background image

IX2-700 Dual Intracellular Preamplifier 

  5

OFFSET VOLTAGE CONTROL 

 

This control cancels electrochemical potentials developed between reference 

electrodes and the probe input. Offsetting the output level of the amplifier nulls 
these potentials. The range is ±200 mV. Be careful not to touch this control during 
the experiment, as it will change the baseline. 
 

ELECTRODE IMPEDANCE TEST (Z TEST) 

 

This switch provides a rapid check of electrode impedance. When switched 

on, the square wave signal proportional in amplitude to the electrode impedance, 
appears at the 10 VmBNC connector. A signal level of 1 mV is equivalent to 1 
Megohm of electrode resistance when using a X1 headstage. A X.1 (i.e., divide by 
10 headstage) scales this to .1 mV per megohm referred to the input. 
 

DC CURRENT 10-TURN DIAL 

 

The current dial provides a depolarizing (positive) or hyperpolarizing 

(negative) current selected by the corresponding Positive/Off/Negative polarity 
switch. The range of the current is 100 nA full scale times the headstage multiplier. 
Thus, the X .1 headstage would change the full scale dial reading to 9.99 nA. The 
current control is linear and the readout corresponds to the actual current selected. 
This current is algebraically added to the currents produced by the EXT I BNC 
connector and also the Step Current Command Generator, if selected. 
 

BRIDGE BALANCE CONTROL 

 

The bridge Balance Control is used to null out the effects of stimulus current 

passing through the recording electrode. The bridge is balanced by turning the 
knob on the dial housing. When the bridge is balanced the voltage seen at the 
10Vm connector will be nulled to zero and the resistance of the electrode and cell 
membrane is read on the dial. The range of the bridge balance dial is 100 
Megohms divided by the probe current multiplier. Thus, for the X.1 probe, the 
range will be 1000 Megohms. The bridge Balance is disabled when Z Test is used. 
 

STEP CURRENT 10-TURN DIAL 

 

The step current adds to the Holding Current and has ten times the range. It 

can be on continuously, if gated on and off by a greater than 3 volt level supplied 
to the Gate BNC connectors. 

Содержание IX2-700

Страница 1: ...nual Version 1 1 Dagan Corporation 2855 Park Avenue Minneapolis MN 55407 USA 612 827 5959 Fax 612 827 6535 Email info dagan com Web www DAGAN com Warning This instrument is not designed or intended fo...

Страница 2: ...0 Dual Intracellular Preamplifier 1 TABLE OF CONTENTS Page IX2 700 Block Diagram 2 Introduction and Features 3 Control Description 4 Operation 7 Specifications 11 Accessories 12 Warranty Services Repa...

Страница 3: ...IX2 700 Dual Intracellular Preamplifier 2...

Страница 4: ...ltering electrode impedance testing and a back lighted for dark labs LCD voltage and current meter FEATURES Small Box Probe in five current ranges with automatic decimal point selector on current mete...

Страница 5: ...initiating buzzes until you achieve desired cell penetration Buzz magnitude is a trimpot located under the buzz switch CAPACITANCE COMPENSATION CONTROL This control provides input capacitance neutrali...

Страница 6: ...current is 100 nA full scale times the headstage multiplier Thus the X 1 headstage would change the full scale dial reading to 9 99 nA The current control is linear and the readout corresponds to the...

Страница 7: ...able In addition the Buzz Switch is many times useful for clearing a blocked microelectrode and impaling cells MONITOR MON BNC CONNECTOR Actual currents being passed through the microelectrode are mon...

Страница 8: ...hat the voltage is at ground potential ground voltage trace adjust position on oscilloscope to desired position uncouple voltage channel and return trace to the same position using the DC offset contr...

Страница 9: ...when you switch polarity the bridge is balanced The main disadvantage with a bridge circuit arises from changes in the microelectrode resistance The circuit works on the assumption that the bridge is...

Страница 10: ...scillating IMPALING THE CELL With the system calibrated the electrode in good condition and the amplifier compensated you are ready to penetrate the cell As the barrel of the electrode enters the tiss...

Страница 11: ...from channels 1 and 2 are equal Should you need to adjust the trimpot contact the factory for explicit instructions The Cx control is for cross capacitance nulling to adjust for variations in dynamic...

Страница 12: ...pf 1010 M 1 1 A 10M 50 v 10kHz 10M 2 to 50 M 10 pf 35 pf 1011 M 1 Standard 100nA 100M 50 v 1kHz 100M 20 to 500 M 10 pf 35 pf 1012 M 01 10nA 1G 40 v 100Hz 1G 200 to 5000 M 20 pf 1013 M 001 1nA 10G 35 v...

Страница 13: ...1999 my meter displays channel 1 and 2 voltage and current and channel 1 minus 2 voltage decimal point is automatically set Cross Capacitance Control Cx For ion selective electrode balancing Size 3 1...

Страница 14: ...the four screws on the top cover and slide it backwards PRINTED C1RCU1T CARD REMOVAL Unscrew the desired PC board locking screws Unsnap the connectors and pull them upwards FUSE REPLACEMENT The IX2 7...

Отзывы: