Step
Action
4
Place the Tubing holder with the tubing over the Tube adjustment cavity (2) of
the Delivery arm. Push the tubing down against the bottom of the Tube
adjustment cavity, and then fingertighten the Tubing holder nut. This ensures
the correct length of the exposed tubing end (6).
5
Re-install the tubing holder in the Delivery arm.
6
Check that the Tube sensor is in the correct position for the tube size. The
eluent tubing should be above the center of the collection tube. Use the
sensor control to position the Tube holder.
7
Connection to ÄKTA pure
For
Fraction collector F9-R: Connect the tubing from the Fraction collector
to:
•
port
Frac on Outlet valve V9-O, V9H-O, V9-Os or V9H-Os.
For
Fraction collector F9-R, 2nd: Connect the tubing from the second
Fraction collector to:
•
port
Out 10 on Outlet valve V9-O or V9H-O.
•
port
Out 1 on Outlet valve V9-Os.
Connection to ÄKTA avant
For
Fraction collector F9-R, 2nd: Connect the tubing from the second
Fraction collector to:
•
port
Out 10 on Outlet valve V9-O or V9H-O.
8
Set the delay volume according to the length of the tubing used, according to
the instruction above.
14
ÄKTA avant and ÄKTA pure Instructions 29021462 AD