membranes into boiling water and heat for at least 30 minutes use a dedicated pan for this...

Страница 23: ...e to avoid spillage Do not overfill the methanol dispenser as it may leak in hot weather To dispense methanol lever the dispensing nozzle into the upright position with the tip of the tweezers To seal...

Страница 24: ...efully clean the mouth of the tap with a clean cloth or tissue to remove any dirt or grease Open the tap and leave water running for at least one minute before taking a sample Do not adjust the flow o...

Страница 25: ...om a Lake Reservoir or other Surface Water Source 1 Where there is safe and adequate access to the source it may be possible to take samples by hand 2 Grasp the sample cup firmly keeping your fingers...

Страница 26: ...f the sample cup Note It is important that you obtain a sample which is representative of the main body of water For example when sampling from a river do not sample the quiet or stagnant areas near t...

Страница 27: ...ng a rope or string to the sample cable Take care not to lose the sample cup 3 Lower the sterile sample cup into the well or tank taking care not to allow the cup to touch the walls of the structure w...

Страница 28: ...the vacuum cup several times with the water that is to be analysed before taking a sample for analysis see Section 4 If the results of the analysis are as follows Free chlorine residual greater than 0...

Страница 29: ...Do not shake the comparator as this will introduce air 4 Immediately read the free chlorine residual mg litre and pH by holding the comparator up to daylight and matching the colour in the cells with...

Страница 30: ...nol Red pH DPD No 1 Free chlorine residual DPD No 1 plus DPD No 3 Total chlorine residual Total Free chlorine Combined chlorine 5 3 Turbidity Analysis Note The turbidity tube covers the range 5 to 2 0...

Страница 31: ...3 Hold the tube vertically and read the turbidity using the graduations on the side of the tube The graduations follow a logarithmic scale with the most critical values marked on the side of the tube...

Страница 32: ...nment Always sterilise the items before disposal or cleaning Keep all non essential personnel particularly children away from the work area when handling contaminated materials Always keep your work a...

Страница 33: ...owever this is not convenient when carrying out both thermotolerant coliform and total coliform analysis on a regular basis A dual incubator kit see Appendix F is available from Delagua Water Testing...

Страница 34: ...s with a smaller volume of the sample and then adjust the result to give a count per 100ml of the original sample The selection of the most appropriate sample volume for a given source treatment plant...

Страница 35: ...lease note these volumes are only a guide They do not represent absolute recommendations to be applied to sampling programmes It may be useful to analyse different volumes of the same sample in order...

Страница 36: ...jects Replace the bottle cap immediately If too much medium has been added drain off the excess and wipe up any spillage with an absorbent cloth or tissue Always ensure that a slight excess remains in...

Страница 37: ...irmly onto the vacuum cup if this is difficult lubricate the black rubber O ring with silicone grease see Section 6 for maintenance of the kit Place the assembly in an upright position in a convenient...

Страница 38: ...the membrane filter grid side facing upwards onto the bronze disc filter support Replace the filter funnel and collar immediately without allowing them to come into contact with any external objects H...

Страница 39: ...Rinse the sterile sample cup once with the water before taking the sample Take care not to allow external contamination eg dirt and debris to enter the sample cup 11 Pour the sample into the filtrati...

Страница 40: ...passed through the filter disconnect the pump from the filtration apparatus Do not allow excess air to be drawn down through the filter once all the water has gone through 13 Unscrew the collar and re...

Страница 41: ...de which relates to details on the daily report sheet A wax pencil or marker pen is suitable for this purpose the writing needs to be easily removed after the tests are complete 16 Place the petri dis...

Страница 42: ...a temperature of 44 C 0 5 C Always incubate the petri dishes with the incubator and case lids closed to reduce heat loss and save battery power note that it is not possible to close the kit lid if you...

Страница 43: ...e the incubator for more than 5 cycles without recharging the battery or run the incubator for more than 18 hours during any cycle Always recharge the battery fully at every opportunity using mains el...

Страница 44: ...he internal battery to discharge 5 4 7 Counting Colonies and Recording Results Note It is important that counting is completed as soon as possible after the petri dishes have been removed from the inc...

Страница 45: ...olonies on a membrane If there are more than 100 colonies on the membrane the number can be estimated by dividing the membrane into sections and counting the number of colonies in one section Multiply...

Страница 46: ...e Do not operate outside in cold weather Always Recharge the internal battery at the end of a period of work in the field Always Leave the battery in a charged state when the kit is out of use or in s...

Страница 47: ...ator 6 3 Filtration Apparatus At the end of each day it is good practice to carefully dry all components of the filtration apparatus including the vacuum and sample cups and to sterilise the apparatus...

Страница 48: ...r of silicone grease to the black rubber O ring 3 Charge the internal battery fully at the end of each week Monthly Weekly maintenance plus 1 Check the incubator temperature and recalibrate if necessa...

Страница 49: ...hould be carried out only by a qualified electronics technician A battery replacement kit is available from Delagua Water Testing Ltd Generally total discharge of the battery is a sign of misuse Go to...

Страница 50: ...harger is damaged Replace with a new unit or organise repair through Delagua Water Testing Ltd Then go to Step 1 Note The battery charger has been selected to deliver the correct output for the intern...

Страница 51: ...charged 12v battery using the lead with crocodile clips supplied with the kit Switch on the incubator unit Do the 2 lights on the incubator console light up brightly Yes Go to Step 12 No The incubator...

Страница 52: ...OXFAM DELAGUA Water Testing Kit Users Manual 51 7 2 Fault Finding Chart...

Страница 53: ...bator The equipment supplied for checking and recalibrating the incubator includes the following items 1 Testing incubator lid with centre hole 2 Thermometer 3 Trimmer tool similar to a small screwdri...

Страница 54: ...on is not necessary and the water may be removed with tissue or a cloth If the temperature is not within these limits follow the recalibration procedure below 7 3 2 Procedure for Recalibrating the Inc...

Страница 55: ...ains electricity supply 6 The following day switch on the incubator and allow to reach a stable temperature If the temperature is not within the correct limit repeat the recalibration process detailed...

Страница 56: ...OXFAM DELAGUA Water Testing Kit Users Manual 55 APPENDICES...

Страница 57: ...OXFAM DELAGUA Water Testing Kit Users Manual 56 Appendix A Incubator electronic circuit diagram...

Страница 58: ...cuum pump Turbidity tubes pair Chlorine pH comparator Tweezers Petri dishes Spares box complete Lighter Consumables Culture medium one bottle per day and a spare Membrane filters Absorbent pads and di...

Страница 59: ...ncubator testing lid with hole Thermometer Adjuster trimmer tool Electrical repair kit containing Electrical circuit Temperature chip Sealant Foam compound Adhesives Filtration apparatus complete Spar...

Страница 60: ...200 tests Culture medium 500g tub for 6 5 litres of growth medium sufficient for 2 600 tests DPD No 1 Tablets x250 DPD No 3 Tablets x250 Phenol red Tablets x250 Consumables for 200 tests Optional Ext...

Страница 61: ...OXFAM DELAGUA Water Testing Kit Users Manual 60 Appendix D Daily report sheet...

Страница 62: ...activity Rainwater Collect a sufficient volume of rainwater in a clean container and leave it to stand overnight to allow any suspended matter to settle out Carefully pour off the water into a separat...

Страница 63: ...acteristics of these background organisms will vary only the coliform bacteria will produce yellow colonies However be prepared for high background counts when performing TC analysis There are 2 addit...

Страница 64: ...ting the temperature of the 2 incubators is the same as described in Section 7 3 for the single incubator kit However the corresponding temperature adjuster screws are located underneath the labels sh...

Страница 65: ...instructions for operating the External Battery Pack The external battery pack can be used with both the Double Incubator kit and Single Lightweight Kit To charge the battery connect the battery char...

Страница 66: ...OXFAM DELAGUA Water Testing Kit Users Manual 65 Appendix I Alternative types of media that can be used with the Delagua kit for the isolation of coliform bacteria...

Страница 67: ...Thermotolerant faecal Coliforms on Petri dish University of Surrey Guildford Surrey GU2 7XH UK Tel 44 1483 689209 Fax 44 1483 689971 E mail sales delagua org techsupport delagua org Web site http www...

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